YENİ İZOLE EDILEN VB_K1 BAKTERİYOFAJININ İZOLASYONU VE ESBL POZİTİF KLEBSİELLA TÜRLERİ ÜZERİNE DUYARLILIĞININ ARAŞTIRILMASI

Öz

Amaç: Klebsiella ile ilişkili enfeksiyonların tedavisinde kullanılan antimikrobiyallerin etkinliğindeki azalma, alternatif tedavi strateji arayışlarını gerektirmiştir. Bu çalışma Klebsiella türlerine özgü litik karakterde bakteriyofaj izolasyonunun sağlanmasını ve alternatif antimikrobiyal ajan olarak kullanım potansiyelinin araştırılmasını amaçlamaktadır.

Gereç ve Yöntem: Ankara deresinden alınan su örneklerinden faj izolasyonu için, Genişlemiş spektrumlu beta laktamaz (GSBL) üreticisi bir adet Klebsiella spp. suşu konak bakteri olarak kullanıldı. Faj zenginleştirme sonrası, olası bakteriyofaj varlığını belirlemek için spot test yöntemi uygulandı. Litik bakteriyofaj varlığı, spot testi pozitif numunelere çift tabaka agar yöntemi uygulanarak doğrulandı. Bakteriyofajın duyarlılığı, 38 klinik GSBL pozitif Klebsiella spp. suşu kullanılarak in vitro spot test ile belirlendi.

Sonuç ve Tartışma: İlk taramada petride 1.00 mm çapında görünür plaklar üreten vB_K1 bakteriyofajı izole edildi. GSBL pozitif Klebsiella spp. suşlarının bakteriyofaj duyarlılığı %73.7 olarak belirlendi. vB_K1 bakteriyofajının Klebsiella suşları üzerinde çok etkili olduğu kanıtlanmıştır. Bununla birlikte, karakterize edilen bakteriyofajın in vitro bakteriyofaj duyarlılığı cesaret verici bir gelişmedir.

Anahtar Kelimeler

• Bakteriyofaj
• direnç
• ESBL
• Klebsiella spp.

ISOLATION OF NEWLY ISOLATED VB_K1 BACTERIOPHAGE AND INVESTIGATION OF SUSCEPTIBILITY ON ESBL POSITIVE KLEBSIELLA SPP. STRAINS

Öz

Objective: The decrease in the efficacy of antimicrobials in the treatment of Klebsiella-related infections necessitated the search for alternative treatment strategies. This study aims to provide isolation of lytic bacteriophage specific to Klebsiella species and to investigate its potential for use as alternative antimicrobial agent.

Material and Method: One Extended spectrum beta lactamase (ESBL) producer Klebsiella strain was used as host bacteria and water samples were collected from river in Ankara for bacteriophage isolation. Spot test method was applied to determine the possible presence of bacteriophage after phage enrichment. To confirm the presence of the lytic bacteriophage, double layer agar method was applied to spot test positive samples. The susceptibility of the bacteriophage was determined using in vitro spot test. 38 clinical ESBL positive Klebsiella spp. strains were used for this analysis.

Result and Discussion: In the initial screening, the vB_K1 bacteriophage producing visible plaques with a diameter of 1.00 mm was isolated in the petri dish. The susceptibility of ESBL positive Klebsiella spp. strains to this bacteriophage was determined as 73.7%. It was proved that vB_K1 bacteriophage is very effective to Klebsiella spp. strains. However, in vitro bacteriophage susceptibility of characterized bacteriophage is encouraging development.

Anahtar Kelimeler

• Bacteriophage
• ESBL
• Klebsiella spp.
• resistance

Kaynakça

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