TABLETLERDEKİ AMLODİPİN BESİLAT'IN TRİNİTROBENZEN SÜLFONİK ASİT İLE SPEKTROFOTOMETRİK TAYİNİ

Yıl 2001, , 1 - 8, 01.06.2001

Cem Yücesoy Ayşegül (yardımcı) Gölcü

https://doi.org/10.1501/Eczfak_0000000356

Öz

Amlodipin besilatın (AB) tayini için hızlı ve basit bir spektrofotometrik metod geliştirildi. Metod
etken madde ile trinitrobenzensülfonik asitin renkli bir ürün oluşturmasına dayanmaktadır. Reaksiyon
oda sıcaklığında alkali ortamda (pH 10) yürümektedir. Oluşan ürün kloroform fazına ekstre edildikten
sonra 337 nm de absorbansı ölçülmektedir. Ekstraksiyonlu işlemde Beer kanunu 6.0-30.0 g.ml-1 AB
aralığında geçerlidir. Metoddaki bütün değişkenler optimize edilmiş ve metodun uygulanabilirliği AB
içeren tabletler analiz edilerek sınanmıştır. Sonuçların tekraredilebilirliği bağıl standart sapma olarak %
1.34 tür ve analiz edilen tabletlere ilave edilen saf AB için geri kazanım % 99.7 dir. Sonuçlar daha önce
geliştirilen kloranil metoduyla istatistiksel olarak uyumludur.. Elde edilen veriler geliştirilen metodun
tabletlerdeki AB nin analizinde kullanılabileceğini göstermektedir.

Anahtar Kelimeler

Amlodipin besilat tayini, trinitrobenzensülfonik asit, renk reaksiyonu, UV-Görünür bölge spektrofotometrisi, ilaç analizi.

SPECTROPHOTOMETRIC DETERMINATION OF AMLODIPINE BESYLATE IN TABLETS WITH TRINITROBENZENE SULPHONIC ACID

Öz

A rapid and simple spectrophotometric method for the determination of amlodipine besylate was
developed. The method was based on the formation of a colored derivative between the drug and
trinitrobenzenesulphonic acid. The reaction proceeds at room temperature in alkaline media (pH 10).
The product was extracted into chloroform and the absorbance was measured at 337 nm. The Beer law
limits for the procedure with extraction step were between 6.0-30.0 g-ml-1AB. All variables in the method
were optimized and the applicability of the method was examined by analyzing tablets containing AB.
Reproducibility of the results was 1.34 % as relative standard deviation and the recovery for pure AB
added to preanalyzed tablets was 99.7 %. The results were statistically in concordance with that of
chloranil method, which was formerly developed. Data obtained shows that the developed method can be
used for determination of AB in tablets.Reproducibility of the results was 1.34 % as relative standard deviation and the recovery for pure AB added to preanalyzed tablets was 99.7 %. The results were statistically in concordance with that of
chloranil method, which was formerly developed. Data obtained shows that the developed method can be
used for determination of AB in tablets.

Anahtar Kelimeler

Amlodipine besylate determination, trinitrobenzenesulphonic acid, color reaction, UV-vis spectrophotometry, drug analysis.