ARE THE DISINFECTANTS USED IN HOSPITALS ALSO EFFECTIVE ON BACTERIA THAT CAUSE NOSOCOMIAL INFECTIONS?: A UNIVERSITY HOSPITAL INVESTIGATION

Öz

Objective: Determining the disinfectant activity regularly will result in proper disinfection. In our study, it was aimed to determine the efficacy, effective concentrations and durations of some disinfectants used in Trakya University Hospital against nosocomial bacteria isolated from the same center.

Material and Method: MRSA, VRE, Klebsiella pneumoniae and Acinetobacter baumannii isolates and 10 different disinfectants supplied from the same center were included in the study. Susceptibility tests and Qualitative Suspension Test were applied through the guidelines of CLSI M100-S25 and TS/EN/1040:2005 (formerly TS/EN/1040:1999), respectively. Qualitative Suspension Test was modified by using D/E Neutralizing Broth instead of adding a neutralizing agent.

Result and Discussion: As a result, it was determined that all isolates were susceptible in the application conditions. However, peracetic acid, sodium dichloroisocyanurate, non-ionic active agent and sodium hypochlorite containing disinfectants being used in the hospital were not effective against some strains isolated from the hospital. Even the resistance of a single isolate to the application conditions of a disinfectant may lead to selection and proliferation of the strains and this may cause hospital infections. Our results might provide a reference to indicate the importance of selecting appropriate disinfectants and appropriate conditions for cleaning and disinfection in hospitals.

Anahtar Kelimeler

• Acinetobacter baumannii
• ESBL
• Klebsiella pneumoniae
• MRSA
• Qualitative Suspension Test
• VRE

HASTANEDE KULLANILAN DEZENFEKTANLAR NOZOKOMİYAL ENFEKSİYON ETKENİ BAKTERİLER ÜZERİNE DE ETKİLİ Mİ?: BİR ÜNİVERSİTE HASTANESİ GÖZLEMİ

Öz

Amaç: Dezenfektan aktivitesinin düzenli aralıklarla belirlenmesi, uygun dezenfeksiyonla sonuçlanacaktır. Çalışmamızda Trakya Üniversitesi Hastanesinde kullanılan bazı dezenfektanların aynı merkezden izole edilen nozokomiyal bakterilere karşı etkinlik, etki konsantrasyonları ve sürelerinin belirlenmesi amaçlanmıştır.

Gereç ve Yöntem: MRSA, VRE, Klebsiella pneumoniae ve Acinetobacter baumannii izolatları ile aynı merkezden temin edilen 10 farklı dezenfektan çalışmaya dahil edildi. Dirençli oldukları, CLSI önerileri doğrultusunda yapılan duyarlılık ve doğrulama testleri ile gösterilen izolatlar çalışmaya alınmış ve dezenfektan etkinliği TS/EN/1040:2005 (eski adıyla TS/EN/1040:1999) önerileri doğrultusunda kalitatif süspansiyon testi ile saptanmıştır.

Sonuç ve Tartışma: Sonuç olarak perasetik asit, sodyumdikloroizosiyanurat, non-iyonik aktif madde ve sodium hipoklorit hastaneden izole edilen bazı izolatlar üzerine etkisiz bulunmuştur. Uygulama koşullarında, bir dezenfektanın etki etmediği tek bir izolatın olması bile, bu suşun seçilimine ve çoğalmasına neden olarak hastane enfeksiyonlarına sebep olabilir. Bu nedenle, sonuçlarımızın, hastanelerde uygun dezenfektanların, uygun koşullarda uygulanması ve hastane görülen izolatlar üzerinde de test edilmesi gerektiğini göstermesi açısından önemli olduğu düşünülmektedir.

Anahtar Kelimeler

• Acinetobacter baumannii
• ESBL
• kalitatif süspansiyon testi
• Klebsiella pneumoniae
• MRSA
• VRE

Kaynakça

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