Do midkine levels in serum and follicular fluid affect IVF-ICSI outcome?

Abstract

Objective: Current studies have demonstrated that midkine (MK) inhibits the apoptosis in granulosa cells and is responsible for follicular angiogenesis. The aim of this study was to investigate the relation of MK levels and ART outcome.
Material and Method: The study included a total of 99 patients who underwent IVF-ICSI at Ondokuz Mayis University between February 2019 and April 2019. Of 99 patients, no embryo development was formed in 1 patient, no oocytes were obtained by oocyte pick up (OPU) in 14 patients and the oocyte cryopreservation was established in 17 patients. In total, 67 patients underwent fresh embryo transfer after IVF-ICSI. On the day of OPU, the level of midkine in serum and follicular fluids were examined. The primary outcome was determined as pregnancy and secondary outcome was determined as blastocyst‐stage embryos.
Results: The level of midkine in serum and follicular fluid was found to be higher in pregnant women compared with non-pregnant women (p=0.042 and p=0.01 respectively). Midkine levels in follicular fluid and serum lead to an increase in blastocyst development.
Conclusion: Midkine levels in follicular fluid and serum may lead to an increase in blastocyst development. The level of midkine is higher in pregnant subjects than in non-pregnant subjects.

Keywords

• in vitro fertilization,pregnancy,blastocyst,midkine

Serum ve foliküler sıvıdaki midkin düzeyleri IVF-ICSI sonuçlarını etkiler mi?

Abstract

Amaç: Güncel çalışmalar, midkinin (MK) granüloza hücrelerinde apoptozu inhibe ettiğini ve foliküler anjiyogenezden sorumlu olduğunu göstermiştir. Bu çalışmanın amacı, midkin seviyeleri ile ART sonuçları arasındaki ilişkiyi araştırmaktır.
Gereç ve Yöntem: Çalışmaya Şubat 2019 ve Nisan 2019 tarihleri arasında Ondokuz Mayıs Üniversitesi’nde IVF-ICSI uygulanan toplam 99 hasta dahil edildi. Çalışmadaki 99 hastanın birinde embriyo gelişimi sağlanamadı ve 14 hastada oosit toplanmasıyla (OPU) oosit elde edilmedi. ve oosit kriyoprezervasyon 17 hastada oluşturuldu. Toplam 67 hastaya IVF-ICSI sonrası taze embriyo transferi yapıldı. OPU gününde serum ve foliküler sıvılardaki midkin seviyesi incelendi. Primer sonuç gebelik, sekonder sonuç blastosist-aşamalı embriyolar olarak belirlendi.
Bulgular: Serum ve foliküler sıvıdaki midkin düzeyi gebelerde gebe olmayanlara göre daha yüksek bulundu (p=0,042, p=0,01). Foliküler sıvı ve serumdaki midkin seviyeleri blastokist gelişiminde artışa neden olduğu görülmüştür.
Sonuç: Foliküler sıvı ve serumdaki midkin seviyeleri blastokist gelişiminde artışa neden olabilir. Midkin seviyesi gebelerde gebe olmayanlara göre daha yüksek bulunmuştur.

Keywords

• İn vitro fertilizasyon,gebelik,blastokist,midkin

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