DETERMINATION OF DNA DAMAGE INDUCED BY BISPHENOL A AND BISPHENOL S IN MCF7 CELL LINE

Yıl 2024, Cilt: 48 Sayı: 2, 552 - 558, 20.05.2024

Ekin Erdoğmuş , Seda Ipek Tekneci , Belma Gümüşel , Yalçın Duydu , Aylin Üstündağ

https://doi.org/10.33483/jfpau.1428739

Öz

Objective: This study aimed to determine the DNA damage induced by Bisphenol A (BPA) and Bisphenol S (BPS) on MCF7 cell line.
Material and Method: DNA damage was determined by COMET assay in MCF7 cell line at 0.1, 0.5, 1, 5, 10 and 50 μM concentrations of BPA and BPS.
Result and Discussion: All BPA and BPS concentrations studied (0.1, 0.5, 1, 5, 10 and 50 μM) significantly induced DNA damage on MCF7 cell line compared with control (p<0.05). BPS significantly induced DNA damage more than BPA at the 3 highest concentrations studied (5, 10 and 50 μM) (p<0.05). This study shows that bisphenol derivatives can also cause DNA damage like BPA.

Anahtar Kelimeler

Bisphenol A, bisphenol S, COMET assay, DNA damage, MCF7 cell line

Etik Beyan

The authors declare that ethics committee approval is not mandatory for this study.

Destekleyen Kurum

Ankara University Scientific Research Projects Coordination Unit

Proje Numarası

TDK-2022-2702

BİSFENOL A VE BİSFENOL S’NİN MCF7 HÜCRE HATTINDA NEDEN OLDUĞU DNA HASARININ ARAŞTIRILMASI

Öz

Amaç: Bu çalışmada, Bisfenol A (BPA) ve Bisfenol S (BPS)'nin MCF7 hücre hattında neden olduğu DNA hasarının belirlenmesi amaçlanmıştır.
Gereç ve Yöntem: DNA hasarı BPA ve BPS’nin 0.1, 0.5, 1, 5, 10 ve 50 μM konsantrasyonlarda MCF7 hücre hattında COMET yöntemi ile belirlenmiştir.
Sonuç ve Tartışma: Çalışılan tüm BPA ve BPS konsantrasyonları (0.1, 0.5, 1, 5, 10 ve 50 μM) MCF7 hücre hattında kontrole kıyasla önemli ölçüde DNA hasarına neden olmuştur (p<0.05). BPS, çalışılan en yüksek 3 konsantrasyonda (5, 10 ve 50 μM) DNA hasarını BPA'dan daha fazla indüklemiştir (p<0.05). Bu çalışma bisfenol türevlerinin de BPA gibi DNA hasarına neden olabileceğini göstermektedir.

Anahtar Kelimeler

Bisfenol A, bisfenol S, COMET testi, DNA hasarı, MCF7 hücre hattı

Proje Numarası

TDK-2022-2702

Kaynakça

• 1. Vogel, S.A. (2009). The politics of plastics: the making and unmaking of bisphenol a “safety”. American Journal of Public Health, 99(S3), S559-S566. [CrossRef]
• 2. Abraham, A., Chakraborty, P. (2020). A review on sources and health impacts of bisphenol A. Reviews On Environmental Health, 35(2), 201-210. [CrossRef]
• 3. Michałowicz, J. (2014). Bisphenol A-sources, toxicity and biotransformation. Environmental Toxicology And Pharmacology, 37(2), 738-758. [CrossRef]
• 4. Cwiek-Ludwicka, K. (2015). Bisphenol A (BPA) in food contact materials-new scientific opinion from EFSA regarding public health risk. Roczniki Państwowego Zakładu Higieny, 66(4).
• 5. Bonefeld-Jørgensen, E.C., Long, M., Hofmeister, M.V., Vinggaard, A.M. (2007). Endocrine-disrupting potential of bisphenol A, bisphenol A dimethacrylate, 4-n-nonylphenol, and 4-n-octylphenol in vitro: New data and a brief review. Environmental Health Perspectives, 115(Suppl 1), 69-76. [CrossRef]
• 6. Richter, C.A., Birnbaum, L.S., Farabollini, F., Newbold, R.R., Rubin, B.S., Talsness, C.E., Vom Saal, F.S. (2007). In vivo effects of bisphenol A in laboratory rodent studies. Reproductive Toxicology, 24(2), 199-224. [CrossRef]
• 7. Rochester, J.R. (2013). Bisphenol A and human health: a review of the literature. Reproductive Toxicology, 42, 132-155. [CrossRef]
• 8. Ayazgök, B., Küçükkilinç, T.T. (2017). Düşük doz bisfenol A'nın büyük etkileri. FABAD Journal of Pharmaceutical Sciences, 42(2), 139.
• 9. American Type Culture Collection (ATCC) Web site. https://www.atcc.org/products/htb-22. Accessed date: 23.02.2024.
• 10. Perera, L., Li, Y., Coons, L.A., Houtman, R., van Beuningen, R., Goodwin, B., Aurerbach, S.S., Teng, C.T. (2017). Binding of bisphenol A, bisphenol AF, and bisphenol S on the androgen receptor: Coregulator recruitment and stimulation of potential interaction sites. Toxicology in Vitro, 44, 287-302. [CrossRef]
• 11. Presunto, M., Mariana, M., Lorigo, M., Cairrao, E. (2023). The effects of bisphenol A on human male infertility: A review of current epidemiological studies. International Journal of Molecular Sciences, 24(15), 12417. [CrossRef]
• 12. Goodman, J.E., Peterson, M.K., Hixon, M.L., Shubin, S.P. (2017). Derivation of an oral maximum allowable dose level for Bisphenol A. Regulatory Toxicology and Pharmacology, 86, 312-318. [CrossRef]
• 13. İyigündoğdu, İ., Üstündağ, A., Duydu, Y. (2020). Toxicological evaluation of bisphenol A and its analogues. Turkish Journal of Pharmaceutical Sciences, 17(4), 457. [CrossRef]
• 14. Mustieles, V., d'Cruz, S.C., Couderq, S., Rodríguez-Carrillo, A., Fini, J.B., Hofer, T., Steffensen, I.L., Dirven, H., Barouki, R., Olea, N., Fernández, M.F., David, A. (2020). Bisphenol A and its analogues: A comprehensive review to identify and prioritize effect biomarkers for human biomonitoring. Environment International, 144, 105811. [CrossRef]
• 15. Thoene, M., Dzika, E., Gonkowski, S., Wojtkiewicz, J. (2020). Bisphenol S in food causes hormonal and obesogenic effects comparable to or worse than bisphenol A: A literature review. Nutrients, 12(2), 532. [CrossRef]
• 16. Catenza, C.J., Farooq, A., Shubear, N.S., Donkor, K.K. (2021). A targeted review on fate, occurrence, risk and health implications of bisphenol analogues. Chemosphere, 268, 129273. [CrossRef]
• 17. İpek, S., İyigündoğdu, İ., Üstündağ, A., Duydu, Y. (2022). Evaluation of the cytotoxic effect of bisphenol A and its analogs in MCF-7 and HSeC cell lines in vitro. Fabad Journal of Pharmaceutical Sciences, 1(47), 13-22. [CrossRef]
• 18. Singh, N.P., McCoy, M.T., Tice, R.R., Schneider, E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1), 184-191. [CrossRef]
• 19. He, Q.Z., Zhu, B.Q., Xu, X.N., Zeng, H.C. (2021). Role of the BDNF/TrkB/CREB signaling pathway in the cytotoxicity of bisphenol S in SK‐N‐SH cells. Journal of Biochemical and Molecular Toxicology, 35(6), 1-11. [CrossRef]
• 20. Feng, Y., Jiao, Z., Shi, J., Li, M., Guo, Q., Shao, B. (2016). Effects of bisphenol analogues on steroidogenic gene expression and hormone synthesis in H295R cells. Chemosphere, 147, 9-19. [CrossRef]
• 21. George, V.C., Rupasinghe, H.V. (2018). DNA damaging and apoptotic potentials of bisphenol A and bisphenol S in human bronchial epithelial cells. Environmental Toxicology and Pharmacology, 60, 52-57. [CrossRef]
• 22. Mokra, K., Kuźmińska-Surowaniec, A., Woźniak, K., Michałowicz, J. (2017). Evaluation of DNA-damaging potential of bisphenol A and its selected analogs in human peripheral blood mononuclear cells (in vitro study). Food and Chemical Toxicology, 100, 62-69. [CrossRef]
• 23. Fic, A., Sollner Dolenc, M., Filipič, M., Peterlin Mašić, L. (2013). Mutagenicity and DNA damage of bisphenol A and its structural analogues in HepG2 cells. Arhiv Za Higijenu Rada I Toksikologiju, 64(2), 189-199. [CrossRef]
• 24. Kose, O., Rachidi, W., Beal, D., Erkekoglu, P., Fayyad‐Kazan, H., Kocer Gumusel, B. (2020). The effects of different bisphenol derivatives on oxidative stress, DNA damage and DNA repair in RWPE‐1 cells: A comparative study. Journal of Applied Toxicology, 40(5), 643-654. [CrossRef]
• 25. Huang, W., Zhao, C., Zhong, H., Zhang, S., Xia, Y., Cai, Z. (2019). Bisphenol S induced epigenetic and transcriptional changes in human breast cancer cell line MCF-7. Environmental Pollution, 246, 697-703. [CrossRef]