Araştırma Makalesi
BibTex RIS Kaynak Göster
Yıl 2021, Cilt: 4 Sayı: 1, 33 - 37, 21.01.2021
https://doi.org/10.32322/jhsm.804331

Öz

Destekleyen Kurum

Kırııkale Üniversitesi Bilimsel Araştırma Proje Birimi (BAP)

Proje Numarası

2016/005

Kaynakça

  • Harper D, Young A, McNaught CE. The physiology of wound healing. Surgery (Oxford) 2014; 32: 445-50.
  • Macdonald J, Asiedu K. WAWLC: World Alliance for Wound and Lymphedema Care. Wounds 2010; 22: 55-9.
  • Sayek İ, Ozmen MM, Temel Cerrahi El Kitabı, Guneş Tıp Kitabevleri, Ankara, 2009
  • Coşkun Ö, Uzun G, Dal D ve ark. Kronik yarada tedavi yaklaşımları. Gülhane Tıp Derg 2016; 58: 207-28.
  • Rajpaul K. Biofilm in wound care. Br J Community Nurs; Suppl Wound Care: 2015; 6: 10-1.
  • Broughton G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg 2006; 117: 125-34
  • Jeffcoate J.W, Price P, Harding G.K.Wound healing and treatments for people with diabetic foot ulsers. Dıabetes/Metabolısm Research and Reviews 2004; 20: 78–89.
  • Martino MM, Tortelli F, Mochizuki M, al. Engineering the growth factor microenvironment with fibronectin domains to promote wound and bone tissue healing. Sci Transl Med 2011; 3: 100-89.
  • Rodella LF, Favero G, Boninsegna R, et al. Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction. Microsc Res Tech 2011; 74: 772-7.
  • Chen Y, Cai Z, Zheng D, et al. Inlay osteotome sinus floor elevation with concentrated growth factor application and simultaneous short implant placement in severely atrophic maxilla. Scientific reports 2016; 6: 273-8.
  • Kim T-H, Kim S-H, Sándor GK, Kim Y-D. Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing. Archives of oral biology 2014; 59: 550-8.
  • İnan S, Ozbilgin K. Kok Hucre Biyolojisi. Sağlıkta Birikim. Cilt 1, Sayı 5, 2009: 11-23.
  • Kok IJ, Peter SJ, Archambault M et al. Investigation of allogeneic mesenchymal stem cell-based alveolar bone formation: preliminary findings. Clin Oral Implants Res 2003; 14: 481-9.
  • Öztopalan DF, Işık R, Durmuş AS. Yara iyileşmesinde büyüme faktörleri ve sitokinlerin rolü. Dicle Üniv Vet Fak Derg 2017; 10: 83-8
  • Greenhalgh DG. Wound healing and diabetes mellitus. Clin Plastic Surg 2003; 30: 37-45.
  • Galiano RD, Michaels VJ, Dobryansky M, Levine JP, Gurtner GC. Quantitative and reproducible murine model of excisional wound healing. Wound Repair Regen 2004; 12: 485-92.
  • Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabet Med 2006; 23: 594-608.
  • Larsen JA, Overstreet J. Pulsed radio frequency energy in the treatment of complex diabetic foot wounds: two cases. J Wound Ostomy Continence Nurs 2008; 35: 523-7.
  • Reid RR, Said HK, Mogford JE, Mustoe TA. The future of wound healing: Pursuing surgical models in transgenic and knockout mice. J Am Coll Surg 2004; 199: 578-85.
  • Guo SC, Tao SC, Yin WJ, Qi X, Yuan T, Zhang CQ. Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model. Theranostics 2017; 7: 81-96.
  • Yuan T, Guo SC, Han P, Zhang CQ, Zeng BF. Applications of leukocyte- and platelet-rich plasma (L-PRP) in trauma surgery. Curr Pharm Biotechnol 2012; 13: 1173–84.
  • Wang F, Sun Y, He D, Wang L. Effect of Concentrated Growth Factors on the Repair of the Goat Temporomandibular Joint. J Oral Maxillofac Surg 2017; 75: 498-507.
  • Shih B, Garside E, McGrouther DA, Bayat A. Molecular dissection of abnormal wound healing processes resulting in keloid disease. Wound Repair Regen 2010; 18: 139-53.
  • Ulaşlı AM. Kas iskelet sistemi yaralanmalarında plateletten zengin plazma tedavisi. Kocatepe Tıp Derg 2012: 13; 1-3.
  • Kim JM, Sohn DS, Bae MS, Moon JW, Lee JH, Park IS. Flapless transcrestal sinus augmentation using hydrodynamic piezoelectric internal sinus elevation with autologous concentrated growth factors alone. Implant Dent 2014; 23: 168-74.
  • Sohn D, Moon J, Moon Y, Park J, Jung H. The use of concentrated growth factors (CGF) for sinus augmentation. J Oral Implant 2009; 38: 25-38.
  • Khojasteh A, Eslaminejad MB, Nazarian H. Mesenchymal stem cells enhance bone regeneration in rat calvarial critical size defects more than platelete-rich plasma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106: 356-63.
  • Falanga V, Iwamoto S, Chartier M, et al. Autologous bone marrow-derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue Eng 2007; 13: 1299–312.
  • Walter MN, Wright KT, Fuller HR, MacNeil S, Johnson WE. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. Exp Cell Res 2010; 316: 1271-81.

Investigation of the contribution of concentrated growth factor (CGF) and processed lipoaspirate (PLA) to wound healing in diabetic rats

Yıl 2021, Cilt: 4 Sayı: 1, 33 - 37, 21.01.2021
https://doi.org/10.32322/jhsm.804331

Öz

ABSTRACT
Aim: The aim of the study is to show the effectiveness of concentrated growth factor (CGF) and processed lipoaspirate (PLA) in wound healing in diabetic rats.
Materyal and Method: A total of 30 rats were used in the study. İt was divided into 3 groups as CGF, PLA and control group. The rats were made diabetic using Sreptozotocin IP. A 5mm diameter wound was created on one of the hind legs of all rats by using a punch. CGF and PLA were applied to the lesions. Daily wound size and wound condition were recorded on days 3, 5 and 10. At the end of the study, blood samples were taken for TNF-α, TGF-β, IL-1, PDGF, FGF and VEGF measurements before the rats were sacrificed.
Results: The mean wound diameters measured on the 3rd day in the study were 4.6 ± 0.06 mm in the control group, 4.1 ± 0.05 mm in the CGF group, and 4.4 ± 0.07 mm in the PLA group. The wound diameters measured on the 5th day were 3.1 ± 0.04 mm in the control group, 1.6 ± 0.05 mm in the CGF group and 2.7 ± 0.06 mm in the PLA group (P <0.01). The mean closure time of wounds was 5.3 ± 1.1 days in the CGF group, 7.1 ± 1.4 days in the PLA group, and 9.4 ± 0.5 days in the control group. All of the wounds were healed in all groups on the 10th day. This improvement rate in the CGF group was statistically significant compared to the other two groups (p<0.01). CGF and PLA increased the speed of wound healing in diabetic rats. Inflammatory marker levels (TNF-α, TGF-β, IL-1, PDGF, FGF, VEGF) obtained from blood samples were higher than normal in all rats and there was no significant difference between the groups (p>0.05).
Conclusion: In this study, it was shown that CGF application was more effective than PLA application in wound healing in diabetic rats.
Keywords: CGF, PLA, diabetic rat, ulcer

Proje Numarası

2016/005

Kaynakça

  • Harper D, Young A, McNaught CE. The physiology of wound healing. Surgery (Oxford) 2014; 32: 445-50.
  • Macdonald J, Asiedu K. WAWLC: World Alliance for Wound and Lymphedema Care. Wounds 2010; 22: 55-9.
  • Sayek İ, Ozmen MM, Temel Cerrahi El Kitabı, Guneş Tıp Kitabevleri, Ankara, 2009
  • Coşkun Ö, Uzun G, Dal D ve ark. Kronik yarada tedavi yaklaşımları. Gülhane Tıp Derg 2016; 58: 207-28.
  • Rajpaul K. Biofilm in wound care. Br J Community Nurs; Suppl Wound Care: 2015; 6: 10-1.
  • Broughton G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg 2006; 117: 125-34
  • Jeffcoate J.W, Price P, Harding G.K.Wound healing and treatments for people with diabetic foot ulsers. Dıabetes/Metabolısm Research and Reviews 2004; 20: 78–89.
  • Martino MM, Tortelli F, Mochizuki M, al. Engineering the growth factor microenvironment with fibronectin domains to promote wound and bone tissue healing. Sci Transl Med 2011; 3: 100-89.
  • Rodella LF, Favero G, Boninsegna R, et al. Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction. Microsc Res Tech 2011; 74: 772-7.
  • Chen Y, Cai Z, Zheng D, et al. Inlay osteotome sinus floor elevation with concentrated growth factor application and simultaneous short implant placement in severely atrophic maxilla. Scientific reports 2016; 6: 273-8.
  • Kim T-H, Kim S-H, Sándor GK, Kim Y-D. Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing. Archives of oral biology 2014; 59: 550-8.
  • İnan S, Ozbilgin K. Kok Hucre Biyolojisi. Sağlıkta Birikim. Cilt 1, Sayı 5, 2009: 11-23.
  • Kok IJ, Peter SJ, Archambault M et al. Investigation of allogeneic mesenchymal stem cell-based alveolar bone formation: preliminary findings. Clin Oral Implants Res 2003; 14: 481-9.
  • Öztopalan DF, Işık R, Durmuş AS. Yara iyileşmesinde büyüme faktörleri ve sitokinlerin rolü. Dicle Üniv Vet Fak Derg 2017; 10: 83-8
  • Greenhalgh DG. Wound healing and diabetes mellitus. Clin Plastic Surg 2003; 30: 37-45.
  • Galiano RD, Michaels VJ, Dobryansky M, Levine JP, Gurtner GC. Quantitative and reproducible murine model of excisional wound healing. Wound Repair Regen 2004; 12: 485-92.
  • Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabet Med 2006; 23: 594-608.
  • Larsen JA, Overstreet J. Pulsed radio frequency energy in the treatment of complex diabetic foot wounds: two cases. J Wound Ostomy Continence Nurs 2008; 35: 523-7.
  • Reid RR, Said HK, Mogford JE, Mustoe TA. The future of wound healing: Pursuing surgical models in transgenic and knockout mice. J Am Coll Surg 2004; 199: 578-85.
  • Guo SC, Tao SC, Yin WJ, Qi X, Yuan T, Zhang CQ. Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model. Theranostics 2017; 7: 81-96.
  • Yuan T, Guo SC, Han P, Zhang CQ, Zeng BF. Applications of leukocyte- and platelet-rich plasma (L-PRP) in trauma surgery. Curr Pharm Biotechnol 2012; 13: 1173–84.
  • Wang F, Sun Y, He D, Wang L. Effect of Concentrated Growth Factors on the Repair of the Goat Temporomandibular Joint. J Oral Maxillofac Surg 2017; 75: 498-507.
  • Shih B, Garside E, McGrouther DA, Bayat A. Molecular dissection of abnormal wound healing processes resulting in keloid disease. Wound Repair Regen 2010; 18: 139-53.
  • Ulaşlı AM. Kas iskelet sistemi yaralanmalarında plateletten zengin plazma tedavisi. Kocatepe Tıp Derg 2012: 13; 1-3.
  • Kim JM, Sohn DS, Bae MS, Moon JW, Lee JH, Park IS. Flapless transcrestal sinus augmentation using hydrodynamic piezoelectric internal sinus elevation with autologous concentrated growth factors alone. Implant Dent 2014; 23: 168-74.
  • Sohn D, Moon J, Moon Y, Park J, Jung H. The use of concentrated growth factors (CGF) for sinus augmentation. J Oral Implant 2009; 38: 25-38.
  • Khojasteh A, Eslaminejad MB, Nazarian H. Mesenchymal stem cells enhance bone regeneration in rat calvarial critical size defects more than platelete-rich plasma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106: 356-63.
  • Falanga V, Iwamoto S, Chartier M, et al. Autologous bone marrow-derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue Eng 2007; 13: 1299–312.
  • Walter MN, Wright KT, Fuller HR, MacNeil S, Johnson WE. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. Exp Cell Res 2010; 316: 1271-81.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Orijinal Makale
Yazarlar

Ali Bolat 0000-0002-2203-8419

Yıldırım Gültekin 0000-0002-9384-0556

Proje Numarası 2016/005
Yayımlanma Tarihi 21 Ocak 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 4 Sayı: 1

Kaynak Göster

AMA Bolat A, Gültekin Y. Investigation of the contribution of concentrated growth factor (CGF) and processed lipoaspirate (PLA) to wound healing in diabetic rats. J Health Sci Med / JHSM. Ocak 2021;4(1):33-37. doi:10.32322/jhsm.804331

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Assoc. Prof. Alpaslan TANOĞLU (MD)  

Prof. Aydın ÇİFCİ (MD)

Prof. İbrahim Celalaettin HAZNEDAROĞLU (MD)

Prof. Murat KEKİLLİ (MD)

Prof. Yavuz BEYAZIT (MD) 

Prof. Ekrem ÜNAL (MD)

Prof. Ahmet EKEN (MD)

Assoc. Prof. Ercan YUVANÇ (MD)

Assoc. Prof. Bekir UÇAN (MD) 

Assoc. Prof. Mehmet Sinan DAL (MD)


Our journal has been indexed in DOAJ as of May 18, 2020.

Our journal has been indexed in TR-Dizin as of March 12, 2021.


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Articles published in the Journal of Health Sciences and Medicine have open access and are licensed under the Creative Commons CC BY-NC-ND 4.0 International License.