A PRELIMINARY STUDY ON THE ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF OPOPANAX HISPIDUS

Yıl 2021, Cilt: 45 Sayı: 3, 577 - 585, 27.09.2021

Safa Gümüşok , Sezen Yılmaz Sarıaltın Tülay Çoban , Ceyda Sibel Kılıç

https://doi.org/10.33483/jfpau.972652

Öz

Objective: Opopanax W. Koch genus is included in Apiaceae family. The genus can be distinguishable with its compound basal leaves, glabrous fruits, yellow flowers, tall stem with glochidate and stellate hairs. It is known with the name “Hercules’ all-heal” throughout the world. It was used for the treatment of epilepsy, infertility in women, hemorrhoids and paralysis traditionally. This present research was designed to assess in vitro antioxidant and anti-inflammatory activity of Opopanax hispidus (Friv.) Griseb.

Material and Method: Methanol extracts were prepared from the aerial parts and flowers of the plants. Their antioxidant activities were analyzed through a number of chemical assays: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-l-picrylhydrazyl (DPPH) free radical scavenging assays. Anti-inflammatory activities of the extracts were compared and evaluated using human red blood cell membrane stability testing.

Result and Discussion: Aerial parts exhibited stronger ABTS and DPPH free radical scavenging activities than flowers. Howver, flowers were found to be more active than aerial parts in terms of anti-inflammatory effects (IC50=3,32 mg/ml and 4,75 mg/ml; respectively). These data suggest that flowers and aerial parts of Opopanax hispidus exhibited antioxidant and anti-inflammatory effects. More research studies are necessary to determine the active ingredients in charge of this activity.

Anahtar Kelimeler

Anti-inflammatory, Anti-oxidant, ABTS, DPPH, Opopanax hispidus

OPOPANAX HISPIDUS’UN ANTİOKSİDAN VE ANTİ-İNFLAMATUAR AKTİVİTELERİ ÜZERİNE BİR ÖN ÇALIŞMA

Öz

Amaç: Opopanax W. Koch cinsi, Apiaceae familyasında yer alır. Cins, bileşik bazal yaprakları, tüysüz meyveleri, sarı çiçekleri, gloşidat ve yıldızsı tüyleri olan uzun gövdesi ile ayırt edilebilir. Dünya çapında “Herkül'ün her şeyi iyileştireni” adıyla bilinir. Halk arasında epilepsi, kadınlarda kısırlık, hemoroid ve felç tedavisi için kullanılır. Bu araştırma, Opopanax hispidus (Friv.) Griseb'in in vitro antioksidan ve anti-inflamatuar aktivitesini değerlendirmek için tasarlanmıştır.

Gereç ve Yöntem: Bitkinin toprak üstü kısımlarından ve çiçeklerinden metanol ekstreleri hazırlandı. Antioksidan aktiviteleri bir dizi kimyasal deneyle analiz edildi: 2,2'-azino-bis-3-etilbenzotiazolin- 6-sülfonik asit (ABTS) ve 2,2-difenil-l-pikrilhidrazil (DPPH) serbest radikal süpürme deneyleri. Ekstrelerin anti-inflamatuar aktiviteleri insan kırmızı kan hücresi membran stabilite testi kullanılarak karşılaştırıldı ve değerlendirildi.

Sonuç ve Tartışma: Toprak üstü kısımları, çiçeklerden daha güçlü ABTS ve DPPH serbest radikal temizleme aktiviteleri sergiledi. Ancak anti-inflamatuvar etki açısından çiçeklerin toprak üstü kısımlardan daha aktif olduğu bulundu (IC50=3,32 mg/ml ve 4,75 mg/ml, sırasıyla). Bu veriler, Opopanax hispidus'un çiçek ve toprak üstü kısımlarının antioksidan ve anti-inflamatuar etkiler sergilediğini göstermektedir. Bu aktiviteden sorumlu aktif içerikleri belirlemek için daha fazla araştırma çalışması gereklidir.

Anahtar Kelimeler

ABTS, anti-inflamatuvar, anti-oksidan, DPPH, Opopanax hispidus

Kaynakça

• 1. Downie, S.R., Katz-Downie D.S.,Watson M.F. (2000). “A phylogeny of the flowering plant family Apiaceae based on chloroplast DNA rpl16 and rpoC1 intron sequences: towards a suprageneric classification of subfamily Apioideae”, American Journal of Botany, 87, 273–292.
• 2. Olle, M., Bender I. (2010). ”The content of oils in umbelliferous crops and its formation”, Agronomy Research, 8, 687–696.
• 3. https://www.etymonline.com/search?q=opos - erişim tarihi 13.01.2019 https://www.etymonline.com/word/panacea - erişim tarihi 13.01.2019
• 4. Sahranavard, S., Ghafari, S., Mosaddegh, M. (2014). “Medicinal plants used in Iranian traditional medicine to treat epilepsy”, Seizure, 23, 328-332.
• 5. Adams, M., Schneider, S., Kluge, M., Kessler, M., Hamburger, M. (2012). “Epilepsy in the Renaissance: A survey of remedies from 16th and 17th century German herbals”, Journal of Ethnopharmacology, 143, 1-13.
• 6. Lev, E., Amar, Z. (2008). “Fossils of practical medical knowledge from medieval Cairo”, Journal of Ethnopharmacology, 119, 24-40.
• 7. Javadi, B., Iranshahy, M., Emami, S.A. (2015). “Anticancer Plants in Islamic Traditional Medicine, Complementary Therapies for the Body, Mind and Soul”, Editör: Marcelo Saad, InTech, DOI: 10.5772/61111
• 8. Farzaei, M.H., Farzaei, F., Abdollahi, M., Abbasabadi, Z., Abdolghaffari, A.H., Mehraban, B. (2016). “A mechanistic review on medicinal plants used for rheumatoid arthritis in traditional Persian medicine”, Journal of Pharmacy and Pharmacology, 68, 1233-1248.
• 9. Ahıskalı, M., Arı, Ç., Selvi, S. (2012). “Edible wild plants and their consumption during winter in a rural village on Kazdağı (Mount Ida)”, Bocconea, 24, 195-198.
• 10. Özgen, U., Kaya, Y., Houghton, P. (2010) Folk medicines in the villages of Ilıca District (Erzurum, Turkey), Turkish Journal of Biology, 36, 93-106
• 11. Tuzlacı, E., Erol, M.K. (1999). “Turkish folk medicinal plants. Part II: Eğirdir (Isparta)”, Fitoterapia, 70, 593-610.
• 12. Sevgi, E., Kızılarslan, Ç. (2013). “Bir İsim Çok Bitki - Mayasıl Otu”, Avrasya Terim Dergisi, 1, 17-29.
• 13. Valiakos, E., Marselos, M., Sakellaridis, N., Constantinidis, Th., Skaltsa, H. (2015). “Ethnopharmacological approach to the herbal medicines of the “Antidotes” in Nikolaos Myrepsos׳ Dynameron”, Journal of Ethnopharmacology, 163, 68-82.
• 14. Amiri, M.S., Joharchi, M.R. (2015). “Ethnobotanical knowledge of Apiaceae family in Iran: A review”, Avicenna Journal of Phytomedicine, 6, 621-635
• 15. Abolhasanzadeh, Z., Ashrafib, H., Badra, P., Azadia, A. (2017). “Traditional neurotherapeutics approach intended for direct nose to brain delivery”, Journal of Ethnopharmacology, 209, 116-123.
• 16. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. (1999).Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. ;26(9-10):1231-7.
• 17. Blois MS.(1958). Antioxidant determination by the use of a stable free radical. Nature; 181: 1199–1200.
• 18. Anosike CA, Obidoa O, Ezeanyika LU. (2012). Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). Daru. Nov 14;20(1):76. doi: 10.1186/2008-2231-20-76.
• 19. Yalçın CÖ, Yılmaz Sarıaltın S, Çiçek Polat D. (2020). Quantification of phenolic and flavonoid contents and some biological activities of Ornithogalum sigmoideum Freyn & Sint. J Res Pharm. 24(4): 487-496.
• 20. Alpınar, K., Özyürek, M., Kolak, U., Güçlü, K., Aras, Ç., Altun, M., Çelik, S. E., Berker, K. I., Bektaşoğlu, B., Apak, R. (2008) Antioxidant Capacities of Some Food Plants Wildly Grown in Ayvalik of Turkey, Food Science and Technology Research, 15, 59-64.
• 21. Matejić, J., Džamić, A., Mihajilov-Krstev, T., Ranđelović, V., & Marin, P. (2015). Antioxidant and antimicrobial potential of Opopanax hispidus (Apiaceae) extracts. Lekovite sirovine, (35), 141-150.
• 22. Sarıkürkçü, C., Targan, Ş., Özer, M. S., Tepe, B. (2016) Fatty acid composition, enzyme inhibitory, and antioxidant activities of the ethanol extracts of selected wild edible plants consumed as vegetables in the Aegean region of Turkey, International Journal of Food Properties, 20, 560-572.
• 23. Sarialtin, S. Y., Coban, T. (2018). An Overview on the Role of Macular Xanthophylls in Ocular Diseases. Records of natural products, 12(2).
• 24. Okur, M. E., Özbek, H., Polat, D. Ç., Yılmaz, S., & Arslan, R. (2018). Hypoglycemic activity of Capparis ovata desf. var. palaestina zoh. methanol extract. Brazilian Journal of Pharmaceutical Sciences, 54(3).
• 25. Samuelsson, G., Bohlin, L. (Eds.), 2009, Drugs of Natural Origin – A Treatise of Pharmacognosy, Swedish Pharmaceutical Society, Swedish Pharmaceutical Pressi P.O Bıx. 1136, S-111, 81 Stockholm, Sweden
• 26. Çiçek Polat, D, YILMAZ SARIALTIN, S, ÇOBAN, T, COŞKUN, M. (2019). Comparison of Walnut (Juglans regia L.) and Olive (Olea europaea L.) Leaves in Terms of Antioxidant and Anti-inflammatory Activity. Adıyaman University Journal of Science, 9 (2), 242-251.