DETERMINATION OF UREAPLASMA UREALYTICUM AND ANTIBIOTIC SUSCEPTIBILITY IN SEXUALLY ACTIVE WOMEN WITH DIFFERENT METHODS

Yıl 2022, Cilt: 46 Sayı: 1, 103 - 113, 29.01.2022

Şükran Öztürk , Sulhiye Yıldız

https://doi.org/10.33483/jfpau.1003224

Öz

Objective: In this study, the presence of Ureaplasma urealyticum (U.urealyticum) in vaginal and urine samples taken from sexually active women was investigated and antibiotic susceptibilities were determined with culturing bacteria and using kits.

Material and Method: The vaginal and urine samples taken from 110 women who applied to Başkent University Ankara Gynecology and Obstetrics Clinic were included in this study. U. urealyticum was investigated by the culture method with using two different test kits [Mycoplasma IES (IES-Autobio, China) and Mycoplasma IST2 (IST2) Biomereux, France] and Mycoplasma Agar. Antibiotic susceptibilities of isolates were also determined.

Result and Discussion: We compared the results of (IES) and (IST2) used for U. urealyticum detection in 220 clinical samples. U. urealyticum was found to be positive in 82 (74,5%) by classical culture method. U. urealyticum was detected at a rate of 46.8% with the IST 2 and 53.6% with the IES. As a result of the comparison of culture method and commercial kits,it was determined that the IES kit provides a fast and accurate identification in the detection of U.urealyticum,a fast and reliable result in the detection of antibiotic resistance.A significant difference was found in the detection rate of U. urealyticum of the IES compared to the other commercial kit.It was observed that the detection rate of U. urealyticum with the kit from vaginal were significantly higher than urine samples.

Anahtar Kelimeler

Antibiotic susceptibility, commercial kit, culture, U. urealyticum

CİNSEL YÖNDEN AKTİF KADINLARDA UREAPLASMA UREALYTİCUM VE ANTİBİYOTİK DUYARLILIĞININ FARKLI YÖNTEMLERLE BELİRLENMESİ

Öz

Amaç: Bu çalışmada, cinsel yönden aktif kadınlardan alınan vajinal ve idrar örneklerinde Ureaplasma urealyticum (U.urealyticum) varlığı, kültür ve kitlerle araştırılmış, ticari kitler kullanılarak da antibiyotik duyarlılıkları belirlenmiştir.

Gereç ve Yöntem: Başkent Üniversitesi Ankara Kadın Hastalıkları ve Doğum Kliniği'ne başvuran 110 kadından alınan vajinal ve idrar örnekleri ile çalışma gerçekleştirilmiştir. U. urealyticum’un Mycoplasma IES (IES-Autobio, China) ve Mycoplasma IST2 (IST2- Biomereux, Fransa) olarak bilinen iki farklı test kiti ile tespiti yapılmış ve antibiyotik duyarlılıkları belirlenmiştir. Ayrıca Mycoplasma Agar kullanılarak kültür yöntemiyle de varlığı araştırılmıştır.

Sonuç ve Tartışma: U. urealyticum tespiti için 220 klinik örnek kullanılmıştır. IES ve IST2 ticari kitleri kullanılarak yapılan tespit sonuçları ile Mycoplasma Agar besiyeri kullanılarak klasik kültür yöntemi ile yapılan çalışma sonuçları karşılaştırılmıştır. Buna göre; kültür yöntemiyle 82 (%74,5) hastada U. urealyticum pozitif bulunmuş olup, U.urealyticum, IST 2 ile %46,8, IES ile (%53,6) oranında tespit edilmiştir. Kültür yöntemi ile ticari kitlerin karşılaştırılması sonucunda, IES kitinin U. urealyticum tespitinde ve antibiyotik duyarlılıklarının saptanmasında hızlı ve doğru bir tanımlama sağladığı sonucuna varılmıştır. IES kitinin U. urealyticum tespit oranında diğer ticari kite göre anlamlı derecede iyi tespit yaptığı ortaya konmuş olup, vajinal örneklerden U. urealyticum tespit oranının, idrar örneklerinden anlamlı derecede yüksek olduğu görülmüştür.

Anahtar Kelimeler

Antibiyotik duyarlılığı, kültür, ticari kit, U. urealyticum

Kaynakça

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