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THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE

Yıl 2024, Cilt: 48 Sayı: 3, 1098 - 1109, 10.09.2024
https://doi.org/10.33483/jfpau.1505078

Öz

Objective: Dobutamine (DBT), one of the most common synthetic catecholamines, is important in the renal, cardiovascular, hormonal, and central nervous systems. In our study, it is proposed to produce the first MIP-based electrochemical sensor for DBT analysis. MIP(DBT)/GCE was developed by electropolymerization of 4-aminobenzoic acid (4-ABA) in the presence of DBT. Analytical performance and validation evaluation were performed on both standard solution and commercial serum samples.
Material and Method: DBT was supplied by the Vem pharmaceutical company. For the preparation of 5 mM [Fe(CN)6]3-/4- solution, known amounts of potassium ferricyanide ([K3Fe(CN)6]) and potassium ferrocyanide (K4[Fe(CN)6].3H2O mixed in 0.1 M KCl solution. The 4-ABA was used to create the polymeric film in the presence of DBT. Electrochemical measurements were actualized in IVIUM compactStat.h potentiostat (Eindhoven, The Netherlands) using a three-electrode system consisting of Pt wire, Ag/AgCl reference electrode, and glassy carbon electrode (GCE).
Result and Discussion: The MIP(DBT)/GCE sensor was applied to standard solution and commercial serum samples. When DBT concentrations were plotted against ΔI values, a linear response between 1×10-13 and 1×10-12 M was obtained in both environments. LOD results were found to be 0.087×10-13 M and 0.033×10-13 M in standard solution and commercial human serum, respectively. Recovery% and RSD% were found to be 99.68-100.32% and 1.38-1.66%, respectively.

Kaynakça

  • 1. Takkis, K., Veigure, R., Metsvaht, T., Hallik, M., Ilmoja, M.L., Starkopf, J., Kipper, K. (2019). A sensitive method for the simultaneous UHPLC-MS/MS analysis of milrinone and dobutamine in blood plasma using NH4F as the eluent additive and ascorbic acid as a stabilizer. Clinical Mass Spectrometry, 12, 23-29. [CrossRef]
  • 2. Long, Z., Zhan, Z., Guo, Z., Li, Y., Yao, J., Ji, F., Li, C., Zheng, X., Ren, B., Huang, T. (2019). A novel two-dimensional liquid chromatography - Mass spectrometry method for direct drug impurity identification from HPLC eluent containing ion-pairing reagent in mobile phases. Analytical Chemistry Acta, 1049, 105-114. [CrossRef]
  • 3. Albóniga, O.E., Alonso, M.L., Blanco, M.E., González, O., Grisaleña, A., Campanero, M.A., Alonso, R.M. (2017). Quantitative determination of dobutamine in newborn pig plasma samples by HPLC-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 145, 178-185. [CrossRef]
  • 4. Thippani, R., Pothuraju, N.R., Ramisetti, N.R., Shaik, S. (2013). Optimization and validation of a fast RP-HPLC method for the determination of dobutamine in rat plasma: Pharmacokinetic studies in healthy rat subjects. Journal of Pharmaceutical Analysis, 3(6), 434-439. [CrossRef]
  • 5. Meineke, I., Steinmetz, H., Kramer, S., Gleiter, C.H. (2002). Determination of fenoterol in human plasma by HPLC with fluorescence detection after derivatization. Journal of Pharmaceutical and Biomedical Analysis, 29, (1-2), 147-152. [CrossRef]
  • 6. Kramer, S., Blaschkë¨institute, G. (2001). High-performance liquid chromatographic determination of the b-selective adrenergic agonist fenoterol in human plasma after 2 fluorescence derivatization. Journal of Chromatography B: Biomedical Sciences and Applications, 751(1), 169-175. [CrossRef]
  • 7. Ozkan, S.A., Kauffmann, J.M., Zuman, P. (2015). Electroanalytical method validation method validation in pharmaceutical analysis and their applications, Springer, Berlin, pp.235-266. [CrossRef]
  • 8. Saputra, H.A. (2023). Electrochemical sensors: Basic principles, engineering, and state of the art. Monatshefte Für Chemie - Chemical Monthly, 154(10), 1083-1100. [CrossRef]
  • 9. Omar, F.S., Duraisamy, N., Ramesh, K., Ramesh, S. (2016). Conducting polymer and its composite materials based electrochemical sensor for Nicotinamide Adenine Dinucleotide (NADH). Biosensors and Bioelectronics, 79, 763-775. [CrossRef]
  • 10. Shvedene, N.V, Nazarova, I.A., Formanovsky, A.A., Otkidach, D.S., Pletnev, I.V. (2002). 3-(4- Tolylazo)phenylboronic acid as the active component of polyhydroxy compounds-selective electrodes. Electrochemistry Communications, 4 (12), 978-984. [CrossRef]
  • 11. Zhang, Y. (2004). Voltammetric behavior of dobutamine at poly(acridine orange) film modified electrode and its determination by adsorptive stripping voltammetry. Analytical Letters, 37(10), 2031-2042. [CrossRef]
  • 12. Chernyshov, D.V., Shvedene, N.V., Antipova, E.R., Pletnev, I.V. (2008). Ionic liquid-based miniature electrochemical sensors for the voltammetric determination of catecholamines. Analytical Chimica Acta, 621(2), 178-184. [CrossRef]
  • 13. Rastogi, P.K., Ganesan, V., Krishnamoorthi, S. (2012). Ion exchange voltammetry at permselective copolymer modified electrode and its application for the determination of catecholamines. Journal of Electroanalytical Chemistry, 676, 13-19. [CrossRef]
  • 14. Ling, Y.Y., Huang, Q.A., Feng, D.X., Li, X.Z., Wei, Y. (2013). Electrochemical oxidation of dobutamine on a magnesium oxide microflowers-nafion composite film modified glassy carbon electrode. Analytical Methods, 5(18), 4580-4584. [CrossRef]
  • 15. Asadian, E., Shahrokhian, S., Zad, A.I., Jokar, E. (2014). In-situ electro-polymerization of graphene nanoribbon/polyaniline composite film: Application to sensitive electrochemical detection of dobutamine. Sensors and Actuators B: Chemical, 196, 582-588. [CrossRef]
  • 16. El-Ads, E.H., Atta, N.F., Galal, A., El-Gohary, A.R.M. (2018). Nano-perovskite decorated carbon nanotubes composite for ultrasensitive determination of a cardio-stimulator drug. Journal of Electroanalytical Chemistry, 816, 149-159. [CrossRef]
  • 17. Atta, N.F., Galal, A., Ahmed, Y.M., El-Ads, E.H. (2019). Design strategy and preparation of a conductive layered electrochemical sensor for simultaneous determination of ascorbic acid, dobutamine, acetaminophen and amlodipine. Sensors and Actuators B: Chemical, 297, 126648. [CrossRef]
  • 18. Ibrahim, H., Temerk, Y. (2020). Synergistic electrocatalytic activity of In2O3@FMWCNTs nanocomposite for electrochemical quantification of dobutamine in clinical patient blood and in injection dosage form. Talanta, 208, 120362. [CrossRef]
  • 19. Chowdhury, R., Maranas, C.D. (2020). From directed evolution to computational enzyme engineering-A review. AIChE Journal, 66(3), e16847. [CrossRef]
  • 20. Chen, L., Wang, X., Lu, W., Wu, X., Li, J. (2016). Molecular imprinting: Perspectives and applications. Chemical Society Reviews, 45(8), 2137-2211. [CrossRef]
  • 21. Uzun, L., Turner, A.P.F. (2016). Molecularly-imprinted polymer sensors: Realising their potential. Biosens Bioelectron, 76, 131-144. [CrossRef]
  • 22. European Medicines Agency. (1995). Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonisation (ICH) Guideline ICH Topic.

DOBUTAMİN TAYİNİ İÇİN MIP ESASLI ELEKTROKİMYASAL SENSÖRÜN GELİŞTİRİLMESİ

Yıl 2024, Cilt: 48 Sayı: 3, 1098 - 1109, 10.09.2024
https://doi.org/10.33483/jfpau.1505078

Öz

Amaç: En yaygın sentetik katekolaminlerden biri olan dobutamin (DBT), renal, kardiyovasküler, hormonal ve merkezi sinir sistemlerinde önemlidir. Çalışmamızda DBT analizi için ilk MIP tabanlı elektrokimyasal sensörün üretilmesi önerilmiştir. MIP(DBT)/GCE, 4-aminobenzoik asidin (4-ABA) DBT varlığında elektropolimerleştirilmesiyle geliştirildi. Analitik performans ve doğrulama değerlendirmesi hem standart çözelti hem de ticari serum örneklerinde gerçekleştirildi.
Gereç ve Yöntem: DBT, Vem ilaç firmasından temin edilmiştir. 5 mM [Fe(CN)6]3-/4- çözeltisinin hazırlanması için, potasyum ferrisiyanür ([K3Fe(CN)6]), potasyum ferrosiyanür (K4[Fe(CN)) 6].3H2O, 0.1 M KCl'de karıştırıldı. DBT varlığında polimerik filmi oluşturmak için 4-ABA kullanıldı. Elektrokimyasal ölçümler, IVIUM kompaktStat.h potansiyostatta (Eindhoven, Hollanda) Pt teli, Ag/AgCl referans elektrotu ve camsı karbon elektrottan (GCE) oluşan üç elektrotlu bir sistem kullanılarak gerçekleştirildi.
Sonuç ve Tartışma: MIP(DBT)/GCE sensörü, DBT'yi belirlemek için standart çözeltide ve insan serumunun ticari numunelerinde test edildi. DBT konsantrasyonları ΔI değerlerine göre çizildiğinde her iki ortamda da 1×10-13 ile 1×10-12 M arasında doğrusal bir yanıt elde edildi. TS sonuçları ise sırasıyla standart çözelti ve ticari insan serumda 0.087×10-13 ve 0.033×10-13 olarak bulundu. %Geri kazanım ve %BSS sırasıyla %99.68-100.32 ve %1.38-1.66 olarak belirlendi.

Kaynakça

  • 1. Takkis, K., Veigure, R., Metsvaht, T., Hallik, M., Ilmoja, M.L., Starkopf, J., Kipper, K. (2019). A sensitive method for the simultaneous UHPLC-MS/MS analysis of milrinone and dobutamine in blood plasma using NH4F as the eluent additive and ascorbic acid as a stabilizer. Clinical Mass Spectrometry, 12, 23-29. [CrossRef]
  • 2. Long, Z., Zhan, Z., Guo, Z., Li, Y., Yao, J., Ji, F., Li, C., Zheng, X., Ren, B., Huang, T. (2019). A novel two-dimensional liquid chromatography - Mass spectrometry method for direct drug impurity identification from HPLC eluent containing ion-pairing reagent in mobile phases. Analytical Chemistry Acta, 1049, 105-114. [CrossRef]
  • 3. Albóniga, O.E., Alonso, M.L., Blanco, M.E., González, O., Grisaleña, A., Campanero, M.A., Alonso, R.M. (2017). Quantitative determination of dobutamine in newborn pig plasma samples by HPLC-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 145, 178-185. [CrossRef]
  • 4. Thippani, R., Pothuraju, N.R., Ramisetti, N.R., Shaik, S. (2013). Optimization and validation of a fast RP-HPLC method for the determination of dobutamine in rat plasma: Pharmacokinetic studies in healthy rat subjects. Journal of Pharmaceutical Analysis, 3(6), 434-439. [CrossRef]
  • 5. Meineke, I., Steinmetz, H., Kramer, S., Gleiter, C.H. (2002). Determination of fenoterol in human plasma by HPLC with fluorescence detection after derivatization. Journal of Pharmaceutical and Biomedical Analysis, 29, (1-2), 147-152. [CrossRef]
  • 6. Kramer, S., Blaschkë¨institute, G. (2001). High-performance liquid chromatographic determination of the b-selective adrenergic agonist fenoterol in human plasma after 2 fluorescence derivatization. Journal of Chromatography B: Biomedical Sciences and Applications, 751(1), 169-175. [CrossRef]
  • 7. Ozkan, S.A., Kauffmann, J.M., Zuman, P. (2015). Electroanalytical method validation method validation in pharmaceutical analysis and their applications, Springer, Berlin, pp.235-266. [CrossRef]
  • 8. Saputra, H.A. (2023). Electrochemical sensors: Basic principles, engineering, and state of the art. Monatshefte Für Chemie - Chemical Monthly, 154(10), 1083-1100. [CrossRef]
  • 9. Omar, F.S., Duraisamy, N., Ramesh, K., Ramesh, S. (2016). Conducting polymer and its composite materials based electrochemical sensor for Nicotinamide Adenine Dinucleotide (NADH). Biosensors and Bioelectronics, 79, 763-775. [CrossRef]
  • 10. Shvedene, N.V, Nazarova, I.A., Formanovsky, A.A., Otkidach, D.S., Pletnev, I.V. (2002). 3-(4- Tolylazo)phenylboronic acid as the active component of polyhydroxy compounds-selective electrodes. Electrochemistry Communications, 4 (12), 978-984. [CrossRef]
  • 11. Zhang, Y. (2004). Voltammetric behavior of dobutamine at poly(acridine orange) film modified electrode and its determination by adsorptive stripping voltammetry. Analytical Letters, 37(10), 2031-2042. [CrossRef]
  • 12. Chernyshov, D.V., Shvedene, N.V., Antipova, E.R., Pletnev, I.V. (2008). Ionic liquid-based miniature electrochemical sensors for the voltammetric determination of catecholamines. Analytical Chimica Acta, 621(2), 178-184. [CrossRef]
  • 13. Rastogi, P.K., Ganesan, V., Krishnamoorthi, S. (2012). Ion exchange voltammetry at permselective copolymer modified electrode and its application for the determination of catecholamines. Journal of Electroanalytical Chemistry, 676, 13-19. [CrossRef]
  • 14. Ling, Y.Y., Huang, Q.A., Feng, D.X., Li, X.Z., Wei, Y. (2013). Electrochemical oxidation of dobutamine on a magnesium oxide microflowers-nafion composite film modified glassy carbon electrode. Analytical Methods, 5(18), 4580-4584. [CrossRef]
  • 15. Asadian, E., Shahrokhian, S., Zad, A.I., Jokar, E. (2014). In-situ electro-polymerization of graphene nanoribbon/polyaniline composite film: Application to sensitive electrochemical detection of dobutamine. Sensors and Actuators B: Chemical, 196, 582-588. [CrossRef]
  • 16. El-Ads, E.H., Atta, N.F., Galal, A., El-Gohary, A.R.M. (2018). Nano-perovskite decorated carbon nanotubes composite for ultrasensitive determination of a cardio-stimulator drug. Journal of Electroanalytical Chemistry, 816, 149-159. [CrossRef]
  • 17. Atta, N.F., Galal, A., Ahmed, Y.M., El-Ads, E.H. (2019). Design strategy and preparation of a conductive layered electrochemical sensor for simultaneous determination of ascorbic acid, dobutamine, acetaminophen and amlodipine. Sensors and Actuators B: Chemical, 297, 126648. [CrossRef]
  • 18. Ibrahim, H., Temerk, Y. (2020). Synergistic electrocatalytic activity of In2O3@FMWCNTs nanocomposite for electrochemical quantification of dobutamine in clinical patient blood and in injection dosage form. Talanta, 208, 120362. [CrossRef]
  • 19. Chowdhury, R., Maranas, C.D. (2020). From directed evolution to computational enzyme engineering-A review. AIChE Journal, 66(3), e16847. [CrossRef]
  • 20. Chen, L., Wang, X., Lu, W., Wu, X., Li, J. (2016). Molecular imprinting: Perspectives and applications. Chemical Society Reviews, 45(8), 2137-2211. [CrossRef]
  • 21. Uzun, L., Turner, A.P.F. (2016). Molecularly-imprinted polymer sensors: Realising their potential. Biosens Bioelectron, 76, 131-144. [CrossRef]
  • 22. European Medicines Agency. (1995). Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonisation (ICH) Guideline ICH Topic.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılıkta Analitik Kimya
Bölüm Araştırma Makalesi
Yazarlar

Göksu Özçelikay Akyıldız 0000-0001-7919-3236

Sariye İrem Kaya 0000-0003-0578-5399

Erken Görünüm Tarihi 14 Ağustos 2024
Yayımlanma Tarihi 10 Eylül 2024
Gönderilme Tarihi 25 Haziran 2024
Kabul Tarihi 8 Ağustos 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 48 Sayı: 3

Kaynak Göster

APA Özçelikay Akyıldız, G., & Kaya, S. İ. (2024). THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE. Journal of Faculty of Pharmacy of Ankara University, 48(3), 1098-1109. https://doi.org/10.33483/jfpau.1505078
AMA Özçelikay Akyıldız G, Kaya Sİ. THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE. Ankara Ecz. Fak. Derg. Eylül 2024;48(3):1098-1109. doi:10.33483/jfpau.1505078
Chicago Özçelikay Akyıldız, Göksu, ve Sariye İrem Kaya. “THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE”. Journal of Faculty of Pharmacy of Ankara University 48, sy. 3 (Eylül 2024): 1098-1109. https://doi.org/10.33483/jfpau.1505078.
EndNote Özçelikay Akyıldız G, Kaya Sİ (01 Eylül 2024) THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE. Journal of Faculty of Pharmacy of Ankara University 48 3 1098–1109.
IEEE G. Özçelikay Akyıldız ve S. İ. Kaya, “THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE”, Ankara Ecz. Fak. Derg., c. 48, sy. 3, ss. 1098–1109, 2024, doi: 10.33483/jfpau.1505078.
ISNAD Özçelikay Akyıldız, Göksu - Kaya, Sariye İrem. “THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE”. Journal of Faculty of Pharmacy of Ankara University 48/3 (Eylül 2024), 1098-1109. https://doi.org/10.33483/jfpau.1505078.
JAMA Özçelikay Akyıldız G, Kaya Sİ. THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE. Ankara Ecz. Fak. Derg. 2024;48:1098–1109.
MLA Özçelikay Akyıldız, Göksu ve Sariye İrem Kaya. “THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE”. Journal of Faculty of Pharmacy of Ankara University, c. 48, sy. 3, 2024, ss. 1098-09, doi:10.33483/jfpau.1505078.
Vancouver Özçelikay Akyıldız G, Kaya Sİ. THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE. Ankara Ecz. Fak. Derg. 2024;48(3):1098-109.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.