IN VITRO EVALUATION OF ANTIOXIDANT AND ANTICHOLINESTERASE ACTIVITIES OF ROSA DAMASCENA MILL. ESSENTIAL OIL AND ETHANOL EXTRACTS

Öz

Objective: Reduced acetylcholine levels, which play a critical role in learning and cholinergic neurotransmission, are closely associated with Alzheimer’s disease. This study comparatively evaluated the in vitro antioxidant activity, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory effects, and neuroprotective potential of essential oil obtained from Rosa damascena Mill. grown in the Isparta-Gönen region and the ethanol extract prepared from its dried flowers.

Material and Method: Antioxidant activity was determined using DPPH and ABTS radical scavenging assays, while anticholinesterase activity was assessed by the Ellman method.

Results and Discussion: The essential oil exhibited strong antioxidant activity with IC₅₀ values of 0.9 μg/ml (DPPH) and 1.1 μg/ml (ABTS), whereas the ethanol extract showed IC₅₀ values of 2.6 μg/ml (DPPH) and 2.5 μg/ml (ABTS). The essential oil also demonstrated higher enzyme inhibition (AChE: 85.1%, BChE: 80.6%) compared to the ethanol extract (AChE: 74.5%, BChE: 69.9%). Overall, the essential oil showed superior antioxidant and anticholinesterase activities. These findings support the traditional use of rose oil for memory enhancement and suggest its potential as a natural source for pharmaceutical formulations targeting neurodegenerative disorders.

Anahtar Kelimeler

• Acetylcholinesterase
• Alzheimer's disease
• antioxidant activity
• butyrylcholinesterase
• Rosa damascena Mill.

ROSA DAMASCENA MİLL. UÇUCU YAĞI VE ETANOL EKSTRESİNİN ANTİOKSİDAN VE ANTİKOLİNESTERAZ AKTİVİTELERİNİN İN VİTRO OLARAK DEĞERLENDİRİLMESİ

Öz

Amaç: Öğrenme ve kolinerjik iletimde önemli rol oynayan asetilkolin düzeyindeki azalma, Alzheimer hastalığı ile yakından ilişkilidir. Bu çalışmada, Isparta-Gönen bölgesinde yetişen Rosa damascena Mill.’den elde edilen uçucu yağ ile kurutulmuş çiçeklerinden hazırlanan etanol ekstraktının in vitro antioksidan aktivitesi, asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) inhibisyonu ile nöroprotektif potansiyeli karşılaştırmalı olarak incelenmiştir.

Gereç ve Yöntem: Antioksidan aktivite DPPH ve ABTS radikal giderim yöntemleriyle, antikolinesteraz aktivite ise Ellman yöntemi ile değerlendirilmiştir.

Sonuç ve Tartışma: Uçucu yağın IC₅₀ değerleri DPPH için 0.9 μg/ml ve ABTS için 1.1 μg/ml olarak belirlenirken, etanol ekstraktında bu değerler sırasıyla 2.6 ve 2.5 μg/ml bulunmuştur. Uçucu yağın enzim inhibisyon oranları AChE için %85.1 ve BChE için %80.6 iken, etanol ekstraktında AChE %74.5 ve BChE %69.9 olarak saptanmıştır. Sonuç olarak uçucu yağ, her iki parametrede de daha yüksek aktivite göstermiştir. Bulgular, geleneksel olarak hafıza güçlendirici olarak kullanılan gül yağının kolinesteraz inhibisyonu yoluyla nöroprotektif etki gösterebileceğini ve nörodejeneratif hastalıklara yönelik farmasötik ürünlerde potansiyel taşıdığını ortaya koymaktadır.

Anahtar Kelimeler

• Alzheimer hastalığı
• antioksidan aktivite
• asetilkolinesteraz
• butirilkolinesteraz
• Rosa damascena Mill.

Kaynakça

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