SKOPOLAMİN İLE İNDÜKLENMİŞ AMNEZİK SIÇANLARDA JASMİNUM SAMBAC YAPRAK EKSTRESİNİN ANTİ-AMNEZİK VE NÖROKORUYUCU ETKİLERİ

Öz

Amaç: Bu çalışma, Jasminum sambac’ın etanollü yaprak ekstraktının skopolamin ile indüklenmiş amnezik sıçanlarda anti-amnezik etkilerini, referans standart olarak Donepezil (5 mg/kg) kullanarak araştırmayı amaçlamıştır.

Gereç ve Yöntem: Etanollü ekstrakt maserasyon yöntemi ile hazırlanmış ve kalitatif ile kantitatif fitokimyasal analizlerle karakterize edilmiştir. Biyoaktif bileşenlerin tanımlanması için GC-MS analizi yapılmıştır. Bilişsel fonksiyonlar; yükseltilmiş artı labirent (EPM), delik tahta testi, Morris su labirenti (MWM) ve aktophotometre  kullanılarak  değerlendirilmiştir.  Ayrıca  asetilkolinesteraz (AChE) aktivitesi ve antioksidan durum belirlenmiştir.

Sonuç ve Tartışma: Fitokimyasal analizler flavonoidler, fenoller, antrakinonlar, saponinler ve kumarinlerin varlığını doğrulamıştır. Toplam fenolik madde miktarı 0.29 mg GAE/g yaprak tozu, toplam flavonoid miktarı ise 0.39 mg QE/g yaprak tozu olarak bulunmuştur. DPPH yöntemiyle 200 µg/ml’de %62.55 inhibisyon (IC₅₀: 109.38 µg/ml) saptanmıştır. GC-MS analizinde antioksidan potansiyele sahip altı bileşen dâhil olmak üzere toplam 29 bileşik belirlenmiştir. Davranışsal testler, kaçış latansında azalma (MWM), transfer latansında düşüş (EPM), keşif davranışında ve lokomotor aktivitede artış ile anlamlı hafıza iyileşmesi (p < 0.05) göstermiştir. Bulgular, AChE inhibisyonu ve oksidatif stresin azaltılması yoluyla nöroprotektif etkiyi desteklemekte olup, Alzheimer hastalığı ve bilişsel bozuklukların yönetiminde ileri araştırmalar gerektirmektedir.

Anahtar Kelimeler

• Alzheimer hastalığı
• amnezi
• anti-amnezik aktivite
• bunama
• Jasminum sambac

ANTI-AMNESIC AND NEUROPROTECTIVE EFFECTS OF JASMINUM SAMBAC LEAF EXTRACT IN SCOPOLAMINE-INDUCED AMNESIC RATS

Öz

Objective: This study investigated the anti-amnesic effects of Jasminum sambac ethanolic leaf extract in scopolamine-induced amnesic rats, using Donepezil (5 mg/kg) as the reference standard.

Material and Method: The ethanolic extract was prepared by maceration and characterized through qualitative and quantitative phytochemical analyses. GC–MS analysis was performed to identify bioactive constituents. Cognitive function was evaluated using the elevated plus maze (EPM), hole board test, Morris water maze (MWM), and actophotometer. Biochemical parameters, including acetylcholinesterase (AChE) activity and antioxidant status, were also assessed.

Result and Discussion: Phytochemical screening confirmed the presence of flavonoids, phenols, anthraquinones, saponins, and coumarins, with total phenolic (0.29 mg GAE/g leaf powder) and flavonoid (0.39 mg QE/g leaf powder) contents. The DPPH assay showed 62.55% inhibition at 200 µg/ml (IC₅₀: 109.38 µg/ml). GC–MS identified 29 compounds, including six with antioxidant potential. Behavioral studies demonstrated significant memory enhancement (p < 0.05), evidenced by reduced escape latency (MWM), decreased transfer latency (EPM), increased exploratory behavior, and improved locomotor activity. The findings suggest neuroprotective effects mediated via AChE inhibition and attenuation of oxidative stress, supporting further investigation for cognitive impairment and Alzheimer’s disease management.

Anahtar Kelimeler

• Alzheimer’s disease
• amnesia
• anti-amnesic activity
• dementia
• Jasminum sambac

Kaynakça

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