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DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE

Yıl 2024, Cilt: 48 Sayı: 3, 1058 - 1067, 10.09.2024
https://doi.org/10.33483/jfpau.1522001

Öz

Objective: A very efficient and sensitive electrochemical technique utilizing differential pulse voltammetry (DPV) at a boron-doped diamond electrode (BDDE) was devised to measure Palbociclib in this study.
Material and Method: All experiments employed typical three-electrode cell of 10 ml capacity in conjunction with boron-doped diamond electrode, a platinum wire counter electrode, and an Ag/AgCl reference electrode. During electrochemical measurements, DPV and cyclic voltammetry (CV) methods was utilized at BDDE.
Result and Discussion: Based on experimental findings from electrochemical characterization investigations, it was determined that oxidation behavior of Palbociclib in BDDE is irreversible and regulated by diffusion. Anodic peak current exhibited a linear relationship within concentration range of 0.01–1 µM, 0.02–0.8 µM, and 0.02–0.8 µM in pH 2.0 phosphate buffer solution (PBS) for reference substance solution, human serum, and urine samples, respectively. Limits of detection were found as 2.28 nM, 2.93 nM, and 1.31 nM for standard drug solution, human serum and urine samples, respectively. In order to validate the developed method, its repeatability, reproducibility, selectivity, precision and accuracy in all environments were investigated and calculated. This method was successfully applied for the analysis of Palbociclib in human serum and urine samples .

Kaynakça

  • 1. Obeagu, E.I., Obeagu, G.U. (2024). Breast cancer: A review of risk factors and diagnosis. Medicine (United States), 103(3), E36905. [CrossRef]
  • 2. Abhisheka, B., Biswas, S.K., Purkayastha, B. (2023). A comprehensive review on breast cancer detection, classification and segmentation using deep learning. In Archives of Computational Methods in Engineering, Vol. 30, Issue 8, pp. 5023-5052. [CrossRef]
  • 3. Kong, X., Gao, P., Wang, J., Fang, Y., Hwang, K. C. (2023). Advances of medical nanorobots for future cancer treatments. Journal of Hematology and Oncology, 16(1), 1-45. [CrossRef]
  • 4. Dhillon, S. (2015). Palbociclib: First global approval. Drugs, 75(5), 543-551. [CrossRef]
  • 5. Turner, N.C., Ro, J., André, F., Loi, S., Verma, S., Iwata, H., Harbeck, N., Loibl, S., Huang Bartlett, C., Zhang, K., Giorgetti, C., Randolph, S., Koehler, M., Cristofanilli, M. (2015). Palbociclib in hormone-receptor–positive advanced breast cancer. New England Journal of Medicine, 373(3), 209-219 [CrossRef]
  • 6. Cadoo, K.A., Gucalp, A., Traina, T.A. (2014). Palbociclib: An evidence-based review of its potential in the treatment of breast cancer. In Breast Cancer: Targets and Therapy, Vol. 6, pp. 123-133. [CrossRef]
  • 7. Serra, F., Lapidari, P., Quaquarini, E., Tagliaferri, B., Sottotetti, F., Palumbo, R. (2019). Palbociclib in metastatic breast cancer: Current evidence and real-life data. Drugs in Context, 8, 212579. [CrossRef]
  • 8. Turković, L., Bočkor, L., Ekpenyong, O., Silovski, T., Lovrić, M., Crnković, S., Nigović, B., Sertić, M. (2022). Development and validation of a novel LC-MS/MS method for the simultaneous determination of abemaciclib, palbociclib, ribociclib, anastrozole, letrozole, and fulvestrant in plasma samples: A prerequisite for personalized breast cancer treatment. Pharmaceuticals, 15(5), 614. [CrossRef]
  • 9. Poetto, A.S., Posocco, B., Gagno, S., Orleni, M., Zanchetta, M., Iacuzzi, V., Canil, G., Buzzo, M., Montico, M., Guardascione, M., Basile, D., Pelizzari, G., Alberti, M., Gerratana, L., Puglisi, F., Toffoli, G. (2021). A new dried blood spot LC-MS/MS method for therapeutic drug monitoring of palbociclib, ribociclib, and letrozole in patients with cancer. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1185, 122985. [CrossRef]
  • 10. Calucică, D.M., Manda, C.V., Găman, A.M., Răileanu, Ștefan, Stanca, L., Popescu, M.D.E., Mateescu, O. G., Biță, A., Croitoru, O., Neamțu, S.D. (2022). Development of a SPE-LC-MS method for the quantitation of palbociclib and abemaciclib in human plasma. Molecules, 27(23), 8604. [CrossRef]
  • 11. Dange, Y.D., Salunkhe, V.R., Bhinge, S.D., Bhutkar, B.R., Momin, Y.H. (2017). Simultaneous equation method for the estimation of palbociclib and letrozole by uv-visible spectrophotometry. Indian Drugs, 54(09), 61-66. [CrossRef]
  • 12. Mlinarić, Z., Turković, L., Begović, I., Nigović, B., Sertić, M. (2022). Rapid capillary electrophoresis method for simultaneous determination of abemaciclib, ribociclib, and palbociclib in pharmaceutical dosage forms: A green approach. Molecules, 27(21), 7603. [CrossRef]
  • 13. Cheraghi, S., Cetinkaya, A., Kaya, S.I., Atici, E.B., Ozkan, S. A. (2024). Sensitive and selective electrochemical sensor for palbociclib, a highly selective CDK4/6 inhibitor, based on molecularly imprinted polymer. Microchemical Journal, 201, 110689. [CrossRef]
  • 14. Magdy, G., Belal, F., Elmansi, H. (2023). Rapid microwave-assisted synthesis of nitrogen-doped carbon quantum dots as fluorescent nanosensors for the spectrofluorimetric determination of palbociclib: Application for cellular imaging and selective probing in living cancer cells. RSC Advances, 13(7), 4156-4167. [CrossRef]
  • 15. de Jesus, C.S.H., Enache, T.A., Diculescu, V.C. (2020). Charge transfer reaction mechanisms of epoxyketone and boronated peptides at glassy carbon and boron doped diamond electrodes. Journal of Electroanalytical Chemistry, 878, 114733. [CrossRef]
  • 16. Kondo, T. (2021). Recent electroanalytical applications of boron-doped diamond electrodes. Current Opinion in Electrochemistry, 32, 100891. [CrossRef]
  • 17. Dincer, C., Ktaich, R., Laubender, E., Hees, J.J., Kieninger, J., Nebel, C.E., Heinze, J., Urban, G.A. (2015). Nanocrystalline boron-doped diamond nanoelectrode arrays for ultrasensitive dopamine detection. Electrochimica Acta, 185, 101-106. [CrossRef]
  • 18. Altun, Y., Uslu, B., Ozkan, S. A. (2010). Electroanalytical characteristics of lercanidipine and its voltammetric determination in pharmaceuticals and human serum on boron-doped diamond electrode. Analytical Letters, 43(12), 1958-1975. [CrossRef]
  • 19. Cetinkaya, A., Yıldız, E., Kaya, S.I., Çorman, M.E., Uzun, L., Ozkan, S.A. (2022). A green synthesis route to develop molecularly imprinted electrochemical sensor for selective detection of vancomycin from aqueous and serum samples. Green Analytical Chemistry, 2, 100017. [CrossRef]
  • 20. Karadurmus, L., Budak, F., Cetinkaya, A., Atici, E.B., Ozkan, S.A. (2023). Development of highly selective and sensitive molecularly imprinted polymer-based electrochemical sensors for tolvaptan assay in tablets and serum. Analytical Methods, 15(40), 5316-5322. [CrossRef]
  • 21. Uslu, B., Özkan, S.A. (2004). Anodic voltammetry of abacavir and its determination in pharmaceuticals and biological fluids. Electrochimica Acta, 49(25), 4321-4329. [CrossRef]
  • 22. Dogan, B., Tuncel, S., Uslu, B., Özkan, S.A. (2007). Selective electrochemical behavior of highly conductive boron-doped diamond electrodes for fluvastatin sodium oxidation. Diamond and Related Materials, 16(9), 1695-1704. [CrossRef]
  • 23. Yence, M., Cetinkaya, A., Ozcelikay, G., Kaya, S.I., Ozkan, S.A. (2022). Boron-doped diamond Electrodes: recent developments and advances in view of electrochemical drug sensors. Critical Reviews in Analytical Chemistry, 52(5), 1122-1138. [CrossRef]

ANTİ-KANSER İLACI PALBOCICLIB'İN BOR KATKILI ELMAS ELEKTROTTA DİFERANSİYEL PULS VOLTAMETRİ YÖNTEMİ KULLANILARAK İNSAN BİYOLOJİK SIVILARINDAN TAYİNİ

Yıl 2024, Cilt: 48 Sayı: 3, 1058 - 1067, 10.09.2024
https://doi.org/10.33483/jfpau.1522001

Öz

Amaç: Bu çalışmada Palbociclib'i doğru bir şekilde ölçmek için bor katkılı elmas elektrotta (BDDE) diferansiyel puls voltametrisi (DPV) kullanan çok verimli ve hassas bir elektrokimyasal teknik geliştirilmiştir.
Gereç ve Yöntem: Tüm deneylerde bor katkılı elmas elektrot, platin tel karşı elektrot ve Ag/AgCl referans elektrot ile birlikte 10 ml kapasiteli tipik üç elektrotlu hücre kullanılmıştır. Elektrokimyasal ölçümler sırasında BDDE'de DPV ve döngüsel voltametri (CV) yöntemleri kullanılmıştır.
Sonuç ve Tartışma: Elektrokimyasal karakterizasyon incelemelerinden elde edilen deneysel bulgulara dayanarak, Palbociclib'in BDDE'deki oksidasyon davranışının geri dönüşümsüz olduğu ve difüzyonla düzenlendiği belirlenmiştir. Anodik pik akımı, referans madde çözeltisi, insan serumu ve idrar numuneleri için DPV yöntemiyle pH 2.0 fosfat tampon çözeltisinde (PBS) sırasıyla 0.01-1 µM, 0.02-0.8µM ve 0.02-0.8 µM konsantrasyon aralığında doğrusal bir ilişki görülmüştür Tespit limitleri standart ilaç çözeltisi, insan serumu ve idrar numuneleri için sırasıyla 2.28 nM, 2.93 nM ve 1.31 nM olarak bulunmuştur. Geliştirilen yöntemin validasyonu için tekrarlanabilirliği, tekrar üretilebilirliği, seçiciliği, kesinliği ve doğruluğu araştırılmış ve hesaplanmıştır. Bu yöntem insan serum ve idrar örneklerinde Palbociclib analizi için başarıyla uygulanmıştır.

Kaynakça

  • 1. Obeagu, E.I., Obeagu, G.U. (2024). Breast cancer: A review of risk factors and diagnosis. Medicine (United States), 103(3), E36905. [CrossRef]
  • 2. Abhisheka, B., Biswas, S.K., Purkayastha, B. (2023). A comprehensive review on breast cancer detection, classification and segmentation using deep learning. In Archives of Computational Methods in Engineering, Vol. 30, Issue 8, pp. 5023-5052. [CrossRef]
  • 3. Kong, X., Gao, P., Wang, J., Fang, Y., Hwang, K. C. (2023). Advances of medical nanorobots for future cancer treatments. Journal of Hematology and Oncology, 16(1), 1-45. [CrossRef]
  • 4. Dhillon, S. (2015). Palbociclib: First global approval. Drugs, 75(5), 543-551. [CrossRef]
  • 5. Turner, N.C., Ro, J., André, F., Loi, S., Verma, S., Iwata, H., Harbeck, N., Loibl, S., Huang Bartlett, C., Zhang, K., Giorgetti, C., Randolph, S., Koehler, M., Cristofanilli, M. (2015). Palbociclib in hormone-receptor–positive advanced breast cancer. New England Journal of Medicine, 373(3), 209-219 [CrossRef]
  • 6. Cadoo, K.A., Gucalp, A., Traina, T.A. (2014). Palbociclib: An evidence-based review of its potential in the treatment of breast cancer. In Breast Cancer: Targets and Therapy, Vol. 6, pp. 123-133. [CrossRef]
  • 7. Serra, F., Lapidari, P., Quaquarini, E., Tagliaferri, B., Sottotetti, F., Palumbo, R. (2019). Palbociclib in metastatic breast cancer: Current evidence and real-life data. Drugs in Context, 8, 212579. [CrossRef]
  • 8. Turković, L., Bočkor, L., Ekpenyong, O., Silovski, T., Lovrić, M., Crnković, S., Nigović, B., Sertić, M. (2022). Development and validation of a novel LC-MS/MS method for the simultaneous determination of abemaciclib, palbociclib, ribociclib, anastrozole, letrozole, and fulvestrant in plasma samples: A prerequisite for personalized breast cancer treatment. Pharmaceuticals, 15(5), 614. [CrossRef]
  • 9. Poetto, A.S., Posocco, B., Gagno, S., Orleni, M., Zanchetta, M., Iacuzzi, V., Canil, G., Buzzo, M., Montico, M., Guardascione, M., Basile, D., Pelizzari, G., Alberti, M., Gerratana, L., Puglisi, F., Toffoli, G. (2021). A new dried blood spot LC-MS/MS method for therapeutic drug monitoring of palbociclib, ribociclib, and letrozole in patients with cancer. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1185, 122985. [CrossRef]
  • 10. Calucică, D.M., Manda, C.V., Găman, A.M., Răileanu, Ștefan, Stanca, L., Popescu, M.D.E., Mateescu, O. G., Biță, A., Croitoru, O., Neamțu, S.D. (2022). Development of a SPE-LC-MS method for the quantitation of palbociclib and abemaciclib in human plasma. Molecules, 27(23), 8604. [CrossRef]
  • 11. Dange, Y.D., Salunkhe, V.R., Bhinge, S.D., Bhutkar, B.R., Momin, Y.H. (2017). Simultaneous equation method for the estimation of palbociclib and letrozole by uv-visible spectrophotometry. Indian Drugs, 54(09), 61-66. [CrossRef]
  • 12. Mlinarić, Z., Turković, L., Begović, I., Nigović, B., Sertić, M. (2022). Rapid capillary electrophoresis method for simultaneous determination of abemaciclib, ribociclib, and palbociclib in pharmaceutical dosage forms: A green approach. Molecules, 27(21), 7603. [CrossRef]
  • 13. Cheraghi, S., Cetinkaya, A., Kaya, S.I., Atici, E.B., Ozkan, S. A. (2024). Sensitive and selective electrochemical sensor for palbociclib, a highly selective CDK4/6 inhibitor, based on molecularly imprinted polymer. Microchemical Journal, 201, 110689. [CrossRef]
  • 14. Magdy, G., Belal, F., Elmansi, H. (2023). Rapid microwave-assisted synthesis of nitrogen-doped carbon quantum dots as fluorescent nanosensors for the spectrofluorimetric determination of palbociclib: Application for cellular imaging and selective probing in living cancer cells. RSC Advances, 13(7), 4156-4167. [CrossRef]
  • 15. de Jesus, C.S.H., Enache, T.A., Diculescu, V.C. (2020). Charge transfer reaction mechanisms of epoxyketone and boronated peptides at glassy carbon and boron doped diamond electrodes. Journal of Electroanalytical Chemistry, 878, 114733. [CrossRef]
  • 16. Kondo, T. (2021). Recent electroanalytical applications of boron-doped diamond electrodes. Current Opinion in Electrochemistry, 32, 100891. [CrossRef]
  • 17. Dincer, C., Ktaich, R., Laubender, E., Hees, J.J., Kieninger, J., Nebel, C.E., Heinze, J., Urban, G.A. (2015). Nanocrystalline boron-doped diamond nanoelectrode arrays for ultrasensitive dopamine detection. Electrochimica Acta, 185, 101-106. [CrossRef]
  • 18. Altun, Y., Uslu, B., Ozkan, S. A. (2010). Electroanalytical characteristics of lercanidipine and its voltammetric determination in pharmaceuticals and human serum on boron-doped diamond electrode. Analytical Letters, 43(12), 1958-1975. [CrossRef]
  • 19. Cetinkaya, A., Yıldız, E., Kaya, S.I., Çorman, M.E., Uzun, L., Ozkan, S.A. (2022). A green synthesis route to develop molecularly imprinted electrochemical sensor for selective detection of vancomycin from aqueous and serum samples. Green Analytical Chemistry, 2, 100017. [CrossRef]
  • 20. Karadurmus, L., Budak, F., Cetinkaya, A., Atici, E.B., Ozkan, S.A. (2023). Development of highly selective and sensitive molecularly imprinted polymer-based electrochemical sensors for tolvaptan assay in tablets and serum. Analytical Methods, 15(40), 5316-5322. [CrossRef]
  • 21. Uslu, B., Özkan, S.A. (2004). Anodic voltammetry of abacavir and its determination in pharmaceuticals and biological fluids. Electrochimica Acta, 49(25), 4321-4329. [CrossRef]
  • 22. Dogan, B., Tuncel, S., Uslu, B., Özkan, S.A. (2007). Selective electrochemical behavior of highly conductive boron-doped diamond electrodes for fluvastatin sodium oxidation. Diamond and Related Materials, 16(9), 1695-1704. [CrossRef]
  • 23. Yence, M., Cetinkaya, A., Ozcelikay, G., Kaya, S.I., Ozkan, S.A. (2022). Boron-doped diamond Electrodes: recent developments and advances in view of electrochemical drug sensors. Critical Reviews in Analytical Chemistry, 52(5), 1122-1138. [CrossRef]
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılıkta Analitik Kimya
Bölüm Araştırma Makalesi
Yazarlar

Melike Akan 0009-0002-3555-4532

Çiğdem Kanbeş Dindar 0000-0002-3557-5029

Nazife Aslan 0000-0002-2622-5908

Bengi Uslu 0000-0002-7327-4913

Erken Görünüm Tarihi 11 Ağustos 2024
Yayımlanma Tarihi 10 Eylül 2024
Gönderilme Tarihi 24 Temmuz 2024
Kabul Tarihi 3 Ağustos 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 48 Sayı: 3

Kaynak Göster

APA Akan, M., Kanbeş Dindar, Ç., Aslan, N., Uslu, B. (2024). DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE. Journal of Faculty of Pharmacy of Ankara University, 48(3), 1058-1067. https://doi.org/10.33483/jfpau.1522001
AMA Akan M, Kanbeş Dindar Ç, Aslan N, Uslu B. DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE. Ankara Ecz. Fak. Derg. Eylül 2024;48(3):1058-1067. doi:10.33483/jfpau.1522001
Chicago Akan, Melike, Çiğdem Kanbeş Dindar, Nazife Aslan, ve Bengi Uslu. “DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE”. Journal of Faculty of Pharmacy of Ankara University 48, sy. 3 (Eylül 2024): 1058-67. https://doi.org/10.33483/jfpau.1522001.
EndNote Akan M, Kanbeş Dindar Ç, Aslan N, Uslu B (01 Eylül 2024) DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE. Journal of Faculty of Pharmacy of Ankara University 48 3 1058–1067.
IEEE M. Akan, Ç. Kanbeş Dindar, N. Aslan, ve B. Uslu, “DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE”, Ankara Ecz. Fak. Derg., c. 48, sy. 3, ss. 1058–1067, 2024, doi: 10.33483/jfpau.1522001.
ISNAD Akan, Melike vd. “DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE”. Journal of Faculty of Pharmacy of Ankara University 48/3 (Eylül 2024), 1058-1067. https://doi.org/10.33483/jfpau.1522001.
JAMA Akan M, Kanbeş Dindar Ç, Aslan N, Uslu B. DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE. Ankara Ecz. Fak. Derg. 2024;48:1058–1067.
MLA Akan, Melike vd. “DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE”. Journal of Faculty of Pharmacy of Ankara University, c. 48, sy. 3, 2024, ss. 1058-67, doi:10.33483/jfpau.1522001.
Vancouver Akan M, Kanbeş Dindar Ç, Aslan N, Uslu B. DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE. Ankara Ecz. Fak. Derg. 2024;48(3):1058-67.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.