EVALUATION OF THE HEPATOPROTECTIVE EFFECTS OF VITAMIN E AND ALPHA-LIPOIC ACID AGAINST DIMETHOATE TOXICITY IN VITRO

Öz

Objective: In this study, the protective effects of vitamin E (Vit E) and alpha-lipoic acid (LA) against the hepatotoxicity of dimethoate, a widely used organophosphate pesticide, were investigated.

Material and Method: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was applied to evaluate cell viability at 24 and 48 hours. The dichlorofluorescein diacetate test was used to evaluate reactive oxygen species (ROS) formation.

Result and Discussion: Half maximum inhibitory concentration (IC₅₀) values were calculated as 412 µM and 181 µM for 24 and 48 h, respectively. Dimethoate significantly decreased cell viability in HepG2 cells at 250 µM for 24 hours of exposure and at 50 µM for 48 hours of exposure. Cell viability decreased in a dose-dependent manner. Vit E was observed to increase cell viability significantly at 250 µM concentration (p<0.05) at 24 h. On the other hand, according to the 48-hour exposure results, Vit E was found to be protective against 100 and 250 µM dimethoate (p<0.01). Furthermore, at 4 h exposure, Vit E and LA significantly decreased the ROS levels induced by 100 µM dimethoate treatment (p<0.05 and p<0.001, respectively). In 24 h exposure, it was observed that only Vit E significantly decreased (p<0.05) the ROS levels formed by 100 µM and 250 µM dimethoate treatment. The findings showed that Vit E could be a hepatoprotective agent against possible dimethoate toxicity.

Anahtar Kelimeler

• Alpha-lipoic acid
• dimethoate
• hepatotoxicity
• oxidative stress
• vitamin E

Etik Beyan

The authors declare that the ethics committee approval is not required for this study.

E VİTAMİNİ VE ALFA-LİPOİK ASİTİN DİMETOAT TOKSİSİTESİNE KARŞI HEPATOPROTEKTİF ETKİLERİNİN İN VİTRO DEĞERLENDİRİLMESİ

Öz

Amaç: Bu çalışmada, yaygın olarak kullanılan bir organofosfatlı pestisit olan dimetoatın hepatoksisitesine karşı Vit E ve alfa-lipoik asidin koruyucu etkileri araştırılmıştır.

Gereç ve Yöntem: Hücre canlılığını 24 ve 48 saatte değerlendirmek için MTT testi uygulanmıştır. ROS oluşumunu değerlendirmek için diklorofloresin diasetat testi kullanılmıştır.

Sonuç ve Tartışma: IC₅₀ değerleri 24 ve 48 saatlik maruziyet için sırasıyla 412 µM ve 181 µM olarak hesaplanmıştır. Dimetoat, 24 saatlik maruziyet için 250 µM ve 48 saatlik maruziyet için 50 µM'de HepG2 hücrelerinde hücre canlılığını önemli ölçüde azaltmıştır. Hücre canlılığı doza bağlı olarak azalmıştır. Vit E'nin 24 saatte 250 µM dimetoat konsantrasyonunda hücre canlılığını önemli ölçüde artırdığı görülmüştür (p<0.05). Öte yandan, 48 saatlik maruziyet sonuçlarına göre, Vit E'nin 100 ve 250 µM dimetoata karşı koruyucu olduğu bulunmuştur (p<0.01). Ayrıca, 4 saatlik maruziyette, Vit E ve LA, 100 µM dimetoat ile oluşturulan ROS düzeylerini önemli ölçüde önlemiştir (sırasıyla p<0.05 ve p<0.001). 24 saatlik maruziyette, yalnızca Vit E'nin 100 µM ve 250 µM dimetoat maruziyetiyle oluşan ROS seviyelerini önemli ölçüde engellediği (p<0.05) gözlemlenmiştir. Bulgular, özellikle Vit E’nin olası dimetoat toksisitesine karşı hepatoprotektif bir ajan olabileceğini göstermiştir.

Anahtar Kelimeler

• Alfa-lipoik asit
• dimetoat
• E vitamini
• hepatotoksisite
• oksidatif stres

Kaynakça

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