SİPROFLOKSASİN TAYİNİ İÇİN NANOPARTİKÜL TEMELLİ DUYARLI SPEKTROFOTOMETRİK YÖNTEM GELİŞTİRİLMESİ

Yıl 2023, Cilt: 47 Sayı: 1, 95 - 103, 20.01.2023

Hakan Balcı Zehra Özden Erdoğan Alperen Özdemir

https://doi.org/10.33483/jfpau.1160946

Öz

Amaç: Siprofloksasin, pek çok bakteriyel enfeksiyon ile savaşta kullanılan bir florokinolon grubu antibiyotiktir. Bu çalışmanın amacı, siprofloksasinin spektrofotometrik tayini için nanopartikül temelli hızlı ve duyarlı bir yöntem geliştirmektir.

Gereç ve Yöntem: Yöntem geliştirilmesinde metal nanopartikül olarak AgNP kullanıldı. Siprofloksasinin spektrofotometrik tayininde, AgNP’nin 395.5 nm’deki lokalize yüzey plazmon rezonans (LSPR) absorbsiyon bantı kullanıldı. Deneysel çalışmalarda kullanılan fosfat tampon çözeltisinin optimum pH değerini belirlemek için, farklı pH değerlerindeki siprofloksasin-AgNP’nin absorbans değerindeki değişiklikler incelendi. Dinamik Işın Spektrometresi (DLS) ölçümleri ile AgNP boyutundaki değişiklikler tespit edildi.

Sonuç ve Tartışma: Çalışmamızda siprofloksasinin farmasötik preparatlarda tayini için AgNP’ye dayalı yeni bir spektrofotometrik yöntem geliştirildi. Geliştirilen yöntem için doğrusal çalışma aralığı, pH 6.0 fosfat tamponunda 0.003-3.313 mg/L ve pH 8.0 fosfat tamponunda 0.025-2.50 mg/L olarak belirlendi. Oftalmik solüsyondaki siprofloksasin miktarının belirlenmesi için yapılan geri kazanım çalışmasında geri kazanım değeri %87±3.3 olarak bulundu. Bu veriler ışığında, siprofloksasin tayini için geliştirilen AgNP bazlı yöntemin klinik analizlerde etkili bir analiz yöntemi olarak kullanılabileceği düşünülmektedir.

Anahtar Kelimeler

AgNP, siprofloksasin, spektrofotometri

Destekleyen Kurum

Tübitak

DEVELOPMENT OF NANOPARTICLE BASED SENSITIVE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF CIPROFLOXACIN

Öz

Objective: Ciprofloxacin is a fluoroquinolone antibiotic used in the fight against many bacterial infections. The aim of this study is to develop a rapid and sensitive method for the spectrophotometric determination of metal nanoparticle-based ciprofloxacin.

Material and Method: AgNP was used as metal nanoparticle to develop the method. The localized surface plasmon resonance (LSPR) absorption band of AgNP at 395.5 nm was used for the spectrophotometric determination of ciprofloxacin. In order to determine the optimum pH value of the phosphate buffer solution used in the experimental studies, the changes in the absorbance value of ciprofloxacin-AgNP at different pH values were examined. Changes in AgNP size were detected by DLS measurements.

Result and Discussion: In our study, a new spectrophotometric method based on AgNP was developed for the determination of ciprofloxacin in pharmaceutical preparations. The linear working range for the developed method was determined as 0.003-3.313 mg/L in pH 6.0 phosphate buffer and 0.025-2.50 mg/L in pH 8.0 phosphate buffer. In the recovery study performed to determine the amount of ciprofloxacin in the ophthalmic solution, the recovery value was found to be 87±3.3%. In the light of these data, it is thought that the AgNP-based method developed for the determination of ciprofloxacin can be used as an effective analysis method in clinical analysis.

Anahtar Kelimeler

AgNPs, ciprofloxacine, spectrophotometry

Kaynakça

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