A PRELIMINARY STUDY ON THE ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF OPOPANAX HISPIDUS

Öz

Objective: Opopanax W. Koch genus is included in Apiaceae family. The genus can be distinguishable with its compound basal leaves, glabrous fruits, yellow flowers, tall stem with glochidate and stellate hairs. It is known with the name “Hercules’ all-heal” throughout the world. It was used for the treatment of epilepsy, infertility in women, hemorrhoids and paralysis traditionally. This present research was designed to assess in vitro antioxidant and anti-inflammatory activity of Opopanax hispidus (Friv.) Griseb.

Material and Method: Methanol extracts were prepared from the aerial parts and flowers of the plants. Their antioxidant activities were analyzed through a number of chemical assays: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-l-picrylhydrazyl (DPPH) free radical scavenging assays. Anti-inflammatory activities of the extracts were compared and evaluated using human red blood cell membrane stability testing.

Result and Discussion: Aerial parts exhibited stronger ABTS and DPPH free radical scavenging activities than flowers. Howver, flowers were found to be more active than aerial parts in terms of anti-inflammatory effects (IC50=3,32 mg/ml and 4,75 mg/ml; respectively). These data suggest that flowers and aerial parts of Opopanax hispidus exhibited antioxidant and anti-inflammatory effects. More research studies are necessary to determine the active ingredients in charge of this activity.

Anahtar Kelimeler

• Anti-inflammatory
• Anti-oxidant
• ABTS
• DPPH
• Opopanax hispidus

OPOPANAX HISPIDUS’UN ANTİOKSİDAN VE ANTİ-İNFLAMATUAR AKTİVİTELERİ ÜZERİNE BİR ÖN ÇALIŞMA

Öz

Amaç: Opopanax W. Koch cinsi, Apiaceae familyasında yer alır. Cins, bileşik bazal yaprakları, tüysüz meyveleri, sarı çiçekleri, gloşidat ve yıldızsı tüyleri olan uzun gövdesi ile ayırt edilebilir. Dünya çapında “Herkül'ün her şeyi iyileştireni” adıyla bilinir. Halk arasında epilepsi, kadınlarda kısırlık, hemoroid ve felç tedavisi için kullanılır. Bu araştırma, Opopanax hispidus (Friv.) Griseb'in in vitro antioksidan ve anti-inflamatuar aktivitesini değerlendirmek için tasarlanmıştır.

Gereç ve Yöntem: Bitkinin toprak üstü kısımlarından ve çiçeklerinden metanol ekstreleri hazırlandı. Antioksidan aktiviteleri bir dizi kimyasal deneyle analiz edildi: 2,2'-azino-bis-3-etilbenzotiazolin- 6-sülfonik asit (ABTS) ve 2,2-difenil-l-pikrilhidrazil (DPPH) serbest radikal süpürme deneyleri. Ekstrelerin anti-inflamatuar aktiviteleri insan kırmızı kan hücresi membran stabilite testi kullanılarak karşılaştırıldı ve değerlendirildi.

Sonuç ve Tartışma: Toprak üstü kısımları, çiçeklerden daha güçlü ABTS ve DPPH serbest radikal temizleme aktiviteleri sergiledi. Ancak anti-inflamatuvar etki açısından çiçeklerin toprak üstü kısımlardan daha aktif olduğu bulundu (IC50=3,32 mg/ml ve 4,75 mg/ml, sırasıyla). Bu veriler, Opopanax hispidus'un çiçek ve toprak üstü kısımlarının antioksidan ve anti-inflamatuar etkiler sergilediğini göstermektedir. Bu aktiviteden sorumlu aktif içerikleri belirlemek için daha fazla araştırma çalışması gereklidir.

Anahtar Kelimeler

• ABTS
• anti-inflamatuvar
• anti-oksidan
• DPPH
• Opopanax hispidus

Kaynakça

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