Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women

Öz

Background: To compare the serum vimentin levels between the pregnant women diagnosed with preeclampsia (PE) and the normal pregnant women. Materials and Methods: This sectional study was conducted on 40 pregnant women diagnosed with preeclampsia and 21 healthy pregnant women. The maternal serum vimentin levels were evaluated via the sandwich enzyme immunoassay method. Results: The mean vimentin levels were found significantly different between the groups. (47.25±29.74 vs.19.39±3.76 p=0.000) No significant difference was discovered between the two groups in respect of the gestational week, platelet level, parity and body mass index (BMI) during the diagnosis (p>0.05). Conclusion: Findings demonstrate that vimentin plays a role in the pathogenesis of preeclampsia. However, further studies, in which the number of patients is high and subgroups are compared, are needed.

Anahtar Kelimeler

• preeclampsia
• vimentin
• pregnancy
• hypertension

Preeklamptik ve Normotansif Gebeler Arasındaki Vimentin Seviyelerinin Karşılaştırılması

Öz

Amaç: Preeklampsi (PE) tanısı alan gebeler ile normal gebeler arasındaki serum vimentin seviyelerini karşılaştırmaktır. Materyal Method: Kesitsel tipteki bu çalışmada 40 preeklampsi tanısı alan gebe kadın ve 21 sağlıklı gebe üzerinde gerçekleştirilmiştir. Maternal serum vimentin seviyeleri sandwich enzim immunoassay yöntemi ile değerlendirilmiştir. Sonuç: Ortalama vimentin düzeyleri gruplar arasında anlamlı olarak farklı saptandı. (47,25±29,74 vs.19,39±3,76 p=0,000) Tanı esnasındaki gebelik haftası, trombosit seviyesi, parite ve vücut kitle indeksi (VKİ) açısından iki grup arasında anlamlı farklılık saptanmadı (p>0.05). Tartışma: Bulgular vimentinin preeklampsinin patogenezinde rol oynadığını göstermektedir. Ancak hasta sayısı yüksek ve subgrupların karşılaştırıldığı daha ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• preeklampsi
• vimentin
• gebelik
• hipertansiyon

Kaynakça

1. References 1. Yuksel MA, Tuten A, Oncul M, Acikgoz AS, Temel Yuksel I et.al Serum endocan concentration in women with pre-eclampsia. Arch Gynecol Obstet. 2015 Jul;292(1):69-73. 2. Akbas M, Koyuncu FM, Artunc-Ulkumen B, Taneli F, Ozdemir H. Maternal serum perlecan levels in women with preeclampsia. Hypertens Pregnancy .2020 Feb;39(1):70-76. 3. von Dadelszen P, Payne B, Li J, et al. Prediction of adverse maternal outcomes in pre-eclampsia: development and validation of the full PIERS model. PIERS Study Group. Lancet. 2011;377:219–227. 4. Roberts JM, Lain KY (2002) Recent insights into the pathogenesis of pre-eclampsia. Placenta 23(5):359–372. 5. Mary S, Kulkarni MJ, Mehendale SS, Joshi SR, Giri AP. Differential accumulation of vimentin fragments in preeclamptic placenta. Cytoskeleton (Hoboken) 2017 Nov;74(11):420-425. 6. Lambert G, Brichant JF, Hartstein G, Bonhomme V, Dewandre PY. Preeclampsia: an update. Acta Anaesthesiol Belg. 2014;65(4):137-49. 7. Zergeroğlu S, Mollamahmutoğlu L, Reyhan H, Aydoğdu T. ‘Endometrial ve endoservikal adenokarsinomların ayırıcı tanısında vimentin pozitifliğinin değeri’ Türk Jinekolojik Onkoloji Dergisi Ekim 2006, Cilt 9, Say› 4, Sayfa 97-100. 8. Du L, Kuang L, He F, Tang W, Sun W, Chen D. Mesenchymal-to-epithelial transition in the placental tissues of patients with preeclampsia. Hypertens Res. 2017 Jan;40(1):67-72. 9. Mendez, M. G., Kojima, S., & Goldman, R. D. (2010). Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. Faseb Journal, 24, 1838– 1851. 10. Kokkinos MI, Murthi P, Wafai R, Thompson EW, Newgreen DF. Cadherins in the human placenta—epithelial–mesenchymal transition (EMT) and placental development. Placenta 2010; 31: 747-755. 11. Sevgi İrtegün, Elif Ağaçayak, Engin Deveci. Preeklamptik ve normotansif plasentalarda VEGF ve Vimentin ekspresyon düzeylerinin immunohistokimya ve Western Blot yöntemleri ile incelenmesi Dicle Medical Journal 2016; 43 (3): 400-405. 12. Woolf EC, Curley KL, Liu Q, et al. The ketogenic diet alters the hypoxic response and affects expression of proteins associated with angiogenesis, invasive potential and vascular permeability in a mouse glioma model. PLoS ONE 2015;10: e0130357. doi:10.1371/journal.pone.0130357. 13. Shirakawa T, Miyahara Y, Kenji Tanimura K, et al. Expression of Epithelial-Mesenchymal Transition-related Factors in Adherent Placenta. Int J Gynecol Pathol 2015;34:584–9. 14. Granger, J. P.; Alexander, B. T.; Llinas, M. T.; Bennett, W. A.; Khalil, R. A. Pathophysiology of hypertension during preeclampsia linking placental ischemia with endothelial dysfunction. Hypertension 2001, 38, 718−22. 15. Andrade-Scherholz PL, Cristina de Souza P, Spadacci-Morena DD, Godosevicius Katz S. Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells. Placenta 2003;34:518–25. 16. M.E. Sak, E. Deveci, A. Turgut, S. Sak, M.S. Evsen, T. Gul, S. Kalkanlı. ‘Placental expression of vimentin, desmin and ultrastructural changes in the villi in patients with HELLP syndrome’ European Review for Medical and Pharmacological Sciences 2013;17:874-878.