CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF ALCHEMILLA MOLLIS (BUSER) ROTHM. AND ITS CONTITUENTS; HYPEROSIDE AND ISOQUERCETIN

Öz

Objective: Alchemilla mollis (Buser) Rothm. (Rosaceae) is widely distributed in Europe, North Anatolia, Caucasus, North Iran. A commercial drug, "Herba Alchemillae" is obtained from aerial parts of A. mollis, is used for its astringent, diuretic, antispazmodic properties as well as for treatment of excessive menstruation and wounds in Bulgarian and Turkish folk medicine. Previous studies have reported that hyperoside, isoquercetin, miquelianin, cis-and trans-tiliroside, sinocrassoside D2 and rhodiolgin were detected in A. mollis. Present study is aimed to evaluate in vitro antioxidant effects and cytotoxic activities of A.mollis methanolic extract and its constituents, hyperoside and isoquercetin, on K562 leukemia cell line.
Material and Method: Spectrophotometric MTT assay and NO radical scavenging assay have been used to test the cytotoxic effects and antioxidant activities, respectively.
Result and Discussion: Results showed that A.mollis methanolic extract reduced cell viability of K562 cells at concentrations higher than 0.02 mg/ml whereas the compounds did not exhibit any cytotoxicity at same concentration range. Additionally, significant inhibition on NO radical production was observed for all tested materials.

Anahtar Kelimeler

• Alchemilla mollis
• antileukemic activity
• antioxidant activity
• hyperoside
• isoquercetin

ALCHEMILLA MOLLIS (BUSER) ROTHM. İLE BİLEŞENLERİ HİPEROZİT VE İZOKERSETİNİN SİTOTOKSİK VE ANTİOKSİDAN AKTİVİTELERİ

Öz

Amaç: Alchemilla mollis (Buser) Rothm. (Rosaceae) Avrupa, Kuzey Anadolu, Kafkasya ve Kuzey İran’da geniş yayılış göstermektedir. A.mollis’in toprak üstü kısımlarından elde edilen ticari ilaç "Herba Alchemillae" Bulgar ve Türk halk tıbbında aşırı adet görme ve yara tedavisinin yanı sıra anstrenjan, diüretik, antispazmodik özellikleri için de kullanılmaktadır. Yapılan çalışmalarda A.mollis’te hiperozit, izokersetin, mikuelianin, cis- ve trans-tilirozit, sinokrassozit D2 ve rodiolgin tespit edildiği bildirilmiştir. Bu çalışmanın amacı A.mollis’in metanollü ektresi ile içerdiği bileşikler, hiperozit ve izokersetinin K562 lösemi hücre hattındaki sitotoksik aktiviteleri ile in vitro antioksidan aktivitelerini değerlendirmektir.
Gereç ve Yöntem: Sitotoksik aktiviteyi test etmek için MTT testi, antioksidan aktiviteyi değerlendirmek için NO radikali süpürücü test kullanılmıştır.
Sonuç ve Tartışma: Çalışma sonuçları A.mollis’in metanollü ekstresinin 0.02 mg/ml'den daha yüksek konsantrasyonlarda K562 hücrelerinin hücre canlılığını azaltırken, bileşiklerin aynı konsantrasyon aralığında herhangi bir sitotoksisite sergilemediğini göstermiştir. Ayrıca, test edilen tüm materyallerin NO radikali üretimini önemli ölçüde inhibe ettiği görülmüştür.

Anahtar Kelimeler

• Alchemilla mollis
• antilösemik aktivite
• antioksidan aktivite
• hiperozit
• izokersetin

Kaynakça

1. 1. Renda, G., Özel, A., Barut, B., Korkmaz, B., Soral, M., Kandemir, Ü., Liptaj, T. (2018). Bioassay guided isolation of active compounds from Alchemilla barbatiflora Juz. Records of Natural Products, 12(1), 76-85. [CrossRef]
2. 2. Vitkova, A., Gavrilova, A., Tashev, A. (2011). Alchemilla mollis (Rosaceae)-a critically endangered species in Bulgaria. Phytologia Bulgaria, 17(1), 83-88.
3. 3. Ozbek, H., Bahadir Acikara, O., Keskin, I., Kirmizi, N.I., Ozbilgin, S., Ergene Oz, B., Kurtul, E., Ozrenk, B.C., Tekin, M., Saltan, G. (2017). Evaluation of hepatoprotective, and antidiabetic, activity of Alchemilla mollis. Biomedicine & Pharmacotherapy, 86, 172-176. [CrossRef]
4. 4. Turk, M., Kaya, B., Menemen, Y., Oguztuzun, S. (2011). Apoptotic and necrotic effects of plant extracts belonging to genus Alchemilla L. species on HeLa cells in vitro. Journal of Medicinal Plants Research, 5(18), 4566-4571.
5. 5. Baytop, T. (1999). Türkiye’de Bitkiler ile Tedavi Geçmişte ve Bugün, Nobel Tıp Kitabevleri, İstanbul, p. 370.
6. 6. Sagiroglu, M., Arslanturk, A., Akdemir, Z.K., Turna, M. (2012). An ethnobotanical survey from Hayrat (Trabzon) and Kalkandere (Rize/Turkey). Biological Diversity and Conservation, 5(1), 31-43.
7. 7. Saraç, D.U., Ozkan Z.A., Akbulut S. (2013). Ethnobotanic features of Rize/Turkey province. Biological Diversity and Conservation, 6(3), 57-66.
8. 8. Akbulut, S., Bayramoğlu, M.M. (2014). Reflections of socio-economic and demographic structure of urban and rural on the use of medicinal and aromatic plants: the sample of Trabzon Province. EthnoMedicine, 8(1), 89-100. [CrossRef]

9. 9. Akbulut, S., Ozkan, Z.C. (2014). Traditional usage of some wild plants in Trabzon region (Turkey). Kastamonu University Journal of Forestry Faculty, 14(1), 135-145.
10. 10. Kaval, I., Behcet, L., Cakılcıoğlu, U. (2014). Ethnobotanical study on medicinal plants in Geçitli and its surrounding (Hakkari-Turkey). Journal of Ethnopharmacology, 155, 171-184. [CrossRef]
11. 11. Guzel, Y., Guzelsemme, M., Miski, M. (2015). Ethnobotany of medicinal plants used in Antakya: A multicultural district in Hatay Province of Turkey. Journal of Ethnopharmacognosy, 174, 118-152. [CrossRef]
12. 12. Kalankan, G., Özkan, Z.C., Akbulut, S. (2015). Medicinal and aromatic wild plants and traditional usage of them in Mount Ida (Balıkesir/Turkey). Journal of Applied Biological Sciences, 9(3), 25-33.
13. 13. Mukemre, M., Behcet, L., Cakılcıoglu, U. (2015). Ethnobotanical study on medicinal plants in villages of Çatak (Van-Turkey). Journal of Ethnopharmacognosy, 166, 361-374. [CrossRef]
14. 14. Condrat, D., Szabo, M.R., Crisan, F., Lupea, A.X. (2009). Antioxidant activity of some phanerogam plant extracts. Food Science and Technology Research, 15(1), 95-98. [CrossRef]
15. 15. Makau, J.N., Watanabe, K., Kobayashi N. (2013). Anti-influenza activity of Alchemilla mollis extract: Possible virucidal activity against influenza virus particles. Drug Discoveries & Therapeutics, 7(3), 189-195.
16. 16. Ergene, B., Bahadır Acıkara, Ö., Bakar, F., Saltan, G., Nebioğlu, S. (2010). Antioxidant activity and phytochemical analysis of Alchemilla persica Rothm. Journal of Faculty of Pharmacy of Ankara, 39(2), 145-154. [CrossRef]
17. 17. Küpeli Akkol, E., Demirel, M.A., Bahadır Acıkara, Ö., Süntar, İ., Ergene, B., İlhan, M., Özbilgin, S., Saltan, G., Keleş, H., Tekin, M. (2015). Phytochemical analyses and effects of Alchemilla mollis (Buser) Rothm. and Alchemilla persica Rothm. in rat endometriosis model. Archieves of Gynecoogy and Obstetrics, 292, 619-928. [CrossRef]
18. 18. Marcocci, L., Maguire, J.J., Droyl-Lefaix, M.T., Packer, L. (1994). The nitric oxide scavenging properties of Ginkgo biloba extract. Biochemical and Biophysical Research Communications, 201, 748-755. [CrossRef]
19. 19. Şeker Karatoprak, G., İlgün, S., Koşar, M. (2017). Phenolic composition, anti-inflammatory, antioxidant, and antimicrobial activities of Alchemilla mollis (Buser) Rothm. Chemistry & Biodiversity, 14(9), 1-19. [CrossRef]
20. 20. Perveen, S., Al-Taweel, A.M. (2017). Phenolic compounds from the natural sources and their cytotoxicity. In: Soto-Hernandez, M., Palma-Tenango, M., Garcia-Mateos, M.R. (Eds.), Phenolic Compounds-Natural Sources, ımpertance and Applications, (pp. 29-60). InTechOpen.
21. 21. Soumya, K., Haridas, K.R., James, J., Sameer Kumar, V.B., Edatt, L., Sudheesh, S. (2019). Study of in vitro antioxidantand DNA damage protection activity of a novel luteolin derivative isolated from Terminalia chebula. Journal of Taibah University for Science, 13, 755-763. [CrossRef]
22. 22. Saenglee, S., Jogloy, S., Patanothai, A., Senawong, T. (2016). Cytotoxic effects of peanut phenolic compounds possessing histone deacetylase inhibitory activity on human colon cancer lines. Turkish Journal of Biology, 40(5), 1258-1271. [CrossRef]