TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI

Yıl 2022, Cilt: 46 Sayı: 1, 48 - 61, 29.01.2022

Hande Yüce , Neşe Başak Türkmen , Dilan Aşkın Özek , Songül Ünüvar

https://doi.org/10.33483/jfpau.995491

Öz

Amaç: Bu çalışmanın amacı, taurin, kapsaisin, melatonin ve beta karoten gibi antioksidan maddelerin insan meme kanseri ve sağlıklı fibroblast hücreleri üzerindeki antiproliferatif, antimigrasyon ve antioksidan aktivitelerini değerlendirmektir.
Gereç ve Yöntem: Bu çalışmada, taurin, kapsaisin, melatonin ve beta karoten L929 sağlıklı fibroblast hücrelerine ve MCF-7 meme kanseri hücrelerine uygulandı. Bileşiklerin antiproliferatif etkisi, MTS [3-(4,5-dimetiltiazol-2-il)-5-(3-karboksimetoksifenil)-2-(4sülfofenil)-2H-tetrazolyum] testi ile 24. ve 48. saatte belirlendi. Hücreler, Dulbecco'nun Modifiye Eagles Ortamı (DMEM) içinde kültürlendi ve ardışık iki gün boyunca farklı konsantrasyonlarla işleme tabi tutuldu. Bileşiklerin hücre göçü üzerindeki etkisi, yara iyileşme deneyi kullanılarak 24. saatte değerlendirildi. Yara iyileşme testi, hücrelerin in vitro metastatik kabiliyetini ölçmek için kullanıldı. Total antioksidan seviyeleri (TAS) ve total oksidan seviyeleri (TOS) ticari kitler kullanılarak belirlendi.
Sonuç ve Tartışma: Tüm bileşikler sağlıklı hücrelere kıyasla, malign hücrelerde hücre canlılığını konsantrasyona ve zamana bağlı bir şekilde azaltmıştır. Yara kapanma alanının sonuçları, bileşiklerle tedavinin, yara kapanmasını önemli ölçüde hızlandıran hücresel göçü iyileştirdiğini göstermiştir. Sonuçlar, tüm bileşiklerin, 48 saatte MCF-7 hücre hatlarının göç kabiliyetini belirgin şekilde inhibe ettiğini gösterdi. Tüm bileşikler antioksidan etki göstermekle birlikte, MCF-7 hücreleri üzerine hemen hemen tüm dozlarda antioksidan etki gösteren bileşiğin beta karoten olduğu gözlenmiştir.

Anahtar Kelimeler

Beta karoten, kapsaisin, melatonin, meme kanseri, taurin

THE INVESTIGATION OF THE ANTIPROLIFERATIVE, ANTIMIGRATION AND ANTIOXIDANT EFFECTS OF TAURINE, CAPSAICIN, MELATONIN AND BETA CAROTEN ON L929 HEALTHY CELLS AND MCF-7 BREAST CANCER CELLS

Öz

Objective: The aim of this study was to evaluate the antiproliferative, antimigration and antioxidant activities of antioxidant substances such as taurine, capsaicin, melatonin and beta carotene on human breast cancer cells.
Material and Method: In this study, taurine, capsaicin, melatonin and beta carotene L929 were applied to healthy fibroblast cells and MCF-7 breast cancer cells. The antiproliferative effect of the compounds was determined by the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulphophenyl)-2H-tetrazolium] test at 24 and 48 hours. Cells were cultured in Dulbecco's Modified Eagles Medium (DMEM) and treated with different concentrations for two consecutive days. The effect of compounds on cell migration was evaluated at 24 hours using the wound healing assay. The wound healing test was used to measure the in vitro metastatic ability of cells. Total antioxidant levels (TAS) and total oxidant levels (TOS) were determined using commercial kits.
Result and Discussion: All compounds decreased cell viability in malignant cells in a concentration- and time-dependent manner compared to healthy cells. The results of the wound closure area showed that treatment with the compounds improved cellular migration, which significantly accelerated wound closure. The results showed that all compounds markedly inhibited the migratory ability of MCF-7 cell lines at 48 hours. Although all compounds showed antioxidant effects, beta carotene was observed to have antioxidant effects on MCF-7 cells at almost all doses.

Anahtar Kelimeler

Beta carotene, breast cancer, capsaicin, melatonin, taurine

Kaynakça

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