INVESTIGATION OF THE ANTIMICROBIAL AND ANTICANCER EFFECT OF THE PLANTS CONSOLIDA ORIENTALIS (GAY) SCHROD., AESCULUS HIPPOCASTANUM L., AND SYRINGA VULGARIS L.

Öz

Objective: This study was designed to investigate the anticancer effects of ethanolic extracts of Consolida orientalis, Aesculus hippocastanum, and Syringa vulgaris on the C6 glioma cell line and the antimicrobial effect on pathogen microorganisms.

Material and Method: Strains used in the study were obtained from the Medical Microbiology Laboratory of Sivas Cumhuriyet University Hospital. Plant extracts were evaluated using microdilution broth method for antimicrobial activity. Anticancer activity was assessed via XTT assay for C6-glioma and HT-22 cell lines. Chemical composition of plant extracts characterized using GC-MS analysis.

Result and Discussion: The MIC values of the microbial strains ranged from 2.5 to >20 mg/ml, according to the findings. The extracts showed no activity against Candida albicans and Pseudomonas aeruginosa at the 20 mg/ml. The effective doses of the plant extracts on the C6 glioma were calculated using the IC₅₀ method, values in the range of 186–500 μg/ml. The cytotoxic effect of the extracts on the HT-22 healthy cell line was found to exceed 500 µg/ml in extracts other than C. orientalis. The lowest MIC value was observed in S. vulgaris extract, and the lowest IC₅₀ value in A. hippocastanum extract.

Anahtar Kelimeler

• Aesculus hippocastanum
• anticancer
• antimicrobial
• Consolida orientalis
• Syringa vulgaris

CONSOLIDA ORIENTALIS (GAY) SCHROD., AESCULUS HIPPOCASTANUM L. VE SYRINGA VULGARIS L. BİTKİLERİNİN ANTİMIKROBİYAL VE ANTİKANSER ETKİLERİNİN ARAŞTIRILMASI

Öz

Amaç: Bu çalışma, Consolida orientalis, Aesculus hippocastanum ve Syringa vulgaris'in etanolik ekstrelerinin C6 glioma hücre hattı üzerindeki antikanser etkisini ve patojen mikroorganizmalar üzerindeki antimikrobiyal etkisini araştırmak amacıyla tasarlanmıştır.

Gereç ve Yöntem: Çalışmada kullanılan suşlar Sivas Cumhuriyet Üniversitesi Hastanesi Tıbbi Mikrobiyoloji Laboratuvarı'ndan temin edilmiştir. Bitki ekstrelerinin antimikrobiyal aktiviteleri mikrodilüsyon broth metodu kulanılarak araştırılmıştır. C6-glioma hücre hattı üzerinde antikanser aktivite XTT yöntemi ile değerlendirilmiştir. Bitki ekstrelerinin kimyasal bileşimi GC-MS analizi kullanılarak karakterize edilmiştir.

Sonuç ve Tartışma: Bulgulara göre, mikrobiyal suşların MIC değerleri 2.5 ile >20 mg/ml arasında değişmektedir. Ekstreler 20 mg/ml’de Candida albicans ve Pseudomonas aeruginosa’ya karşı herhangi bir aktivite göstermemiştir. Bitki ekstrelerinin C6 glioma hücre hattı üzerindeki etkili dozları, IC₅₀ yöntemi kullanılarak hesaplanmış, değerler 186–500 μg/ml aralığında tespit edilmiştir. Ekstrelerin HT-22 sağlıklı hücre hattı üzerindeki sitotoksik etkisinin, C. orientalis dışındaki ekstrelerde 500 µg/ml'yi aştığı tespit edilmiştir. En düşük MİK değeri S. vulgaris ekstresinde, en düşük IC₅₀ değeri ise A. hippocastanum ekstresinde gözlemlenmiştir.

Anahtar Kelimeler

• Aesculus hippocastanum
• antikanser
• antimikrobiyal
• Consolida orientalis
• Syringa vulgaris

Kaynakça

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