Yağda çözünen postbiyotiklerle yeni mikrobiyom dostu temizleyici yağ formülasyonu

Bu makalenin ilk hali 14 Şubat 2025 tarihinde yayımlandı. https://dergipark.org.tr/tr/pub/jicm/article/1580770

Düzeltme Notu

Yazar sıralamasının yazarların talebi doğrultusunda doğru düzenlenmesi ve bir teşekkür mektubu eklenmesi nedeniyle düzeltme metni oluşturulmuştur. TEŞEKKÜR Bu araştırma projesinin gerçekleştirilmesi Teknopark Geliştirme Bölgesi-Üniversite ve Sanayi işbirliği ile mümkün olmuştur. Yazarlar, The Purest Solutions Purifying Oil Cleanser with Postbiotics üreticisi EVLY Pharma Cosmetics Inc. ve TPS Academy'ye destekleri için teşekkürlerini sunar.

Öz

Amaç: Son yıllarda hastalıklardan korunmada beslenmenin etkinliğinin yanında koruyucu kozmetik bakım da giderek önem kazanmıştır. Koruyucu yaklaşımda özellikle mikrobiyotanın etkinliği pek çok çalışmada yer almıştır. Mikrobiyotanın durumu, vücuttaki enflamasyon, çeşitli metabolik hastalıklar, dermal hastalıklar, nörodejeneratif hastalıklar veya dermal hastalıklarla ilişkilendirilmiştir. Bu çalışmada, yağ içinde çözündürülmüş postbiyotik hammadde kullanılarak yapılan temizleyicinin cilt mikrobiyotasına ve hücre üzerine olumlu etkilerinin araştırılması amaçlanmıştır. Bu çalışmada, %2 postbiyotik LTO 35 içeren cilt temizleyici yağın, normal keratinositler (HEKA 500K CELL) ve cilt mikrobiyota simülasyonu (Lactobacillus crispatus LTC-KC24011, Staphylococcus epidermidis ATA-LSE 0198052, Staphylococcus aureus ATA-LTCA 011204, Staphylococcus capitis ATA -LSC 0201201, Propionibacterium acnes ATA-LPC 0204221, Streptococcus pyogenes ATA TCSP 210911, Candida albicans ATA-LTCA 0504212) üzerindeki etkileri değerlendirilmiştir. Sonuçlar değerlendirildiğinde, keratinositlere zarar vermediği, mikrobiyota dengesini koruduğu ve hücresel onarıma destek olduğu görülmüştür. Bu çalışmada, yağ içinde çözündürülmüş postbiyotik hammadde kullanılarak yapılan temizleyicinin cilt mikrobiyotasına ve hücre üzerine olumlu etkilerinin araştırılması amaçlanmıştır. Yöntem: Bu çalışmada, %2 postbiyotik LTO 35 içeren cilt temizleyici yağın, normal keratinositler (HEKA 500K CELL) ve cilt mikrobiyota simülasyonu (Lactobacillus crispatus LTC-KC24011, Staphylococcus epidermidis ATA-LSE 0198052, Staphylococcus aureus ATA-LTCA 011204, Staphylococcus capitis ATA -LSC 0201201, Propionibacterium acnes ATA-LPC 0204221, Streptococcus pyogenes ATA TCSP 210911, Candida albicans ATA-LTCA 0504212) kullanılmıştır. Simülasyonda kullanılan bakteriler ve formülasyon içinde kullanılan postbiyotik (LTO 35 Streptococcus thermophilus ATA-LTC St140700, Bifidobacterium animalis ATA-BSLA0310, Lactobacillus acidophilus ATA-LAP1201 ferment ekstrakt in oil lizat) ATA BIO Teknoloji kültür koleksiyonundan temin edilmiştir. Simülasyon kontak süresi 1 saat olarak uygulanmıştır. Bulgular: Bu çalışmada kullanılan Purifying Oil Cleanser with Postbiotics numunesi, mikrobiyolojik açıdan uygun olarak değerlendirilmiştir. Denge testi olumlu bulunup, patojen yönünde değişmediği gözlenmiştir. Yapılan analizlere göre; Purifying Oil Cleanser with Postbiotics numunesinin cilt mikrobiyotası çeşitliliğine zarar vermediği ve mikrobiyom dostu olduğu ifade edilebilir. Sonuç: Sonuçlar değerlendirildiğinde, keratinositlere zarar vermediği, mikrobiyota dengesini koruduğu ve hücresel onarıma destek olduğu görülmüştür.

Anahtar Kelimeler

• Probiyotik
• Postbiyotik
• Mikrobiyota
• Yağ Temizleyici
• Kozmetik

Novel microbiome friendly purifying oil cleanser formulation with oil-soluble postbiotics

Bu makalenin ilk hali 14 Şubat 2025 tarihinde yayımlandı. https://dergipark.org.tr/tr/pub/jicm/article/1580770

Düzeltme Notu

Corrigendum was created due to the correct arrangement of the author order in line with the request of the authors and the addition of a thank you letter. ACKNOWLEDGEMENT The realization of this research project was made possible by the cooperation of Technopark Development Zone-University and Industry. The authors would like to express their gratitude to The Purest Solutions Purifying Oil Cleanser with Postbiotics manufacturer EVLY Pharma Cosmetics Inc. and TPS Academy for their support.

Öz

Objective: In recent years, the field of preventive cosmetic care has gained importance alongside the established effectiveness of nutrition in preventing diseases. Within the scope of this preventive approach, the microbiota has been a focal point in numerous studies, with its state being associated with a range of health concerns, including inflammation, metabolic diseases, dermatological diseases, and neurodegenerative diseases.The present study aims to investigate the positive effects of a cleanser formulated with a postbiotic raw material dissolved in oil on skin microbiota and cells. In this study, the skin cleansing oil containing 2% postbiotic LTO 35 was utilised as a skin microbiota simulation (Lactobacillus crispatus LTC-KC24011, Staphylococcus epidermidis ATA-LSE 0198052) and normal keratinocytes (HEKA 500K CELL), Staphylococcus aureus ATA-LTCA 011204, Staphylococcus capitis ATA-LSC 0201201, Propionibacterium acnes ATA-LPC 0204221, Streptococcus pyogenes ATA-TCSP 210911, and Candida albicans ATA-LTCA 0504212. The results demonstrated that the cleanser did not cause harm to keratinocytes, maintained microbiota balance, and promoted cellular repair.The objective of this study was to investigate the positive effects of a cleanser made using oil-solubilised postbiotic raw material on skin microbiota and cells. Material&Method: In this study, a skin cleansing oil containing 2% postbiotic LTO 35 was utilised to simulate normal keratinocytes (HEKA 500K CELL) and skin microbiota (Lactobacillus crispatus LTC-KC24011, Staphylococcus epidermidis ATA-LSE 0198052, Staphylococcus aureus ATA -LTCA 011204, Staphylococcus capitis ATA-LSC 0201201, Propionibacterium acnes ATA-LPC 0204221, Streptococcus pyogenes ATA TCSP 210911, Candida albicans ATA-LTCA 0504212. The bacteria utilised in the simulation and the postbiotic employed in the formulation (LTO 35 Streptococcus thermophilus ATA-LTC St140700, Bifidobacterium animalis ATA-BSLA0310, Lactobacillus acidophilus ATA-LAP1201 ferment extract in oil lysate) were obtained from the ATA BIO Technology culture collection. The simulation contact time was applied as 1 hour. Results: The Purifying Oil Cleanser with Postbiotics sample examined in this study was found to be microbiologically suitable. The equilibrium test yielded a positive result, and it was observed that the sample did not change in the direction of pathogens. According to the analyses, it can be concluded that the Purifying Oil Cleanser with Postbiotics sample does not harm the diversity of skin microbiota and is microbiome-friendly. Conclusion: Upon evaluation of the results, it was observed that the substance did not cause harm to keratinocytes, it maintained microbiota balance, and it promoted cellular repair.

Anahtar Kelimeler

• Probiotic
• Postbiotic
• Microbiota
• Oil Cleanser
• Cosmetics

Kaynakça

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