TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS

Yıl 2024, Cilt: 48 Sayı: 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), 3 - 3

Laid Ziane , Abdelaziz Berreghioua

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Objective: This study sought to identify potential sources for upcoming novel antioxidants in food and pharmaceutical formulations by screening various solvent extracts from the leaves of Atriplex halimus Lin. for their ability to exhibit strong antioxidant activity in vitro, as well as their total phenolic and flavonoid contents.
Material and Method: To determine the total amount of polyphenols and flavonoids in Atriplex halimus extracts, including ethyl ether, ethyl acetate, and n-butanol extracts, as well as their corresponding impact on this plant's antioxidant activity, were carried out using the conventional procedures.
Result and Discussion: In the current investigation, total phenolic and flavonoid contents in butanolic extract were found to be 68.20 mg gallic acid equivalent (GAE)/g dry extract) and 439 mg quercetin equivalent (QE)/g dry extract. The hydro-alcoholic extract was extracted by liquid/liquid partition with solvents of increasing polarity: ethyl ether, ethyl acetate and n-butanol) by the free radical DPPH removing garbage and HPTLC as well as their reduction kinetics. It was found that the extract of butanol and ethyl acetate had powerful uplifting power garbage DPPH with IC50 values of 2.1959 and 2.4234 mg/ml, respectively.

Anahtar Kelimeler

Antioxidant activity, Atriplex halimus, bioactive extract, DPPH, phytochemical, quercetin

ATRIPLEX HALIMUS YAPRAKLARINDAN ELDE EDİLEN BİYOAKTİF EKSTRAKTLARIN TOPLAM FENOLIK VE FLAVONOID ÖLÇÜMÜ VE ANTİOKSIDAN AKTİVİTESİ

Öz

Amaç: Bu çalışmada, Atriplex halimus bitkisinin tamamının çeşitli solvent ekstrelerini, in vitro güçlü antioksidan aktivite sergileme yetenekleri ve ayrıca toplam fenolik ve flavonoid içerikleri açısından tarayarak, gıda ve farmasötik formülasyonlarda gelecek yeni antioksidanlar için potansiyel kaynakları belirleme amaçlandı.
Gereç ve Yöntem: Etil eter, etil asetat ve n-butanol ekstreleri dahil olmak üzere Atripleks halimus ekstrelerindeki polifenollerin ve flavonoidlerin toplam miktarının yanı sıra bunların bu bitkinin antioksidan aktivitesi üzerindeki karşılık gelen etkilerini belirlemek için geleneksel prosedürler kullanılarak gerçekleştirildi.
Sonuç ve Tartışma: Mevcut araştırmada, bütanolik ekstredeki toplam fenolik ve flavonoid içeriğinin 68.20 mg gallik asit eşdeğeri (GAE)/g kuru ekstrakt) ve 439 mg kersetin eşdeğeri (QE)/g kuru ekstre olduğu bulunmuştur. Hidro-alkolik ekstresi, artan polariteye sahip solventler (etil eter, etil asetat ve n-butanol) ile sıvı/sıvı partitisyona tabi tutulmasıyla, serbest radikal DPPH'nin atıkları ve HPTLC'yi ve bunların indirgeme kinetiklerini ortadan kaldırmasıyla ekstre edildi. Butanol ve etil asetat ekstreleri sırasıyla 2.1959 ve 2.4234 mg/ml IC50 değerleriyle DPPH radikali süpürücü aktivitesine sahip olduğu bulundu.

Anahtar Kelimeler

Antioksidan aktivite, Atriplex halimus, biyoaktif ekstre, DPPH, fitokimyasal, quercetin

Kaynakça

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