DIANTHUS ANCYRENSIS'İN ANATOMİSİ VE ANTİOKSİDAN KAPASİTESİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Dianthus ancyrensis Hausskn. & Bornm. (Caryophyllaceae), halk arasında "Ankara karanfili" olarak bilinen, ince gövdeli ve linear yapraklı çok yıllık otsu bir bitkidir. Dianthus türlerinin ağrı kesici, mide ağrıları, kalp hastalıkları ve solunum problemleri gibi çeşitli rahatsızlıklar için geleneksel kullanımları uzun bir geçmişe sahiptir. Önceki çalışmalarla desteklenen bilimsel araştırmalar, Dianthus türlerinin antifungal, antioksidan, antikanser ve antibakteriyel özelliklerini ortaya çıkarmıştır. Gereç ve Yöntem: Bu çalışmada, D. ancyrensis bitkisinin anatomisi ışık mikroskobu ile incelenmiş, antioksidan kapasitesi ise DPPH• ve ABTS•+ radikal temizleme testleri ile değerlendirilmiştir. Bitki materyali Ankara’dan toplanmış ve %70 etanolde maserasyon yöntemiyle ekstre edilmiştir. Anatomik kesitler mikroskopta Sartur reaktifi ile hazırlanarak görüntülenmiş, antioksidan analizlerde galik asit ve trolox referans olarak kullanılmıştır. Toplam fenolik içerik ve IC₅₀ değerleri spektrofotometrik olarak hesaplanmıştır. Sonuç ve Tartışma: Anatomik incelemelerde disk şeklinde olan gövde epidermasında 2 veya 3 yardımcı hücrenin eşlik ettiği stomalar ve basit örtü tüyleri gözlendi. Yaprak merkezi olup, yaprakta iki küçük iletim demetinin eşlik ettiği bir ana iletim demeti mevcuttur. Stomalar üst epidermada bulunurken, orta damar ve yaprak kenarlarında basit örtü tüyleri belirgindir. Orta damar, abaksiyal taraftan floemi çevreleyen sklerenkimatik bir doku içerir ve hem orta damar hem de mezofilde büyük druzlar gözlenmiştir. Ekstrenin toplam fenolik içeriği 216.97 mg GAE/g kuru ekstre olarak ölçüldü. Ekstrenin DPPH• ve ABTS•+ radikal temizleme kapasiteleri sırasıyla 2.68 ve 1.68 mg/ml olarak hesaplandı.

Anahtar Kelimeler

• Antioksidan
• bitki anatomisi
• caryophyllaceae
• Dianthus
• Dianthus ancyrensis
• farmasötik botanik

ANATOMY AND ANTIOXIDANT CAPACITY ASSESSMENT OF DIANTHUS ANCYRENSIS

Öz

Objective: Dianthus ancyrensis Hausskn. & Bornm. (Caryophyllaceae), commonly known as "Ankara karanfili", is a perennial herbaceous plant characterized by a slender stem and linear leaves. Dianthus species have a long history of traditional uses for various ailments including pain relief, stomachaches, heart diseases, and respiratory problems. Scientific research supported by previous studies has revealed the antifungal, antioxidant, anticancer and antibacterial properties of Dianthus species. Material and Method: In this study, the anatomy of the D. ancyrensis was examined by light microscopy and its antioxidant capacity was evaluated by DPPH• and ABTS•+ radical scavenging tests. Plant material was collected from Ankara and extracted by maceration in 70% ethanol. Anatomical sections were prepared and visualised under the microscope with Sartur reagent, gallic acid and trolox were used as references in the antioxidant analyses. Total phenolic content and IC₅₀ values were calculated spectrophotometrically. Result and Discussion: Anatomical observations revealed a disk-shaped stem with stomata and simple covering hairs present on the epidermal layer, accompanied by 2 or 3 subsidiary cells. The leaf displayed centric characteristics, with a main vascular bundle accompanied by two smaller veins. Stomata were located on the upper epidermis, while simple covering hairs were prominent on the midrib and leaf margin. The midrib exhibited sclerenchymatous tissue surrounding the phloem from the abaxial side, and large crystal druses were observed in both the midrib and mesophyll. The total phenolic content of the extract was measured at 216.97 mg GAE/g of dry extract. DPPH• and ABTS•+ radical scavenging capacities of the extract were calculated as 2.68 and 1.68 mg/ml, respectively.

Anahtar Kelimeler

• Antioxidant
• caryophyllaceae
• Dianthus
• Dianthus ancyrensis
• pharmaceutical botany
• plant anatomy

Kaynakça

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