Evaluation and epigenetic impact of B12, vitamin D, folic acid and anemia in Hashimoto's thyroiditis: a clinical and molecular docking study

Year 2023, Volume: 6 Issue: 4, 705 - 712, 30.07.2023

Elif Sibel Aslan , Savaş Gür

https://doi.org/10.32322/jhsm.1243597

Cited By: 1

Abstract

Aims: Our approach in this study is to investigate the collective effect of various parameters, including vitamin B12 (B12), vitamin D (Vit-D), folic acid, and iron deficiency, on Hashimoto's thyroiditis (HT) disease. This differs from existing literature that has examined these parameters individually.
Methods: The study evaluated age, gender, thyroid stimulating hormone (TSH), free-T4 (FT4), free-T3 (FT3), Vit-D levels, as well as autoantibodies against thyroid peroxidase (anti-TPO) and anti-tyroglobulin iron (anti-TG) levels, ferritin, B12, and folic acid parameters in a total of 30 HT patients and 37 non-HT patients. These parameters were assessed by analyzing the patients' routine blood test results using automated analysis methods.
Results: A negative correlation was found between the blood, Vit-D level and anti-TG (as the vit-D value increases, the anti-TG decreases) (r=-0.417; p=0.001; p<0.01). There was a statistically significant and weak correlation between blood Vit-D measurements and anti-TPO measurements (r=-0.341; p=0.005; p<0.01).
Conclusion: The study findings demonstrated that there was no statistically significant difference in the measurements and correlation between hemoglobin, FT3, FT4, TSH, ferritin, vitamin B12, and folic acid levels in patients with and without a diagnosis of HT (p>0.05). However, the study emphasized the critical role of vitamin D in the pathophysiology and treatment of HT. Furthermore, molecular docking simulations indicated that folic acid could potentially act as a potent inhibitor of human extracellular signal-regulated kinase (ERK2), which has been reported to play a key role in HT.

Keywords

Hashimoto, otoimmun disease, vitamin D, anti-TPO, ferritin, vitamin B12

Thanks

I can acknowledge the all authors. The support of Dr. Abdulilah Ece is gratefully acknowledged in computational part.

Evaluation and epigenetic impact of B12, vitamin D, folic acid and anemia in Hashimato's thyroiditis

Abstract

Aim:This study aimed to evaluate the frequency of B12, vitamin D, folic acid and iron deficiency, which play a role in the pathogenesis and epigenetic effect on HT, compared to individuals without thyroid disease. Materials and Methods:Age, gender, thyroid stimulating hormone (TSH), free-T4 (FT4), free-T3 (FT3), vit-D among 30 HT patients and 37 non-HT patients admitted to the outpatient clinic between January and December 2021, anti-TPO and Anti-Tyroglobulin iron, vit-D, ferritin, B12, folic acid parameters were evaluated by checking the patients routine blood test results taken by autoanalysis between groups. Results:When the vit-D levels of 30 patients with HT and 37 patients without a diagnosis of HT were compared, a difference was found between vit-D levels. A negative correlation was found between the blood, vit-D level and Anti-TG (as the vit-D value increases, the Anti-Tyroglobulin value decreases) (r=-0.417; p=0.001; p<0.01). There was a statistically significant and weak correlation between blood vit-D measurements and Anti-TPO measurements (r=-0.341; p=0.005; p<0.01). Conclusion:When evaluated in terms of Hemoglobin, FT3, FT4, TSH, Ferritin, Vitamin B12, and Folic acid between patients with and without a diagnosis of HT, their measurements did not show a statistically significant difference (p>0.05). Once again, the importance of vit-D deficiency in the physiopathology and treatment of HT, the elimination of vit-D deficiency have actually been shown how important in the treatment process of HT. On the other hand, molecular docking simulations revealed that folic acid might be a strong inhibitor of human extracellular signal-regulated kinase (ERK2) which was reported to have a key role in HT.

Keywords

Hashimoto, otoimmun disease, Vitamin D (vit-D), anti-TPO, Ferritin, Vitamin B12

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