AĞIZ ÜLSERİ TEDAVİSİNDE KULLANIM İÇİN MUKOADEZİF BUKKAL NANOLİF FORMÜLASYONLARININ GELİŞTİRİLMESİ VE İN-VITRO DEĞERLENDİRİLMESİ

Öz

Amaç: Oral ülserler, ağrı, inflamasyon ve rahatsızlık nedeniyle hastaların yaşam kalitesini olumsuz etkileyen yaygın lezyonlardır. Bu çalışmanın amacı, oral ülserlerin lokal tedavisi için hidrokortizon (HC) içeren mukoadezif nanolif formülasyonları geliştirmek ve değerlendirmektir. Gereç ve Yöntem: Eudragit’in farklı tipleri (E100, S100, L100-55) ve polivinilpirolidon (PVP) mukoadezif polimerler olarak kullanıldı. Nanolifler elektroçekim sistemi kullanılarak üretildi. Bukkal uygulamaya yönelik olarak nanolifler; diferansiyel taramalı kalorimetri (DSC), mekanik testler, temas açısı ölçümleri ve ex vivo mukoadezyon çalışmaları ile karakterize edildi. Sonuç ve Tartışma: Nanolifler elektroçekim yöntemiyle başarıyla üretildi. Termal analizler, nanolifler içinde HC varlığını doğruladı. Mekanik ve ıslanabilirlik çalışmaları, nanoliflerin bukkal uygulama için uygun fiziksel özelliklere sahip olduğunu gösterdi. Formülasyonların ex vivo mukoadezyon testleri, E2-HC, L2-HC ve S2-HC için sırasıyla 0.20 ± 0.05, 0.12 ± 0.03 ve 0.14 ± 0.01 mJ cm⁻² değerlerine ulaştı. Mukoadezyon testleri mukozal yüzeye güçlü yapışma gösterdi ve bu da uzatılmış kalış süresi ve etkili lokal ilaç salımı potansiyeline işaret etti. Formülasyonların in vitro salım profillerinde, HC, E2-HC, L2-HC ve S2-HC için sırasıyla 1 saatte %40.26, %47.87 ve %47.03 oranında salındı. Formülasyon, sürekli salım ve uygun mukoadeziv güç göstererek potansiyel klinik uygulanabilirliğini gösterdi. Hidrokortizon yüklü bukkal elektro-eğrilmiş nanolifler, oral ülserlerin tedavisinde yeni ve etkili bir ilaç dağıtım platformu olarak umut vaat etmektedir.

Anahtar Kelimeler

• Bukkal nanolif
• elektroçekim
• hidrokortizon
• oral ülser

DEVELOPMENT AND IN-VITRO EVALUATION OF MUCOADHESIVE BUCCAL NANOFIBER FORMULATIONS FOR USE IN ORAL ULCER TREATMENT

Öz

Objective: Oral ulcers are common lesions that negatively affect patients’ quality of life due to pain, inflammation, and discomfort. The aim of this study was to develop and evaluate mucoadhesive nanofiber formulations containing hydrocortisone (HC) for the local treatment of oral ulcers. Material and Method: Different types of Eudragit (E100, S100, L100-55) and polyvinylpyrrolidone (PVP) were used as mucoadhesive polymers. Nanofibers were fabricated using electrospinning system. Nanofibers characterized by differential scanning calorimetry (DSC), mechanical testing, contact angle measurements, and ex vivo mucoadhesion studies for buccal application. Result and Discussion: Nanofibers were successfully produced with electrospinning. Thermal analyses confirmed the HC within the nanofibers. Mechanical and wettability studies showed that the nanofibers had suitable physical properties for buccal application. In ex vivo mucoadhesion tests of the formulations, values were 0.20 ± 0.05, 0.12 ± 0.03, and 0.14 ± 0.01 mJ cm⁻² for E2-HC, L2-HC, and S2-HC, respectively. Mucoadhesion tests demonstrated strong adhesion to the mucosal surface, indicating potential for extended residence time and effective local drug delivery. In vitro release profiles of formulations, HC released 40.26%, 47.87% and 47.03% at 1 h for E2-HC, L2-HC and S2-HC, respectively. The formulations demonstrated sustained release and favorable mucoadhesive strength, indicating potential clinical applicability. Hydrocortisone-loaded buccal electrospun nanofibers showed promise as a novel and effective drug delivery platform in the treatment of oral ulcers.

Anahtar Kelimeler

• Buccal nanofiber
• electrospinning
• hydrocortisone
• oral ulcer

Etik Beyan

Ethics Committee Approval is not required for this study.

Teşekkür

The authors would like to thank Evonik Industries for kindly providing Eudragit® for research purposes.

Kaynakça

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