Karbonmonoksit (CO) Zehirlenmesi ile Ölenlerde ve Sigara İçenlerde Karboksihemoglobin (COHb) ve Methemoglobin (MetHb) Düzeyleri :

Öz

CO zehirlenmesi sonucu ölen kişilerin (n:18) kanında postmor­tem karboksihemoglobin (COHb) tayini Dubovvski'nin spektrofotometrik yöntemi ile yapılmıştır. Ayrıca COHb yanında methemoglobin (MetHb) miktarı da spektrofotometrik yöntem ile araştırılmıştır. Postmortem kan Örneklerinde COHb düzeyi % 63-88 arasında (orta­lama % 73.11 ± 6.90) ve MetHb düzeyi ise % 0.86-4.20 arasında (ortalama % 3.00 ± 0.87) bulunmuştur. Kan örneklerindeki COHb ve MetHb konsantrasyo alarmda, buzdolabı sıcaklığında 2 ay süreli bekletmenin ve heparin ilavesinin anlamlı bir kayba yol açmadığı gözlenmiştir (P > 0.05). Ayrıca CO'e kronik maruz kalan kişilerde (sigara içenlerde n: 100) COHb ve M e t H b düzeyleri aynı yöntemle yapılmıştır. COHb ve MetHb düzeyleri, günde 1-9 tane içenlerde (n: 44) % 1.94 ± 0.11 COHb, % 1.19 ± 0.05 MetHb, 10-20 tane içenlerde (n: 23) % 3.25 ± 0 . 1 9 COHb, % 1.47 ± 0.06 M e t H b ve > 20 tane içenlerde ise (n: 31) % 4.36 ± 0.14 COHb, % 1.55 ± 0 . 1 4 MetHb belirlenmiştir. COHb düzeyi sigara içen tüm gruplarda içmeyenlere göre ( % 0.86 ± 0.05) anlamlı yüksek bulunmuştur (P < 0.01, P < 0.05). bowski's spectrophotometric method. Occurence of methemoglobin (MetHb) in postmortem blood was also investigated by spectrophotometric method. COHb and MetHb levels of postmortem blood samples were found between 63.00-88.00 % (mean 73.11 ± 6.90 %) and 0.864.20 % (mean 3.00 ± 0.87%) respectively. COHb and MetHb levels of the subjects (cigarette smokers n: 100) who exposed to low CO were also determined by the spectrophotometric method mentioned above. COHb and MetHb levels of the smokers were found 1.94 ± 0.11 % COHb and 1.19 ± 0.05 % MetHb who smoked 1-9 cigarettes daily (n: 44), 3.25 ± 0.19 % COHb and 1.47 ± 0.06 % MetHb who smoked 10-20 cigarettes daily (n: 23), 4.36 ± 0.14 % COHb and 1.55 ± 0 . 1 4 % MetHb who smokers in all groups were found significantly higher than the nonsmokers (P < 0.01, P < 0.05)

Anahtar Kelimeler

• Karbonmonoksit, karboksihemoglobin, methemoglobin, zehirlenme, spektrofotometre

COHb and MetHb Levels in Victims of CO Poisoning and Cigarette Smokers

Öz

Postmortem carboxyhemglobin (COHb) levels in the blood of victims (n: 18) suspected of CO poisoning were determined by Dubowski's spectrophotometric method. Occurence of methemoglobin (MetHb) in postmortem blood was also investigated by spectrophotometric method. COHb and MetHb levels of postmortem blood samples were found between 63.00-88.00 % (mean 73.11 ± 6.90 %) and 0.864.20 % (mean 3.00 ± 0.87%) respectively. COHb and MetHb levels of the subjects (cigarette smokers n: 100) who exposed to low CO were also determined by the spectrophotometric method mentioned above. COHb and MetHb levels of the smokers were found 1.94 ± 0.11 % COHb and 1.19 ± 0.05 % MetHb who smoked 1-9 cigarettes daily (n: 44), 3.25 ± 0.19 % COHb and 1.47 ± 0.06 % MetHb who smoked 10-20 cigarettes daily (n: 23), 4.36 ± 0.14 % COHb and 1.55 ± 0 . 1 4 % MetHb who smokers in all groups were found significantly higher than the nonsmokers (P < 0.01, P < 0.05)

Anahtar Kelimeler

• Carbon monoxide, carboxyhemoglobin, methemoglobin, poisoning, spectrophotometry