ÇOK AMAÇLI DÖRT KONTAKT LENS BAKIM SOLÜSYONUNUN ANTİMİKROBİYAL ETKİNLİKLERİ

Yıl 2018, Cilt: 42 Sayı: 2, 15 - 22, 31.05.2018

Mujde Eryılmaz Banu Kaskatepe Merve Eylul Kıymacı Duygu Sımsek Hilal Basak Erol

Öz

Amaç: Kontakt lens ile ilişkili enfeksiyonların önlenmesi ve göz sağlığının korunması için kontakt lenslerin uygun bakımı çok önemlidir. Çok amaçlı solüsyonlar, yumuşak kontakt lenslerin temizlenmesi, durulanması, dezenfekte edilmesi ve saklanması gibi çeşitli amaçlar için kullanılmaktadır.

Gereç ve Yöntem: Çalışmamızda ticari olarak satılan çok amaçlı dört kontakt lens bakım solüsyonunun (ÇAKLBS) etkinlikleri araştırılmıştır. Bu amaçla, beş bakteri ve bir maya olmak üzere altı mikroorganizma kullanılmış ve dört adet çok amaçlı kontakt lens bakım solüsyonu; ReNu® MultiPlus, Opti-Free® Express, All in One Light, Biotrue ™ değerlendirilmiştir. Test mikroorganizması olarak S. epidermidis ATCC 35948 (biyofilm oluşturan), S. epidermidis ATCC 12228 (biyofilm oluşturmayan), Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 25923, S. aureus ATCC 43300 (metisilin dirençli) ve Candida albicans ATCC 10231 kullanılmıştır. Kantitatif süspansiyon testi yapılmış ve sonuçlar EN ISO 14729 bağımsız test birincil kriterlerine göre değerlendirilmiştir. 6 saatlik temas süresi sonunda ortalama log indirgeme değerlerine göre, tüm ÇAKLBS’ ler test mikroorganizmalarına karşı etkili bulunmuştur.

Sonuç ve Tartışma: Piyasada çeşitli ticari ÇAKLBS tipleri bulunmaktadır. Bu ürünlerin antimikrobiyal etkinlikleri, içeriklerinde bulunan antimikrobiyal maddelerin tipine ve konsantrasyonlarına bağlıdır. Bu çalışmada, tüm ÇAKLBS' ler, üreticinin tavsiye ettiği temas süresinde test mikroorganizmalarına karşı etkili bulunmuştur.

Anahtar Kelimeler

Antimikrobiyal ajanlar, biyofilm, kontakt lens solüsyonları, Staphylococcus aureus, Staphylococcus epidermidis

ANTIMICROBIAL EFFICACIES OF FOUR MULTI-PURPOSE CONTACT LENS CARE SOLUTIONS

Öz

Objective: Proper care of contact lens is important for preventing contact lens related infections and for maintaining eye health. Multi-purpose solutions are used for various purposes such as cleaning, rinsing, disinfecting, and storing soft contact lenses.

Material and Method: In our work, the activities of four commercially available multi-purpose contact lens care solutions (MPCLCSs) were investigated. For this purpose, six microorganisms, including five strains of bacteria and one of yeast, were used and four Multi-Purpose Solutions including ReNu® MultiPlus, Opti-Free® Express, All In One Light, BiotrueTM were evaluated. S. epidermidis ATCC 35948 (biofilm forming), S. epidermidis ATCC 12228 (non-biofilm forming), Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 25923, S. aureus ATCC 43300 (methicillin resistant) and Candida albicans ATCC 10231 were used as test microorganisms. The quantitative suspension test was performed and results were evaluated in accordance with EN ISO 14729 Stand-alone test primary criteria. According to the mean log reduction values for 6 h contact time, all the MPCLCSs were found effective against test microorganisms.

Result and Discussion: There are various types of commercial MPCLCSs available on the market. The antimicrobial efficacies of these products depend on the type and concentrations of the antimicrobial agents present in their content. In this study, all the MPCLCSs were found effective against test microorganisms at manufacturer's recommended contact time.

Anahtar Kelimeler

Antimicrobial agents, biofilm, contact lens solutions, Staphylococcus aureus, Staphylococcus epidermidis

Kaynakça

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