FATTY ACID COMPOSITION AND BIOLOGICAL ACTIVITIES OF VITIS VINIFERA L. (ANTEP KARASI) SEED OIL

Yıl 2025, Cilt: 49 Sayı: 4-14th International Symposium on Pharmaceutical Sciences (ISOPS-14), 4 - 4

Kübra Öğüt , Gülmira Özek , Pervin Soyer , Temel Özek

Öz

Objective: This study examined the chemical composition and biological activity of Vitis vinifera L. (Antep Karası) seed oil, focusing on its fatty acid profile, phenolic content, and various antioxidant and antimicrobial properties.
Material and Method: Seeds of V. vinifera (known as “Antep Karası”) were collected from vines in Gaziantep, Türkiye, and the seed oil was extracted using n-hexane in a Soxhlet apparatus. Chemical composition of the oil was studied using GC-FID/MS after transesterification of fatty acids with BF₃ reagent. Standard spectrophotometric and microbiological tests were used to measure total flavonoids, phenolics, antioxidant capacity, enzyme inhibition, and antimicrobial, antifungal, and antibiofilm activities.
Result and Discussion: V. vinifera (Antep Karası) seeds yielded 8.2% oil. Total phenolic and flavonoid content was 0.074 ± 0.001 mg GAE/gseed oil and 0.023 ± 0.001 mg RE/gseed oil. DPPH radical scavenging (IC₅₀ = 1.423 ± 0.004 mg/ml), β-carotene bleaching inhibition (0.182 ± 0.020 mg/ml), and TEAC value (0.012 µmol trolox/mgseed oil) were determined. The antibacterial evaluation revealed that the MBC values varied from 7500 -15000 µg/ml. Additionally, antifungal activity by MFC was observed with an MFC value of 7500 µg/ml. The antibiofilm assay revealed an inhibition activity in a dose-dependent manner. Even at a concentration of 1875 µg/ml, biofilm inhibition of 50% and above was observed.

Anahtar Kelimeler

Antep karası , biological activities , fatty acid composition , GC-MS/FID , Vitis vinifera L.

Etik Beyan

This study does not include any human or animal experiments; therefore, ethics committee approval was not required.

Destekleyen Kurum

This research received no external funding

Teşekkür

The authors declare that no acknowledgments are applicable.

VITIS VINIFERA L. (ANTEP KARASI) YAĞININ YAĞ ASİDİ BİLEŞİMİ VE BİYOLOJİK AKTİVİTELERİ

Öz

Amaç: Bu çalışma, Vitis vinifera L. (Antep Karası) çekirdek yağının kimyasal bileşimini ve biyolojik aktivitesini incelemiş, özellikle yağ asidi profiline, fenolik içeriğine ve çeşitli antioksidan ve antimikrobiyal potansiyeline odaklanmıştır.
Gereç ve Yöntem: Türkiye'nin Gaziantep ilindeki asmalardan toplanan V. vinifera (Antep Karası) çekirdeklerinden, n-hekzan kullanılarak Soxhlet cihazında yağ ekstraksiyonu gerçekleştirilmiştir. Yağ asitlerinin BF₃ reaktifi ile transesterifikasyonundan sonra, yağın kimyasal bileşimi GK-KS/AİD kullanılarak incelenmiştir. Toplam flavonoitler, fenolikler, antioksidan kapasite, enzim inhibisyonu ve antimikrobiyal, antifungal ve antibiyofilm aktivitelerini ölçmek için standart spektrofotometrik ve mikrobiyolojik testler kullanılmıştır.
Sonuç ve Tartışma: V. vinifera (Antep Karası) çekirdeklerinden %8.2 oranında yağ elde edilmiştir. Toplam fenolik ve flavonoid miktarları sırasıyla 0.074 ± 0.001 mg GAE/gçekirdek yağı ve 0.023 ± 0.001 mg RE/gçekirdek yağı olarak belirlenmiştir. DPPH radikal süpürme (IC₅₀ = 1.423 ± 0.004 mg/ml), β-karoten ağarması inhibisyonu (0.182 ± 0.020 mg/ml) ve TEAC değeri (0.012 µmol trolox/mgçekirdek yağı) belirlenmiştir. Antibakteriyel değerlendirme, MBC değerlerinin 7500 - 15000 µg/ml aralığında değiştiği saptanmıştır. Ayrıca, MFC ile antifungal aktivite değeri 7500 µg/ml olarak belirlenmiştir. Antibiyofilm testi, doz bağımlı inhibisyon etkisi göstermiştir; 1875 µg/ml konsantrasyonda bile %50 ve üzeri biyofilm inhibisyonu gözlenmiştir.

Anahtar Kelimeler

Antep karası , biyolojik aktiviteler , GK-KS/AİD , Vitis vinifera L. , yağ asidi kompozisyonu

Etik Beyan

Bu çalışma herhangi bir insan veya hayvan deneyi içermemektedir; bu nedenle etik kurul onayı gerekmemiştir.

Destekleyen Kurum

Bu çalışma herhangi bir kurum/kuruluş tarafından desteklenmemiştir.

Teşekkür

Yazarların teşekkür beyanı bulunmamaktadır.

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