Araştırma Makalesi
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GLİSİN İLE TİYOTRİAZOLİN SABİT KOMBİNASYONUNUN DENEYSEL SEREBRAL İSKEMİ ŞARTLARINDA BEYİN ENERJİ METABOLİZMASI GÖSTERGELERİNE ETKİSİ

Yıl 2018, Cilt: 42 Sayı: 1, 14 - 21, 31.01.2018

Öz

Amaç: Serebral iskemide primer nörokoruma uyarıcı ve inhibitor nörotransmiter sistemleri arasındaki
bozulan dengenin doğal inhibitor yolakların aktivasyonu ile düzeltilmesi yaklaşımına dayanır. Antioksidan bir
madde olan tiyotriazolinin nöro-metabolik serebral koruyucuların terapötik etkilerini arttırdığına ilişkin
kanıtlar bulunmaktadır. Bu nedenle glisin ve tiyotriazolini birlikte içeren yeni bir ilaç kombinasyonu oluşturmak
ilginç olabilir. Bu çalışmada glisinin, ve tiyotriazolin ile kombine kullanımının akut serebrovasküler bozukluk
(ASVB) oluşturulmuş rat modelinde hidrokarbon-enerji prosesleri ve oksidatif metabolizma parametreleri
üzerindeki etkilerinin araştırılması amaçlanmıştır.

Gereç ve Yöntem: ASVB, klasik yöntem olan ana karotis arterlerin simültane ligasyonu ile
gerçekleştirilmiş ve deneylerde 50 erkek Wistar rat kullanılmıştır. Uygulamalara gruplardaki ratlar anesteziden
çıktıktan hemen sonra intraperitonal olarak başlanmış ve 4 gün boyunca devam edilmiştir. Beyin kortekslerinde
adenil nükleotidler, piruvat, laktat, malat ve isositrat düzeyleri, suksinat dehidrogenaz, sitokrom C-oksidaz,
glutamat dekarboksilaz ve GABA-transferaz aktiviteleri biyokimyasal yöntemlerle saptanmıştır.

Sonuç ve Tartışma: Glisin ve tiyotriazolinin birlikte kullanımının, beyin enerji metabolizması
göstergeleri üzerine referans ilaçlar olan pirasetam ve glisinden daha etkili olması, bu kombinasyon üzerinde
araştırmaların devam etmesi gerekliliğini göstermektedir.

Kaynakça

  • Gusev, Е.I., Skvortsova, V.I. (2001). Brain ischemia, Medicine, Moscow, p.328.
  • Mazur, I.А., Chekman, I.S., Belenichev, I.F. (2007). Metabolitotropic drugs, Pechatnyi Dom, Zaporozhye, p.309.
  • Galenko-Yaroshevskiy, P.А., Chekman, I.S., Gorchakova, N.А. (2001). Essays on the pharmacology of metabolic therapy, Medicine, Moscow, p.240.
  • Khizhnyak, А.А., Kursov, S.V. (2003). Participation of excitatory amino acid transmitters in the mechanisms of neurodestruction and perspective methods of pathogenetic correction. Pain Anesthetization and Acute treatment, 1, 43 – 46.
  • Astakhov, А. (2004). Glycised-KMP: just amino acid or multipurpose anti stress medication? Ukraine Drugs, 1, 35 – 36.
  • Gorchakova, N.А., Belenichev, I.F., Mazur, I.А. (2007). Mechanism of antioxidant and antiischemic action of thiotriazoline. Medicine Pharmacy News, 2(206), 11-21.
  • Belenichev, І.F., Gorbachova, S.V., Golovkin, V.V., Bukhtiyarova, N.V. (2006). Influence of the composition “Magnelong”, glycine, emoxipine and piracetam on the development of oxidative stress in the brain of rats with acute cerebrovascular disorders (ischemic stroke). Medical Chemistry, 3, 107-110.
  • Mazur, I.A., Chekman, I. S., Belenichev, I.F., Gorchakova, N.А. (2011). Development of drugs based on fixed combinations with antioxidants - a promising area of modern pharmacology. Pharmacology and Drug Toxicology, 5, 199–200.
  • Kozhemyakin, Y.М., Khromov, О.S., Filonenko, М.А., Sayftedinovna, G.А. (2002). Scientific and methodical recommendations for the maintenance of laboratory animals and work with them. Avitsena Kiev, p.156.
  • Voronina, Т.А., Seredinin, S.B. (2002). Manual on experimental (preclinical) study of new pharmacological substances, Information and analytical portal of the Ministry of Health of the Russian Federation ZAO “Remedium”, p.320.

INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA

Yıl 2018, Cilt: 42 Sayı: 1, 14 - 21, 31.01.2018

Öz

Objective: The perspective direction of primary neuroprotection in cerebral ischemia is the correction
of the imbalance of excitatory and inhibitory neurotransmitter systems by activating natural inhibitory
processes. There is evidence of the ability of anti-oxidant thiotriazoline to potentiate the therapeutic effect of
neuro-metabolic cerebroprotectors. Therefore, it is interesting to create a new combined drug based on glycine
and thiotriazoline. The purpose of this study is to investigate the effect of glycine, as well as its combination
with thiotriazoline, on the parameters of hydrocarbon-energy processes and oxidative metabolism under the
conditions of simulation of acute cerebrovascular disorder (ACVD).


Material and Method: To create ACVD, a classic model consisting of simultaneous ligation of common
carotid arteries was used in 50 Wistar male rats. All drugs were administered intraperitonally for four days
starting with anesthesia recovery of rat groups. The content of adenyl nucleotides, pyruvate, lactate, malate,
isocitrate and activities of succinate dehydrogenase, cytochrome C-oxidase, glutamate decarboxylase, GABAtransferase were determined in the homogenates of brain cortex by biochemical methods.

Result and Discussion: Our results showed that the combination of glycine with thiotriazoline is better
than such reference drugs like pyracetam and glycine, according to degree of effect on the indicators of energy
metabolism of the brain, indicating the relevance of further study of the proposed combination. 

Kaynakça

  • Gusev, Е.I., Skvortsova, V.I. (2001). Brain ischemia, Medicine, Moscow, p.328.
  • Mazur, I.А., Chekman, I.S., Belenichev, I.F. (2007). Metabolitotropic drugs, Pechatnyi Dom, Zaporozhye, p.309.
  • Galenko-Yaroshevskiy, P.А., Chekman, I.S., Gorchakova, N.А. (2001). Essays on the pharmacology of metabolic therapy, Medicine, Moscow, p.240.
  • Khizhnyak, А.А., Kursov, S.V. (2003). Participation of excitatory amino acid transmitters in the mechanisms of neurodestruction and perspective methods of pathogenetic correction. Pain Anesthetization and Acute treatment, 1, 43 – 46.
  • Astakhov, А. (2004). Glycised-KMP: just amino acid or multipurpose anti stress medication? Ukraine Drugs, 1, 35 – 36.
  • Gorchakova, N.А., Belenichev, I.F., Mazur, I.А. (2007). Mechanism of antioxidant and antiischemic action of thiotriazoline. Medicine Pharmacy News, 2(206), 11-21.
  • Belenichev, І.F., Gorbachova, S.V., Golovkin, V.V., Bukhtiyarova, N.V. (2006). Influence of the composition “Magnelong”, glycine, emoxipine and piracetam on the development of oxidative stress in the brain of rats with acute cerebrovascular disorders (ischemic stroke). Medical Chemistry, 3, 107-110.
  • Mazur, I.A., Chekman, I. S., Belenichev, I.F., Gorchakova, N.А. (2011). Development of drugs based on fixed combinations with antioxidants - a promising area of modern pharmacology. Pharmacology and Drug Toxicology, 5, 199–200.
  • Kozhemyakin, Y.М., Khromov, О.S., Filonenko, М.А., Sayftedinovna, G.А. (2002). Scientific and methodical recommendations for the maintenance of laboratory animals and work with them. Avitsena Kiev, p.156.
  • Voronina, Т.А., Seredinin, S.B. (2002). Manual on experimental (preclinical) study of new pharmacological substances, Information and analytical portal of the Ministry of Health of the Russian Federation ZAO “Remedium”, p.320.
Toplam 10 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Lyudmila Kucherenko Bu kişi benim

İgor Belenıchev Bu kişi benim

İvan Mazur Bu kişi benim

Olga Khromylova Bu kişi benim

Natalia Parnıuk Bu kişi benim

Yayımlanma Tarihi 31 Ocak 2018
Gönderilme Tarihi 20 Aralık 2017
Yayımlandığı Sayı Yıl 2018 Cilt: 42 Sayı: 1

Kaynak Göster

APA Kucherenko, L., Belenıchev, İ., Mazur, İ., Khromylova, O., vd. (2018). INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA. Journal of Faculty of Pharmacy of Ankara University, 42(1), 14-21.
AMA Kucherenko L, Belenıchev İ, Mazur İ, Khromylova O, Parnıuk N. INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA. Ankara Ecz. Fak. Derg. Ocak 2018;42(1):14-21.
Chicago Kucherenko, Lyudmila, İgor Belenıchev, İvan Mazur, Olga Khromylova, ve Natalia Parnıuk. “INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA”. Journal of Faculty of Pharmacy of Ankara University 42, sy. 1 (Ocak 2018): 14-21.
EndNote Kucherenko L, Belenıchev İ, Mazur İ, Khromylova O, Parnıuk N (01 Ocak 2018) INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA. Journal of Faculty of Pharmacy of Ankara University 42 1 14–21.
IEEE L. Kucherenko, İ. Belenıchev, İ. Mazur, O. Khromylova, ve N. Parnıuk, “INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA”, Ankara Ecz. Fak. Derg., c. 42, sy. 1, ss. 14–21, 2018.
ISNAD Kucherenko, Lyudmila vd. “INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA”. Journal of Faculty of Pharmacy of Ankara University 42/1 (Ocak 2018), 14-21.
JAMA Kucherenko L, Belenıchev İ, Mazur İ, Khromylova O, Parnıuk N. INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA. Ankara Ecz. Fak. Derg. 2018;42:14–21.
MLA Kucherenko, Lyudmila vd. “INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA”. Journal of Faculty of Pharmacy of Ankara University, c. 42, sy. 1, 2018, ss. 14-21.
Vancouver Kucherenko L, Belenıchev İ, Mazur İ, Khromylova O, Parnıuk N. INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA. Ankara Ecz. Fak. Derg. 2018;42(1):14-21.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.