ABAKAVİR, LAMİVUDİN VE ZİDOVUDİN’İN ÜÇLÜ KARIŞIMDA SPEKTROFOTOMETRİK ANALİZİ

Yıl 2023, Cilt: 47 Sayı: 3, 927 - 934, 20.09.2023

Gizem Tırıs , Nevin Erk

https://doi.org/10.33483/jfpau.1329821

Öz

Amaç: Çalışmada abacavir (ABV), lamivudine (LMV) ve zidovudine (ZDV) etken maddelerinin aynı anda tayini ikili amplitüd farkı ile birlikte oran farkı spektrofotometrik yöntemleri uygulanarak yapılmıştır.
Gereç ve Yöntem: İkili amplitüd farkı yönteminde 226.0 nm ve 235.0 nm seçilerek LMV için miktar tayini yapılmıştır. Oran farkı yöntemi için 297.0 nm ve 268.0 nm dalga boyları ve 266.0 nm, 245 nm dalga boyları sırasıyla ABV ve ZDV'nin miktar tayini için seçilmiştir. Yöntemlerin doğruluğu laboratuvar karışımlarına yüzde geri kazanım çalışmaları uygulanarak belirlenmiştir.
Sonuç ve Tartışma: Önerilen çalışmada, HIV tedavisinde kullanılan üç farklı etken maddenin aynı anda miktar tayini yapılmıştır. Yöntemler antiviral ilaçların aynı anda tayini için başarı ile uygulanmıştır. Bu etken maddeler, etkili tedavinin sağlanması için kombinasyon olarak kullanılmaktadır. Uygulanan yöntemler ile, ilk olarak dual amplitut difference yöntemi ile LMV tayin edilmiş sonrasında oran farkı ile ABV ve ZDV tayin edilmiştir. Üç etken madde 3-21 µg/ml konsantrasyon aralığında çalışılmıştır. Korelasyon katsayısı 0.9985 ile 0.9996 olarak bulunmuştur. Geri kazanım sonuçları 95.2 ile 106.2 arasındadır. Yöntemde sadece çözücüde çözünüp ölçülmüştür ön hazırlama işlemi ve pahalı cihazlar olmadan analiz yapılmıştır.

Anahtar Kelimeler

Abacavir, kantitatif belirleme, lamivudin, zidovudin

ANALYSIS OF ABACAVIR LAMIVUDINE AND ZIDOVUDINE BY SPECTROPHOTOMETRIC METHOD IN TRIPLE MIXTURE

Öz

Objective: The simultaneous determination of abacavir (ABV), lamivudine (LMV) and zidovudine (ZDV) were applied by dual amplitude difference method coupled with ratio difference spectrophotometric methods.
Material and Method: The LMV was quantified by selected the 226.0 nm and 235.0 nm in the dual amplitude difference method. For ratio difference method 297.0 nm and 268.0 nm wavelengths and 266.0 nm, 245 nm wavelengths were chosen to quantify respectively ABV and ZDV. Accuracy studies have been carried out with percent recovery.
Result and Discussion: The proposed study, three active substances used in Human immunodeficiency virus (HIV) treatment were quantified. These active ingredients are used in combination to provide effective treatment. With the applied methods, firstly LMV was determined by dual amplitut difference method, then ABV and ZDV were determined by ratio difference. The three active ingredients were studied in the concentration range of 3-21 µg/ml. Correlation coefficients were found to be between 0.9985 and 0.9996. Recovery results range from 95.2 to 106.2. In the method, it was only dissolved in the solvent and measured, and the analysis was carried out without pre-preparation and expensive equipment.

Anahtar Kelimeler

Abacavir, determination, lamivudine, zidovudine

Kaynakça

• 1. Gallant, J.E. (2002). Initial therapy of HIV infection. Journal of Clinical Virology, 25(3), 317-333. [CrossRef]
• 2. Fernández, L.P., Brasca, R., Alcaráz, M.R., Culzoni, M.J. (2018). High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water. Microchemical Journal, 141, 80-86. [CrossRef]
• 3. Yao, L., Dou, W.Y., Ma, Y.F., Liu, Y.S. (2021). Development and validation of sensitive methods for simultaneous determination of 9 antiviral drugs in different various environmental matrices by UPLC-MS/MS. Chemosphere, 282, 131047. [CrossRef]
• 4. Courlet, P., Spaggiari, D., Cavassini, M., Du Pasquier, R., Saldanha, S.A., Buclin, T., Marzolini, C., Csajka, C., Decosterd, L. (2018). Determination of nucleosidic/tidic reverse transcriptase inhibitors in plasma and cerebrospinal fluid by ultra-high-pressure liquid chromatography coupled with tandem mass spectrometry. Clinical Mass Spectrometry, 8, 8-20. [CrossRef]
• 5. Kromdijk, W., Pereira, S.A., Rosing, H., Mulder, J.W., Beijnen, J.H., Huitema, A.D.R. (2013). Development and validation of an assay for the simultaneous determination of zidovudine, abacavir, emtricitabine, lamivudine, tenofovir and ribavirin in human plasma using liquid chromatography-tandem mass spectrometry. Journal of Chromatography B, 919, 43-51. [CrossRef]
• 6. Kumar, D.A., Rao, G.S., Rao, J.V.L.N. (2010). Simultaneous determination of lamivudine, zidovudine and abacavir in tablet dosage forms by RP HPLC method. Journal of Chemistry, 7, 180-184. [CrossRef]
• 7. Rebiere, H., Mazel, B., Civade, C., Bonnet, P.A. (2007). Determination of 19 antiretroviral agents in pharmaceuticals or suspected products with two methods using high-performance liquid chromatography. Journal of Chromatography B, 850(1-2), 376-383. [CrossRef]
• 8. Verweij-van Wissen, C.P.W.G.M., Aarnoutse, R.E., Burger, D.M. (2005). Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography. Journal of Chromatography B, 816(1-2), 121-129. [CrossRef]
• 9. Djurdjevic, P., Laban, A., Markovic, S., Jelikic‐Stankov, M. (2004). Chemometric optimization of a RP‐HPLC method for the simultaneous analysis of abacavir, lamivudine, and zidovudine in tablets. Analytical Letters, 37(13), 2649-2667. [CrossRef]
• 10. Rezk, N.L., Tidwell, R.R., Kashuba, A.D. (2003). Simultaneous determination of six HIV nucleoside analogue reverse transcriptase inhibitors and nevirapine by liquid chromatography with ultraviolet absorbance detection. Journal of Chromatography B, 791(1-2), 137-147. [CrossRef]
• 11. Nadig, S., Jacob, J.T. (2016). A stability indicating RP-HPLC assay method for simultaneous estimation of abacavir, lamivudine, nevirapine and zidovudine in pharmaceutical dosage form. Research Journal of Pharmacy and Technology, 9(11), 1985-1990. [CrossRef]
• 12. Ahmed, D.A., Lotfy, H.M. (2021). Evaluation of in silico and in lab sample enrichment techniques for the assessment of challengeable quaternary combination in critical ratio. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 260, 119943. [CrossRef]
• 13. Płotka-Wasylka, J. (2018). A new tool for the evaluation of the analytical procedure: Green analytical procedure index. Talanta, 181, 204-209. [CrossRef]