SCREENING OF ANTI-PEPTIC ULCER ACTIVITY OF JASMINUM SAMBAC

Öz

Objective: The aim of this study was to investigate the antiulcer activity of callus derived metabolites.

Material and Method: The effect of different (2,4-Dicloro-Phenoxyacetic Acid(2,4-D), Indole- 3-Acetic acid, (IAA), Indole-3-Butyric Acid(IBA), Naphthalene Acetic Acid(NAA)) growth hormones and their combination on callus formation, callus texture and weight (fresh and dry) of Jasminum sambac were investigated. In this investigation revealed that the best callus performance seen in individual MS medium containing 1.5 mg/L 2,4-D and combination 1.5 mg/L 2,4-D along with 1.5 mg/L IAA. Antiulcer activity was studied using the pylorus ligation method. A dosage of 200mg/kg b.w used for albino wistar rats was selected from the LD50 study.Ulcer inhibition and ulcer score, gastric volume, pH, free acidity, total acidity and pepsin, carbohydrate and protein ratio were analyzed.

Result and Discussion: Plant has show higher activity in percentage of callus formation in MS media supplemented with single hormone of 1.5 mg/L 2,4-D. The friable callus was observed in single and combination of the hormone concentration. The callus extract also comprehensively decreased the gastric volume, free and total acidity, and improved the pH of the gastric fluid, proving its antisecretory activity. The results of this study showed that the surveyed extracts of the investigated callus exhibited potent antiulcerogenic activity and dose related activity.

Anahtar Kelimeler

• 2,4-D
• callus
• indole-3-acetic acid
• Jasminum sambac
• ulcer score

Destekleyen Kurum

Vels Institute of Science, Technology & Advanced Studies (VISTAS)

Proje Numarası

Nil

Teşekkür

The authors would like to thank Karpagam University, Coimbatore providing with Lab facility to do the research work.

JASMINUM SAMBAC'IN ANTİ-PEPTİK ÜLSER AKTİVİTE TARAMASI

Öz

Amaç: Bu çalışmanın amacı, kallus kaynaklı metabolitlerin ülser önleyici aktivitesini araştırmaktı.

Gereç ve Yöntem: Farklı (2,4-D, IAA, IBA, NAA) büyüme hormonlarının ve bunların kombinasyonlarının kallus oluşumu, kallus dokusu ve Jasminum sambac'ın ağırlığı (taze ve kuru) üzerindeki etkisi araştırılmıştır. Bu araştırmada, 1.5 mg/L 2,4-D ve 1.5 mg /L 2,4-D kombinasyonu ve 1.5 mg / L IAA içeren bireysel MS ortamında en iyi kallus performansının ortaya çıktığı görülmüştür. Antiülser aktivite pilorligasyonu yöntemi kullanılarak çalışılmıştır. Wistar albino sıçanlara uygulanan 200 mg/kg vücut ağırlığı dozu LD50 çalışmasından seçilmiştir. Ülser inhibisyonu, ülser skoru, gastrik hacim, pH, serbest asitlik, toplam asitlik ve pepsin, karbonhidrat ve protein oranı analiz edilmiştir.

Sonuç ve Tartışma: Bitki, 1.5 mg / L 2,4-D tek hormon ile takviye edilmiş MS ortamında kallus oluşumu yüzdesinde daha yüksek aktivite göstermiştir. Gevrek kallus, hormon konsantrasyonunun tekli ve kombinasyonunda gözlendi. Kallus ekstresi ayrıca mide hacmini, serbest ve toplam asiditeyi kapsamlı bir şekilde düşürdü ve mide sıvısının pH'ını iyileştirerek salgı önleyici aktivitesini kanıtladı. Bu çalışmanın sonuçları, araştırılan kallusun incelenen ekstrelerinin güçlü anti-ülserojenik aktivite ve doza bağlı aktivite sergilediğini gösterdi.

Anahtar Kelimeler

• 2,4-D
• indol-3-asetik asit
• Jasminum sambac
• kallus
• ülser skoru

Proje Numarası

Nil

Kaynakça

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