Enkapsüle edilmiş kenevir tohumu yağının antimikrobiyal aktivite çalışmaları

Öz

Giriş: Bu çalışmada Cannabis sativa bitkisinin tohumlarından elde edilen yağın stabilizasyon, emilim ve dağılım etkisini arttıracak lipozom ve lipozom- jel formülasyonlarının antimikrobiyal etkinliğini ne ölçüde etkilediğini değerlendirmek amaçlanmıştır. Gereç ve Yöntem: Çalışmamda antimiktobiyal etken olarak Kenevir Tohumu Yağı kullanıldı. Mikroorganizma olarak Candida albicans araştırıldı. Kenevir tohumu yağından 8 farklı formülasyon hazırlanarak yayma plak yöntemiyle etki incelendi. Bulgular: Kenevir Tohumu Yağının formülasyon uygulamaları ve kontrol gruplarına göre en etkili sonuçlar, Carbopol® Ultrez 30 içeren lipozomal formülasyonda gözlendi. C. albicans ile yapılan yayma ekim çalışmasında, Kenevir Tohumu Yağını formülasyonları ve kontrol gruplarının bir hafta süresince yapılan incelemelerde çoğalmasının sınırlandığı belirlenmiştir. Sonuç: Hazırlanan Kenevir Tohumu Yağı formülasyonları, çalışmada kullanılan C. albicans’ a çoğalmasını büyük oranda önleyici etkisi olduğu tespit edildi. Ulaşılan bu sonuçlara bakıldığı zaman, Kenevir Tohmu Yağının ve C. albicans’ ın faydalanıldığı çalışmalara zemin hazırlayacaktır.

Anahtar Kelimeler

• Antimikrobiyal Aktivite
• Kenevir Tohumu Yağı
• Candida albicans
• Lipozom
• Lipozom- jel

Antimicrobial activity studies of encapsulated hemp seed oil

Öz

Objective: In this study, it was aimed to evaluate to what extent the oil obtained from the seeds of the C. sativa plant affects the antimicrobial activity of liposome and liposome-gel formulations that will increase the stabilization, absorption and dispersion effect. Materials and Method: Hemp Seed Oil was used as an antimicrobial agent in our study. C. albicans was investigated as a microorganism. Eight different formulations were prepared from cannabis seed oil and the effect was examined by the spread plate method. Results: The most effective results were observed in the liposomal formulation containing Carbopol® Ultrez 30 compared to the formulation applications and control groups of Cannabis Seed Oil. In a smear sowing study with C. albicans, it was determined that the growth of Cannabis Seed Oil formulations and control groups was limited during one-week examinations. Conlusion: It was determined that the prepared Cannabis Seed Oil formulations had a highly inhibitory effect on the proliferation of C. albicans used in the study. Considering these results, it will lay the groundwork for studies that benefit from Cannabis Seed Oil and C. albicans.

Anahtar Kelimeler

• Antimicrobial activity
• Candida albicans
• Hemp seed oil
• Liposome
• Liposome-gel

Kaynakça

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