Yıl 2020, Cilt 44 , Sayı 1, Sayfalar 50 - 60 2020-01-31

Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts
YEŞİL ÇAY VE SİYAH ÇAY EKSTRELERİNDEKİ GALLİK ASİT, EGCG İÇERİĞİ VE ANTİRADİKAL AKTİVİTESİNİN DEĞERLENDİRİLMESİ

Asli CAN AGCA [1] , Kadir BATÇIOĞLU [2] , Engin ŞARER [3]


Objective: Tea is very well known and consumed beverage worldwide also cultivated widely. It is one of phytonutrients that has a protective role associated with its antioxidant activity. The aim of this article is to study the gallic acid, epigallocatechin gallate contents and antiradical activity of green tea and  black tea extracts from Camellia sinensis cultivated in North Anatolia.

Material and Method: Gallic acid and epigallocatechin gallate contents were investigated in ethanol, methanol and water extracts of green tea and black tea by HPLC analysis and the antiradical activities were also examined for scavenging effect on DPPH and ABTS free radicals.

Result and Discussion: In total 6 extracts, gallic acid contents were determined in the range of 0.052-1.341 mg/100 ml and the value of EGCG (epigallocatechin gallate) were found between 0-19.54 mg/100ml. Water extract of green tea exhibited the best antiradical activity on both DPPH and ABTS radicals. Green tea could be evaluated as a good candidate for health prevention but it should be noted that the harvesting method and manufacturing process, optimum conditions on brewing time, the solvent used, chopping grade of tea leaves should also be taken into consideration during formulating both phytonutrient and pharmaceutical grade products. 

Amaç: Çay, dünyada çok iyi bilinen ve tüketilen bir içecek olup antioksidan aktivitesine bağlı koruyucu etkileri bilinen önemli bir fitobesindir. Bu çalışmanın amacı, Kuzey Anadolu kaynaklı Camellia sinensis bitkisinden elde edilen yeşil çay ve siyah çaydan hareketle elde edilen ekstrelerdeki gallik asit ve epigallokateşin gallat miktarı ile ekstrelerin antiradikal aktivitesinin tespit edilmesidir.

Gereç ve Yöntem: Yeşil çay ve siyah çayın etanol, metanol ve sulu ekstrelerindeki gallik asit ve epigallokateşin gallat miktar tayini YPSK yöntemi ile tespit edilmiş ve ekstrelerin antiradikal aktivite tayini için DPPH ve ABTS yöntemleri kullanılmıştır.

Sonuç ve Tartışma: Toplam 6 ekstredeki gallik asit içeriği 0.052-1.341 mg/100 ml aralığında bulunmuş ve EGCG (epigallokateşin gallat) için bu değer aralığı 0-19.54 mg/100ml olarak tespit edilmiştir. Hem DPPH hem de ABTS radikali üzerinde en iyi etkiyi yeşil çayın sulu ekstresinin sağladığı belirlenmiştir. Yeşil çayın, koruyucu sağlıkta değerlendirilebilecek iyi bir aday olduğu ancak fitobesin ve farmasötik kalitede ürünün formülasyon çalışmalarında, bitkinin hasat yöntemi, bitkiden hareketle çay üretim prosesi, optimum karıştırma zamanı, kullanılan çözücü ve yaprakların parçalanma derecesinin kayıt altına alınması gerekmektedir.

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Birincil Dil en
Konular Farmakoloji ve Eczacılık
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-5710-3479
Yazar: Asli CAN AGCA (Sorumlu Yazar)
Kurum: ANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0001-6663-2287
Yazar: Kadir BATÇIOĞLU
Kurum: İNÖNÜ ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0001-6681-2309
Yazar: Engin ŞARER
Kurum: ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 31 Ocak 2020

Bibtex @araştırma makalesi { jfpanu644407, journal = {Journal of Faculty of Pharmacy of Ankara University}, issn = {1015-3918}, eissn = {2564-6524}, address = {Ankara University Faculty of Pharmacy Degol Str. TR-06100 Tandogan/Ankra/TURKEY}, publisher = {Ankara Üniversitesi}, year = {2020}, volume = {44}, pages = {50 - 60}, doi = {10.33483/jfpau.644407}, title = {Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts}, key = {cite}, author = {CAN AGCA, Asli and BATÇIOĞLU, Kadir and ŞARER, Engin} }
APA CAN AGCA, A , BATÇIOĞLU, K , ŞARER, E . (2020). Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts. Journal of Faculty of Pharmacy of Ankara University , 44 (1) , 50-60 . DOI: 10.33483/jfpau.644407
MLA CAN AGCA, A , BATÇIOĞLU, K , ŞARER, E . "Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts". Journal of Faculty of Pharmacy of Ankara University 44 (2020 ): 50-60 <https://dergipark.org.tr/tr/pub/jfpanu/issue/52196/644407>
Chicago CAN AGCA, A , BATÇIOĞLU, K , ŞARER, E . "Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts". Journal of Faculty of Pharmacy of Ankara University 44 (2020 ): 50-60
RIS TY - JOUR T1 - Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts AU - Asli CAN AGCA , Kadir BATÇIOĞLU , Engin ŞARER Y1 - 2020 PY - 2020 N1 - doi: 10.33483/jfpau.644407 DO - 10.33483/jfpau.644407 T2 - Journal of Faculty of Pharmacy of Ankara University JF - Journal JO - JOR SP - 50 EP - 60 VL - 44 IS - 1 SN - 1015-3918-2564-6524 M3 - doi: 10.33483/jfpau.644407 UR - https://doi.org/10.33483/jfpau.644407 Y2 - 2019 ER -
EndNote %0 Ankara Üniversitesi Eczacılık Fakültesi Dergisi Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts %A Asli CAN AGCA , Kadir BATÇIOĞLU , Engin ŞARER %T Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts %D 2020 %J Journal of Faculty of Pharmacy of Ankara University %P 1015-3918-2564-6524 %V 44 %N 1 %R doi: 10.33483/jfpau.644407 %U 10.33483/jfpau.644407
ISNAD CAN AGCA, Asli , BATÇIOĞLU, Kadir , ŞARER, Engin . "Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts". Journal of Faculty of Pharmacy of Ankara University 44 / 1 (Ocak 2020): 50-60 . https://doi.org/10.33483/jfpau.644407
AMA CAN AGCA A , BATÇIOĞLU K , ŞARER E . Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts. Ankara Ecz. Fak. Derg.. 2020; 44(1): 50-60.
Vancouver CAN AGCA A , BATÇIOĞLU K , ŞARER E . Evaluation on Gallic Acid, EGCG Contents and Antiradical Activity of Green Tea and Black Tea Extracts. Journal of Faculty of Pharmacy of Ankara University. 2020; 44(1): 60-50.