Toplanan Oosit Sayısı ve Olgun Oosit Oranı IVF Sonuçlarını Etkiler mi?

Yıl 2023, Cilt: 20 Sayı: 1, 1596 - 1602, 30.03.2023

Mehmet Caner Özer , Ayten Türkkanı , Derya Özdemir Taş , Şebnem Özyer , Mustafa Turan , Nafiye Yılmaz , Özlem Moraloğlu Tekin

https://doi.org/10.38136/jgon.1176830

Öz

Amaç: Bu çalışmanın amacı, tek fresh embriyo transferinde elde edilen oosit sayısı ve olgun oosit oranının IVF sonuçları üzerindeki etkilerini araştırmaktır.
Gereç ve Yöntemler: Toplanan oosit sayısı ve matür oosit oranı açısından iki ana grup oluşturuldu. 561 IVF siklusunda elde edilen oosit sayısına göre 0-5 (n=175) oosit grup 1, 6-10 (n=214) oosit grup 2, 11-15 (n=121) oosit grup 3 olarak belirlendi. grup 3 ve grup 4 olarak 16 veya daha fazla oosit (n=51). Matür oosit (metafaz II, MII oosit) oranına göre grup 1A (n=338) %76-100 matür oosit, grup 2A (n=150) %75-50 matür oosit ve grup 3A (n) olmak üzere üç grup oluşturuldu. =73) %50'den az olgun oosit.
Bulgular: Toplanan oosit sayısı ile fertilizasyon oranı negatif korelasyon gösterirken, 6-10 oosit grubunda Β-hCG pozitifliği ve klinik gebelik oranlarının daha iyi olduğu gözlendi. Ortalama yaş, bazal LH ve ovulasyonun tetiklendiği gün E2 seviyeleri ve fertilizasyon oranı, üç olgun oosit oranı grubu arasında önemli ölçüde farklıydı.
Sonuç: Sonuç olarak, toplanan oosit sayısından bağımsız olarak kohorttaki MII oosit oranları IVF sonuçlarını etkilemedi.

Anahtar Kelimeler

Oosit sayısı, Olgun oosit oranı, Fresh tek embriyo transferi

Do the Number of Oocytes Retrieved and Mature Oocyte Rate in the Cohort Affect IVF Results?

Öz

Aim: The aim of this study was to investigate the effects of the number of oocytes retrieved and rate of mature oocytes on IVF outcomes in single fresh embryo transfer.
Materials and Medhods: Two main groups were formed regarding number of oocytes retrieved and mature oocyte rate. According to the number of oocytes retrieved in 561 IVF cycles, 0-5 (n=175) oocytes were determined as group 1, 6-10 (n=214) oocytes as group 2, 11-15 (n=121) oocytes as group 3 and 16 or more oocytes as group 4 (n=51). Regarding mature oocyte (metaphase II, MII oocyte) rate, three groups were formed: group 1A (n=338) 76-100% mature oocytes, group 2A (n=150) 75-50% mature oocytes, and group 3A (n=73) less than 50% mature oocytes.
Results: The number of oocytes retrieved was negatively correlated fertilization rate, whereas Β-hCG positivity and clinical pregnancy rates were observed to be better in the 6-10 oocytes group. The mean age, basal LH, and ovulation trigger day E2 levels, and fertilization rate were differed significantly between three groups of mature oocyte rate.
Conclusion: In conclusion, MII oocyte rates in the cohort, regardless of the number of oocytes retrieved, did not affect IVF outcomes.

Anahtar Kelimeler

Number of oocyte, Mature oocyte rate, Fresh single embryo transfer.

Kaynakça

• 1. Haahr, T., Esteves, S. C., Humaidan, P. 2018. Individualized controlled ovarian stimulation in expected poor-responders: an update. Reprod Biol Endocrinol, 16, 20.
• 2. Baker, V.L., Brown, M.B., Luke, B., Conrad, K.P. 2015. Association of number of retrieved oocytes with live birth rate and birth weight: an analysis of 231,815 cycles of in vitro fertilization. Fertil. Steril. 103: 931–938.e932
• 3. Van der Gaast, M.H., Eijkemans, M.J., van der Net, J.B., de Boer, E.J., Burger, C.W., van Leeuwen, F.E., Fauser, B.C., Macklon, N.S. 2006. Optimum number of oocytes for a successful first IVF treatment cycle. Reprod Biomed Online 13: 476–480.
• 4. Valbuena, D., Martin, J., de Pablo, J.L., Remohi, J., Pellicer, A., Simon, C. 2001. Increasing levels of estradiol are deleterious to embryonic implantation because they directly affect the embryo. Fertil. Steril. 76: 962–968.
• 5. Sesh Kamal Sunkara, Vivian Rittenberg, Nick Raine-Fenning, Siladitya Bhattacharya, Javier Zamora, and Arri Coomarasamy. 2011. Association between the number of eggs and live birth in IVF treatment: an analysis of 400 135 treatment cycles. Human Reproduction Vol.26, No.7 pp. 1768–1774.
• 6. Law YJ, Zhang N, Kolibianakis EM, Costello MF, Keller E, Chambers GM et all, Is there an optimal number of oocytes retrieved at which live birth rates or cumulative live birth rates per aspiration are maximized after ART? A systematic review. Reprod Biomed Online. 2021;42(1):83-104.
• 7. Rubıno, P., Vıgano, P., Luddı, A. & Pıombonı, P. 2016. The ICSI procedure from past to future: a systematic review of the more controversial aspects. Human Reproduction Update, 22, 194-227.
• 8. Beall, S., Brenner, C., Segars, J. 2010. Oocyte maturation failure: A syndrome of bad eggs. Fertility and Sterility, 94, 2507–2513.
• 9. Avci B., Kasapoglu, I., Cakir C., Ozbay, A., Ata, B., Uncu, G. 2020. Fertilisation and early embryonic development of immature and rescue in vitro-matured sibling oocytes. Hum Fertil (Camb). Jan 17;1-10.
• 10. Halvaei, I., Ali Khalili, M., Razi, M. H., Nottola, S. A. 2012. The effect of immature oocytes quantity on the rates of oocytes maturity and morphology, fertilization, and embryo development in ICSI cycles. J Assist Reprod Genet, 29, 803-10.
• 11. Luna Mao, Hangying Lou, Yiyun Lou, Ning Wang, Fan Jin. 2014. Behaviour of cytoplasmic organelles and cytoskeleton during oocyte maturation Reproductive BioMedicine Online 28, 284–299.
• 12. Sen, A., Caiazza, F. 2013. Oocyte maturation: a story of arrest and release. Front Biosci (Schol Ed), 5, 451-77.
• 13. Alessandra P., Mohamad I., Derek K., Stephen C., Zev R., Gianpiero D. Palermo. 2019. High proportion of immature oocytes in a cohort reduces fertilization, embryo development, pregnancy and live birth rates following ICSI Reprod Biomed Online Oct;39(4):580-587.
• 14. Reichman, D. E., Politch, J., Ginsburg, E. S., Racowsky, C. 2010. Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential, embryo development, and reproductive outcomes. J Assist Reprod Genet, 27, 347-56.
• 15. ESHRE Special Interest Group of Embryology and Alpha Scientists in Reproductive Medicine. 2017. The Vienna consensus: report of an expert meeting on the development of art laboratory performance indicators. Human Reproduction Open, pp. 1–17.
• 16. Tesarik J., Greco E. 1999. The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear stage morphology. Human Reproduction 14(5): 1318-23.
• 17. Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. 2011. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Human Reproduction, Vol.26, No.6 pp. 1270–1283, 2011
• 18. Baczkowski T, Kurzawa R, Głabowski W. Methods of embryo scoring in in vitro fertilization. Reprod Biol 2004;4:5–22.
• 19. Mitwally, M.F., Bhakoo, H.S., Crickard, K., Sullivan, M.W., Batt, R.E., Yeh, J. 2006. Estradiol production during controlled ovarian hyperstimulation correlates with treatment outcome in women undergoing in vitro fertilization–embryo transfer. Fertility and sterility; 86: 588–596.
• 20. Kok, J.D., Looman, C.W., Weima, S.M., Te Velde, E.R. 2006. A high number of oocytes obtained after ovarian hyperstimulation for in vitro fertilization or intracytoplasmic sperm injection is not associated with decreased pregnancy outcome. Fertility Sterility; 85: 918–924
• 21. Fatemi, H.M., Doody, K., Griesinger, G., Witjes, H., Mannaerts, B. 2013. High ovarian response does not jeopardize ongoing pregnancy rates and increases cumulative pregnancy rates in a GnRH-antagonist protocol. Human Reproduction; 28: 442–452.
• 22. Briggs, R., Kovacs, G., Maclachlan, V., Motteram, C., Baker, H.W. 2015. Can you ever collect too many oocytes? Human Reproduction 30: 81–87.
• 23. Venetis, C., Kolibianakis, E., Bosdou, J., Tarlatzis, B. 2013. Progesterone elevation and probability of pregnancy after IVF: a systematic review and meta-analysis of over 60 000 cycles. Human Reproduction Update 19: 433–457.
• 24. Conti, M., Franciosi, F. 2018. Acquisition of oocyte competence to develop as an embryo: integrated nuclear and cytoplasmic events. Human Reproduction Update, 24, 245-266.
• 25. Reader, K. L., Stanton, J.-A. L., Juengel, J. L. 2017. The Role of Oocyte Organelles in Determining Developmental Competence. Biology (Basel). Sep 18;6(3):35.
• 26. Sathananthan, A.H., Selvaraj, K., Girijashankar, M.L., Ganesh, V., Selvaraj, P., Trounson, A.O. 2006. From oogonia to mature oocytes: Inactivation of the maternal centrosome in humans. Microsc. Res. Tech.; 69: 396–407.
• 27. Arroyo, A., Kim, B., Yeh, J. 2020. Luteinizing Hormone Action in Human Oocyte Maturation and Quality: Signaling Pathways, Regulation, and Clinical Impact. Reproductive Sciences 27, 1223-1252.
• 28. Livia Z. Yanez, Jinnuo Han, Barry B. Behr, Renee A. Reijo Pera, David B. Camarillo. 2016. Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization Nature Communications DOI: 10.1038/Ncomms10809 1-12
• 29. Balakier, H., Sojecki, A., Motamedi, G., Librach, C. 2004. Time dependent capability of human oocytes for activation and pronuclear formation during metaphase II arrest. Hum Reprod, 19, 982-7.
• 30. Labarta, E., Bosch, E., Mercader, A., Alama, P., Mateu, E., Pellicer, A. 2017. A Higher Ovarian Response after Stimulation for IVF Is Related to a Higher Number of Euploid Embryos. Biomed. Res. Int. 2017:5637923.
• 31. Vermey BG, Chua SJ, Zafarmand MH, Wang R, Longobardi S, Cottell E, Beckers F, Mol BW, Venetis CA, D'Hooghe 2019. Is there an association between oocyte number and embryo quality? A systematic review and meta-analysis. T.Reprod Biomed Online. Nov;39(5):751-763.
• 32. Vollenhoven B, Hunt S. Ovarian ageing and the impact on female fertility, F1000Research, 7, F1000Faculty Rev, 2018: 1835