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TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS

Yıl 2024, Cilt: 48 Sayı: 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), 1313 - 1317, 25.11.2024

Öz

Objective: This study sought to identify potential sources for upcoming novel antioxidants in food and pharmaceutical formulations by screening various solvent extracts from the leaves of Atriplex halimus Lin. for their ability to exhibit strong antioxidant activity in vitro, as well as their total phenolic and flavonoid contents.
Material and Method: To determine the total amount of polyphenols and flavonoids in Atriplex halimus extracts, including ethyl ether, ethyl acetate, and n-butanol extracts, as well as their corresponding impact on this plant's antioxidant activity, were carried out using the conventional procedures.
Result and Discussion: In the current investigation, total phenolic and flavonoid contents in butanolic extract were found to be 68.20 mg gallic acid equivalent (GAE)/g dry extract) and 439 mg quercetin equivalent (QE)/g dry extract. The hydro-alcoholic extract was extracted by liquid/liquid partition with solvents of increasing polarity: ethyl ether, ethyl acetate and n-butanol) by the free radical DPPH removing garbage and HPTLC as well as their reduction kinetics. It was found that the extract of butanol and ethyl acetate had powerful uplifting power garbage DPPH with IC50 values of 2.1959 and 2.4234 mg/ml, respectively.

Kaynakça

  • 1. Slimani, A., Moussaoui, A., Laazouni, A., Hamadi, N. (2016). Phytochemical screening, contribution to the study of the antifungal effect of flavonoids from different parts of Ziziphus lotus of South-West Algeria. Asian Journal of Pharmaceutical and Clinical Research, 10(2), 20.
  • 2. Lim, Y., Lim, T., Tee, J. (2007). Antioxidant properties of several tropical fruits: A comparative study. Food Chemistry, 103, 1003-1008. [CrossRef]
  • 3. Ismail, A., Marjan, Z., Foong, C. (2004). Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry, 87, 581-586. [CrossRef]
  • 4. Benhammou, N., Bekkara, F.A., Panovska, T.K. (2009). Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus. Comptes Rendus Chimie, 12, 1259-1266. [CrossRef]
  • 5. Abd El-Rahman, H.H., Mohamed, M.I., Gehad, A.E.A., Awadallah, I.M. (2006). Ameliorating the anti-nutritional factors effect in Atriplex halimus on sheep and goats by ensiling or polyethylene glycol (PEG) supplementation. International Journal of Agriculture and Biology, 8, 766.
  • 6. Ziane, L., Lazouni, H.A., Moussaoui, A., Namidi, N., Djellouli, M., Belabbes, A. (2015). Flavonoid from methanolic extract of Limoniastrum feei (girard) batt (Plumbaginaceae). Asian Journal of Pharmaceutical and Clinical Research, 8, 218-219.
  • 7. King, M.B., Bott, T.R. (1993). Extraction of Natural Products Using Near- Critical Solvents. Chapman & Hall, Glasgow, p.325.
  • 8. Kumar, G.P., Kumar, S.V., Kumar, A.S. (2017). Quantification of total phenolic and flavonoid contents, and evaluation of free radical scavenging potential of Vernonia cinerea. Asian Pacific Journal of Health Sciences. 4(3), 279-287. [CrossRef]
  • 9. Rajappa, K.C., Sasidhar, B.S., Naika, M., Keri, R.S. (2018). Phytochemical composition of Caesalpinia crista extract as potential source for inhibiting cholinesterase and β-amyloid aggregation: Significance to Alzheimer's disease. Asian Pacific Journal of Tropical Biomedicine. 8(10), 500-512. [CrossRef]
  • 10. Rydberg, J. (2004). Solvent Extraction Principles and Practice. CRC Press, Boca Raton, p.480.
  • 11. Rosset, R., Jardy A., Caude, M. (1991). Chromatographie en phase liquide et supercitique, Masson, Paris, p.919.
  • 12. Hostettman, K., Marston, A., Hostettman, M. (1998). Preparative chromatography techniques: Applications in natural product isolation, Springer: Berlin Heidelberg. p.240.
  • 13. Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200. [CrossRef]
  • 14. Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for the antioxidant activity. Songklanakarin Journal of Sciences and Technology, 26, 211-219.
  • 15. Raquibul Hasan, S.M., Mokarram Hossain, M., Raushanara, A., Mariam, J., Ehsanul Hoque, M., Shafiqur, R. (2009). DPPH free radical scavenging activity of some Bangladeshi medicinal plants. Journal of Medicinal Plants Research, 3(11), 875-879.
  • 16. Mahmoudi, S., Khali, M., Benkhaled, A., Benamirouche, K., Baiti, I. (2016). Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaves extracts from ten Algerian Ficus carica L. varieties. Asian Pacific Journal of Tropical Biomedicine, 6, 239-245. [CrossRef]
  • 17. Ziane, L., Djellouli, M., Berghioua, A. (2021). Short Communication: Chemical composition, antioxidant andantimicrobial activity of Fagonia longispina (Zygophyllaceae) of Algeria. Biodiversitas Journal of Biological Diversity, 22, 3448-3453. [CrossRef]

ATRIPLEX HALIMUS YAPRAKLARINDAN ELDE EDİLEN BİYOAKTİF EKSTRAKTLARIN TOPLAM FENOLİK VE FLAVONOİD ÖLÇÜMÜ VE ANTİOKSİDAN AKTİVİTESİ

Yıl 2024, Cilt: 48 Sayı: 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), 1313 - 1317, 25.11.2024

Öz

Amaç: Bu çalışmada, Atriplex halimus bitkisinin tamamının çeşitli solvent ekstrelerini, in vitro güçlü antioksidan aktivite sergileme yetenekleri ve ayrıca toplam fenolik ve flavonoid içerikleri açısından tarayarak, gıda ve farmasötik formülasyonlarda gelecek yeni antioksidanlar için potansiyel kaynakları belirleme amaçlandı.
Gereç ve Yöntem: Etil eter, etil asetat ve n-butanol ekstreleri dahil olmak üzere Atripleks halimus ekstrelerindeki polifenollerin ve flavonoidlerin toplam miktarının yanı sıra bunların bu bitkinin antioksidan aktivitesi üzerindeki karşılık gelen etkilerini belirlemek için geleneksel prosedürler kullanılarak gerçekleştirildi.
Sonuç ve Tartışma: Mevcut araştırmada, bütanolik ekstredeki toplam fenolik ve flavonoid içeriğinin 68.20 mg gallik asit eşdeğeri (GAE)/g kuru ekstrakt) ve 439 mg kersetin eşdeğeri (QE)/g kuru ekstre olduğu bulunmuştur. Hidro-alkolik ekstresi, artan polariteye sahip solventler (etil eter, etil asetat ve n-butanol) ile sıvı/sıvı partitisyona tabi tutulmasıyla, serbest radikal DPPH'nin atıkları ve HPTLC'yi ve bunların indirgeme kinetiklerini ortadan kaldırmasıyla ekstre edildi. Butanol ve etil asetat ekstreleri sırasıyla 2.1959 ve 2.4234 mg/ml IC50 değerleriyle DPPH radikali süpürücü aktivitesine sahip olduğu bulundu.

Kaynakça

  • 1. Slimani, A., Moussaoui, A., Laazouni, A., Hamadi, N. (2016). Phytochemical screening, contribution to the study of the antifungal effect of flavonoids from different parts of Ziziphus lotus of South-West Algeria. Asian Journal of Pharmaceutical and Clinical Research, 10(2), 20.
  • 2. Lim, Y., Lim, T., Tee, J. (2007). Antioxidant properties of several tropical fruits: A comparative study. Food Chemistry, 103, 1003-1008. [CrossRef]
  • 3. Ismail, A., Marjan, Z., Foong, C. (2004). Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry, 87, 581-586. [CrossRef]
  • 4. Benhammou, N., Bekkara, F.A., Panovska, T.K. (2009). Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus. Comptes Rendus Chimie, 12, 1259-1266. [CrossRef]
  • 5. Abd El-Rahman, H.H., Mohamed, M.I., Gehad, A.E.A., Awadallah, I.M. (2006). Ameliorating the anti-nutritional factors effect in Atriplex halimus on sheep and goats by ensiling or polyethylene glycol (PEG) supplementation. International Journal of Agriculture and Biology, 8, 766.
  • 6. Ziane, L., Lazouni, H.A., Moussaoui, A., Namidi, N., Djellouli, M., Belabbes, A. (2015). Flavonoid from methanolic extract of Limoniastrum feei (girard) batt (Plumbaginaceae). Asian Journal of Pharmaceutical and Clinical Research, 8, 218-219.
  • 7. King, M.B., Bott, T.R. (1993). Extraction of Natural Products Using Near- Critical Solvents. Chapman & Hall, Glasgow, p.325.
  • 8. Kumar, G.P., Kumar, S.V., Kumar, A.S. (2017). Quantification of total phenolic and flavonoid contents, and evaluation of free radical scavenging potential of Vernonia cinerea. Asian Pacific Journal of Health Sciences. 4(3), 279-287. [CrossRef]
  • 9. Rajappa, K.C., Sasidhar, B.S., Naika, M., Keri, R.S. (2018). Phytochemical composition of Caesalpinia crista extract as potential source for inhibiting cholinesterase and β-amyloid aggregation: Significance to Alzheimer's disease. Asian Pacific Journal of Tropical Biomedicine. 8(10), 500-512. [CrossRef]
  • 10. Rydberg, J. (2004). Solvent Extraction Principles and Practice. CRC Press, Boca Raton, p.480.
  • 11. Rosset, R., Jardy A., Caude, M. (1991). Chromatographie en phase liquide et supercitique, Masson, Paris, p.919.
  • 12. Hostettman, K., Marston, A., Hostettman, M. (1998). Preparative chromatography techniques: Applications in natural product isolation, Springer: Berlin Heidelberg. p.240.
  • 13. Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200. [CrossRef]
  • 14. Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for the antioxidant activity. Songklanakarin Journal of Sciences and Technology, 26, 211-219.
  • 15. Raquibul Hasan, S.M., Mokarram Hossain, M., Raushanara, A., Mariam, J., Ehsanul Hoque, M., Shafiqur, R. (2009). DPPH free radical scavenging activity of some Bangladeshi medicinal plants. Journal of Medicinal Plants Research, 3(11), 875-879.
  • 16. Mahmoudi, S., Khali, M., Benkhaled, A., Benamirouche, K., Baiti, I. (2016). Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaves extracts from ten Algerian Ficus carica L. varieties. Asian Pacific Journal of Tropical Biomedicine, 6, 239-245. [CrossRef]
  • 17. Ziane, L., Djellouli, M., Berghioua, A. (2021). Short Communication: Chemical composition, antioxidant andantimicrobial activity of Fagonia longispina (Zygophyllaceae) of Algeria. Biodiversitas Journal of Biological Diversity, 22, 3448-3453. [CrossRef]
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılıkta Analitik Kimya
Bölüm Araştırma Makalesi
Yazarlar

Laid Ziane 0000-0002-4243-0557

Abdelaziz Berreghioua 0000-0002-8100-8025

Erken Görünüm Tarihi 13 Kasım 2024
Yayımlanma Tarihi 25 Kasım 2024
Gönderilme Tarihi 29 Mart 2024
Kabul Tarihi 23 Temmuz 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 48 Sayı: 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023)

Kaynak Göster

APA Ziane, L., & Berreghioua, A. (2024). TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS. Journal of Faculty of Pharmacy of Ankara University, 48(4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), 1313-1317. https://doi.org/10.33483/jfpau.1461426
AMA Ziane L, Berreghioua A. TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS. Ankara Ecz. Fak. Derg. Kasım 2024;48(4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023):1313-1317. doi:10.33483/jfpau.1461426
Chicago Ziane, Laid, ve Abdelaziz Berreghioua. “TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS”. Journal of Faculty of Pharmacy of Ankara University 48, sy. 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023) (Kasım 2024): 1313-17. https://doi.org/10.33483/jfpau.1461426.
EndNote Ziane L, Berreghioua A (01 Kasım 2024) TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS. Journal of Faculty of Pharmacy of Ankara University 48 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023) 1313–1317.
IEEE L. Ziane ve A. Berreghioua, “TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS”, Ankara Ecz. Fak. Derg., c. 48, sy. 4-1, ss. 1313–1317, 2024, doi: 10.33483/jfpau.1461426.
ISNAD Ziane, Laid - Berreghioua, Abdelaziz. “TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS”. Journal of Faculty of Pharmacy of Ankara University 48/4-1 (Kasım 2024), 1313-1317. https://doi.org/10.33483/jfpau.1461426.
JAMA Ziane L, Berreghioua A. TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS. Ankara Ecz. Fak. Derg. 2024;48:1313–1317.
MLA Ziane, Laid ve Abdelaziz Berreghioua. “TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS”. Journal of Faculty of Pharmacy of Ankara University, c. 48, sy. 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), 2024, ss. 1313-7, doi:10.33483/jfpau.1461426.
Vancouver Ziane L, Berreghioua A. TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS. Ankara Ecz. Fak. Derg. 2024;48(4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023):1313-7.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.