SİYAH VE BEYAZ CHIA TOHUMLARININ FARKLI YÖNTEMLERLE ELDE EDİLEN EKSTRELERİNİN BİYOLOJİK AKTİVİTE TAYİNİ

Öz

Amaç: Siyah ve beyaz chia tohumlarının biyolojik aktivitelerinin farklı esktraksiyon teknikleriyle elde edilen ekstrelerde tayinlerinin yapılması ve karşılaştırılması.
Gereç ve Yöntem: Farklı tekniklerle elde edilen ekstrelerin biyolojik aktivite çalışmaları, DPPH radikali süpürme potansiyeli testi, TEAC testi ve ORAC testi uygulanarak tayin edilmiştir. Ayrıca metanollü ekstre numunelerinde toplam fenol ve flavonoit miktar tayinleri yapılmıştır.
Sonuç ve Tartışma: Bu çalışmada fenol miktarı siyah chia tohumu için 9,86 mg GAE/g, beyaz chia tohumu için ise 12,69 mg GAE/g olarak belirlenmiş olup, toplam flavonoid miktarı ise siyah chia tohumu için 0,098 mg RE/mL ve beyaz chia tohumu için 0,099 mg RE/mL olarak belirlenmiştir. Ayrıca yapılan antioksidan aktivite testleri göz önüne alındığında, TEAC testi için en yüksek antioksidan aktivite, soğuk sıkım yöntemiyle elde edilen hem siyah hem de beyaz tohum ekstresinde, DPPH için en yüksek aktivite, soğuk sıkım tekniğiyle elde edilen beyaz tohum ekstresinde, ORAC testi için en yüksek aktivite ise Folch tekniğiyle elde edilen beyaz tohum ekstresinde görülmüştür.

Anahtar Kelimeler

• Biyolojik aktivite
• chia
• DPPH
• ORAC
• TEAC

BIOLOGICAL ACTIVITY DETERMINATION OF BLACK AND WHITE CHIA SEED EXTRACTS OBTAINED BY DIFFERENT EXTRACTION METHODS

Öz

Objective: Determination and comparison of the biological activities of black and white chia seeds in extracts obtained by different extraction techniques.

Material and Method: Biological activity studies of extracts obtained by different techniques were determined by applying DPPH radical scavenging potential test, TEAC test and ORAC test. In addition, total phenol and flavonoid amounts were determined in methanolic extract samples.

Result and Discussion: In this study, phenol amount was determined as 9.86 mg GAE/g for black seed and 12.69 mg GAE/g for white seed and the total amount of flavonoids was determined as 0.098 mg RE/mL for black chia seed and 0.099 RE/mL for white seed. In addition, considering the antioxidant activity tests, the highest antioxidant activity for TEAC test was found in both black and white seed extracts obtained by the Cold press method, the highest activity for DPPH was in the white seed extracted obtained by the Cold press method, and the highest activity for ORAC test was observed in the white seed extract obtained by the Folch technique.

Keywords: Biological activity, chia, DPPH, ORAC, TEAC

Anahtar Kelimeler

• Biological activity
• chia
• DPPH
• ORAC
• TEAC

Kaynakça

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