Overview of the angiogenic effect of probiotics (Lactobacillus acidophilus and Lactobacillus rhamnosus) at human umbilical vein endothelial cells

Yıl 2022, Cilt: 5 Sayı: 3, 765 - 770, 30.05.2022

Mualla Pınar Elçi Tuğba Fatsa Sinem Kaya Nesli Ersoy Merve Alpay Taner Özgürtaş

https://doi.org/10.32322/jhsm.1025896

Öz

Introduction: Angiogenesis (neovascularization), which means new vessel construction, is normal and physiologically, wound healing, embryogenesis, a necessary menstrual cycle it's a mechanism. When taken in appropriate amounts together with or separately with nutrients, mucosal and by regulating systemic immunity, ensuring nutritional and microbial balance in the intestines living nonpatogenic microorganisms that positively affect the health of the host it is called "probiotics". Lactic acid bacteria, the most probiotic microorganisms it constitutes its important group. Where probiotics have an effect on angiogenesis, and it is thought to help heal wounds through the road. With this research indicated that roles of Lactobacillus acidophilus and Lactobacillus rhamnosus on angiogenesis if present to demonstrate in vitro methods and the gene expression responsible for the formation of these effects it is intended to reveal.
Material and Method: This study is an experimental study conducted in vitro human umbilical cord vein endothelial cell (HUVEC) MTT test in cell culture with (3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide; Thiazolyl blue) evaluation of viability and proliferation wound healing model, tube formation method and gene expression with real rime-polymer chain reaction (RT-PCR) methods of appointment were used.
Results: HUVEC cells L. acidophilus 10⁹ CFU\ml after extract application statistical of mRNA expression of VEGF and FGF genes by control group 24 per hour it was found to increase significantly. L. rhamnosus 10 6 CFU\ml and 109 CFU\ml after application of extracts VEGF gene mRNA by control group 24 per hour its expression was found to be statistically significantly increased. Also L. rhamnosus extracts cell proliferation and migration of in vitro wound model it was found to increase statistically significantly.
Conclusion: In this study, in vitro L. acidophilus 10⁹ CFU\ml extract and 10⁶ CFU\ml and 10⁹ CFU\ml extract of L. rhamnosus, VEGF gene mRNA revealed to be effective on angiogenesis in HUVEC cells by increasing expression it is.

Anahtar Kelimeler

Probiotics, angiogenesis, HUVEC, wound healing model

Kaynakça

• Nie D, Tang K, Diglio C, Honn KV. Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase. Blood 2000; 95: 2304-11.
• Dharmani P, Simone CD, Chadee K. The probiotic mixture VSL# 3 accelerates gastric ulcer healing by stimulating vascular endothelial growth factor. Plos One 2013; 8: 58671.
• Ersoy N, Açıkgöz A, Özgürtaş T. Probiyotikler ve yara iyileşme sürecine etkileri: anjiyogenez üzerine etki mekanizması. J Nutr Diet 2018; 46: 303-10.
• El-Ghazely M, Mahmoud WH, Atia MA, Eldip EM. Effect of probiotic administration in the therapy of pediatric thermal burn. Ann Burns Fire Disasters 2016; 29: 268.
• Ashoori Y, Mohkam M, Heidari R. Development and in vivo characterization of probiotic lysate-treated chitosan nanogel as a novel biocompatible formulation for wound healing. Biomed Res Int 2020: 8868618.
• Kerry RG, Patra RJ, Gouda S, Park Y, Shin H, Das G. Benefaction of probiotics for human health: A review. J Food Drug Anal 2018; 26: 927-39.
• Tsiouris CG, Kelesi M, Vasilopoulos G, Kalemikerakis J, Papageorgiou EG. The efficacy of probiotics as pharmacological treatment of cutaneous wounds: meta-analysis of animal studies. Eur J Pharm Sci 2017. 104: 230-9.
• Tsiouris CG, Tsiouri MG. Human microflora, probiotics and wound healing. Wound Medicine 2017. 19: 33-38.
• Fuller R. Probiotic in man and animals. J Appl Bacteriol 1989; 66: 131-9.
• Oh S, Kim SH, Worobo RW. Characterization and purification of a bacteriocin produced by a potential probiotic culture, Lactobacillus acidophilus 30SC. J Dairy Sci 2000; 83: 2747-52.
• Gürsoy O, Kınık Ö, Gönen İ. Probiyotikler ve gastrointestinal sağlığa etkileri. Türk Biyoloji Cemiyeti Derg 2005: 136-48.
• Parvez S, Malik KA, Kang SA, Kim HY. Probiotics and their fermented food products are beneficial for health. J Appl Microbiol 2006; 100: 1171-85.
• Vinderola CG, Prosello W, Ghiberto TD, Reinheimer JA. Viability of probiotic (Bifidobacterium, Lactobacillus acidophilus and Lactobacillus casei) and nonprobiotic microflora in Argentinian Fresco cheese. J Dairy Sci 2000; 83: 1905-11.
• Hotel AC, Cordoba AJ, et al. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Prevention 2001; 5: 1-10.
• Saarela M, Mogensen G, Fondén R, Mättö J, Sandholm T. Probiotic bacteria: safety, functional and technological properties. J Biotechnol 2000; 84: 197-215.
• Reid G, Burton J. Use of Lactobacillus to prevent infection by pathogenic bacteria. Microbes Infect 2002; 4: 319-24.
• Gopal PK., Sullivan P, Smart J. Utilisation of galacto-oligosaccharides as selective substrates for growth by lactic acid bacteria including Bifidobacterium lactis DR10 and Lactobacillus rhamnosus DR20. International Dairy Journal 2001; 11: 19-25.
• Goldin BR, Gorbach SL, Saxelin M, Barakat S, Gualtieri L, Salminen S. Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Dig Dis Sci 1992; 37: 121-8.
• 19. Teuber M, Schwarz F, Meile L. Acquired antibiotic resistance in lactic acid bacteria from food. Antonie Van Leeuwenhoek 1999; 76: 115-37.
• Mathur S, Singh R. Antibiotic resistance in food lactic acid bacteria—a review. Int J Food Microbiol 2005; 105: 281-95.
• D'Aimmo MR, Modesto M, Biavati B. Antibiotic resistance of lactic acid bacteria and Bifidobacterium spp. isolated from dairy and pharmaceutical products. Int J Food Microbiol 2007; 115: 35-42.
• Avonts L, Uytven EV, De Vuyst L. Cell growth and bacteriocin production of probiotic Lactobacillus strains in different media. International Dairy Journal 2004; 14: 947-55.
• González F, Guerrero C, Quijano G. The benefits of probiotics on human health. J Microbial Biochem Technol 2011; 1: 1948-5948
• Qinghui Mu, Vincent J, Luo X. Role of Lactobacillus reuteri in Human Health and Diseases. Front. Microbiol 2018.
• Halper J, Leshin LS, Lewis SJ, Li W. Wound healing and angiogenic properties of supernatants from Lactobacillus cultures. Exp Biol Med 2003; 228: 1329-37.
• Elliott SN, Buret A, McKnight W, Miller M, John L. Wallace Bacteria rapidly colonize and modulate healing of gastric ulcers in rats. Am J Physiol 1998; 275: 425-32.
• Lombardi F, Palumbo P, Mattei A, et al. Soluble fraction from lysates of selected probiotic strains differently influences re-epithelialization of HaCaT scratched monolayer through a mechanism involving nitric oxide synthase 2. Biomolecules 2019; 9: 756.
• Sinha A, Sagar S, Madhumathy M, Osborne W. Probiotic bacteria in wound healing; an in-vivo study. Iranian J Biotech 2019; 17: 2188.
• Singh PK, Kaur P. Synbiotic (probiotic and ginger extract) loaded floating beads: a novel therapeutic option in an experimental paradigm of gastric ulcer. J Pharm Pharmacol 2012; 64: 207-17.
• Gudadappanavar AM, Hombal PR, Timashetti SS, Javali SB. Influence of Lactobacillus acidophilus and Lactobacillus plantarum on wound healing in male Wistar rats-an experimental study. Int J App Basic Med Res 2017; 7: 233.
• Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic M. Growth factors and cytokines in wound healing. Wound Repair Regen 2008; 16: 585-601.
• Zahedi F, Nasrabadi M, Tajabadi M, Aboutalebi H. Comparison of the effects of Lactobacillus brevis and Lactobacillus plantarum on cutaneous wound healing in rats. African J Microbiol Res 2011; 5: 4226-33.
• Giorgetti G, Brandimarte G, Fabiocchi F. Interactions between innate immunity, microbiota, and probiotics. J Immunol Res 2015: 501361.