IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC

Öz

Objective: The distinguishing proof and measurement of biomarker β-sitosterol present in a leaf of Calotropis procera and Calotropis gigantea belonging to family Asclepidiaceae was carried by utilizing high performance thin-layer chromatographic (HPTLC) characterization method. In the present research work the Exterinsic Standard Method is used for quantitative assessment.

Material and Method: It assures the accuracy of quantitative investigation and particularly benifit for HPTLC analysis. Aluminum-maintained silica gel 60F254 HPTLC plates of 250μm thickness was used for chromatographic analysis. Toluene: Ethyl acetate: Glacial acetic acid acidic 8:2:0.20 (v/v/v) solvent system was used as mobile phase. Anisaldehyde sulphuric acid is used for derivatisation and was evaluated at 510 nm.

Result and Discussion: β-sitosterol quantification in foundations of plant was observed as 11.63 µg and 10.23 µg for Calotropi sprocera and Calotropis gigantea respectively. The appropriateness and particularity of the established techniques was demonstrated by qualitative and quantative analysis. This established method for estimation of β-sitosterol in the leaf ofCalotropis procera and Calotropis gigantea was proved to be responsive, particular and replicable.

Anahtar Kelimeler

• Calotropis procera
• Calotropis gigantea
• HPTLC
• identification
• quantification

HPTLC İLE CALOTROPIS GIGANTEA VE CALOTROPIS PROCERA YAPRAĞINDAKİ β-SİTOSTEROLÜN TANIMLANMASI VE MİKTAR TAYİNİ

Öz

Amaç: Asclepidiaceae familyasına ait Calotropis procerave Calotropis gigantean yaprağında bulunan biyomarker β-sitosterol'ün ayırt edici kanıtı ve ölçümü, yüksek performanslı ince tabaka kromatografik (HPTLC) karakterizasyon yöntemi kullanılarak gerçekleştirildi. Bu çalışmada, kantitatif değerlendirme için dış standart yöntemi kullanılmıştır.

Gereç ve Yöntem: Yöntem, kantitatif araştırmanın doğruluğunu sağlamaktadır ve özellikle HPTLC analizi için uygundur. Kromatografik analiz için, 250 μm kalınlığında silika jel 60F254 kaplı alüminyum HPTLC plakları kullanıldı. Mobil faz olarak; toluen: etil asetat: glasiyal asetik asit 8: 2: 0.20 (v / v / v) çözücü sistemi kullanıldı. Türevlendirme için anisaldehit sülfürik asit kullanıldı ve 510 nmdalga boyunda değerlendirildi.

Sonuç ve Tartışma: Bitki yapısındaki β-sitosterol miktarının Calotropis procera ve Calotropis gigantea için sırasıyla 11,63 µg ve 10,23 µg olduğu görülmüştür. Kullanılan yöntemlerin uygunluğu ve seçiciliği, nitel ve nicel analizlerle gösterilmiştir. Belirlenen bu yöntemin;Calotropis procera ve Calotropis gigantea yaprağındaki β-sitosterol miktarı tayini için, hassas, seçici ve tekrar edilebilir olduğu kanıtlanmıştır.

Anahtar Kelimeler

• Calotropis procera
• Calotropis gigantea
• HPTLC
• miktar tayini
• teşhis

Kaynakça

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