STAPHYLOCOCCUS AUREUS'UN KATI LİPİD NANOPARTİKÜLLER İLE ETKİLEŞİMİ

Yıl 2022, Cilt: 46 Sayı: 2, 322 - 329, 29.05.2022

Merve Eylül Kıymacı

https://doi.org/10.33483/jfpau.1063296

Öz

Amaç: Bakterilerin mevcut antibiyotiklere direnç geliştirme yeteneği, yeni antimikrobiyallerin ya da antimikrobiyal formülasyonların araştırılmasının aciliyetini ortaya koymaktadır. İlaç taşıyıcı sistemler arasında, katı lipid nanopartiküller, hedeflenen ilaç uygulaması için avantajlara sahip, çözüm odaklı sistemler olarak kabul edilir.

Gereç ve Yöntem: Bu çalışmada, antibiyotik iyileştirme çalışmaları için bir ön çalışma olması amacıyla floresein yüklenmiş SLN'lerin Staphylococcus aureus ATCC 29213'e bakteri alımı akış sitometrisi yöntemiyle araştırıldı.

Sonuç ve Tartışma: Floresein-SLN'lerin ~%60'ının 1 saat içinde S. aureus ATCC 29213 hücrelerine alındığı belirlendi. Sonuçların gelecekte antibiyotiklerle yapılacak çalışmalar için umut verici olduğu bulundu.

Anahtar Kelimeler

Akış sitometresi, bakteriyel alım, katı lipid nanopartikül, Staphylococcus aureus

INTERACTION OF STAPHYLOCOCCUS AUREUS WITH SOLID LIPID NANOPARTICLES

Öz

Objective: The ability of bacteria to develop resistance to existing antibiotics has made the search for new antimicrobials or antimicrobial formulations a matter of urgency. Among drug delivery systems, solid lipid nanoparticles are considered solution-oriented systems with advantages for targeted drug delivery.

Material and Method: In this study, the bacterial uptake of SLNs encapsulated with fluorescein into Staphylococcus aureus ATCC 29213 was investigated by a flow cytometry method to be a preliminary study for antibiotic improvement studies.

Result and Discussion: It was determined that ~60% of fluorescein-SLNs were taken into S. aureus ATCC 29213 cells within 1h. The results were found to be promising for future studies with antibiotics.

Anahtar Kelimeler

Bacterial uptake, flow cytometry, Staphylococcus aureus, solid lipid nanoparticle

Kaynakça

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