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Mixed Onychomycosis Case with Fusarium Solani

Yıl 2022, Cilt: 7 Sayı: 2, 45 - 50, 30.06.2022

Öz

Background: Fusarium species are found in soil and are important plant pathogens. Fusarium species may cause nail and corneal infections in healthy people.
Materials and Methods: A 20-year-old male patient presented to Trakya University Hospital with complaints of discoloration and dullness of the nail that had started 2-3 months prior. A Pseudomonas infection was considered, as a greenish colour change was observed in the nail of the first digit on the left hand. As the greenish colour on the nail began to turn black, a fungal examination was performed. Total nail avulsion was planned when hyphae were observed in a direct preparation of the nail scraping sample with 30% KOH. In the first scraping sample after nail removal, fungal hyphae were observed in a direct preparation with 30% KOH. Culturing process, bacteria, yeast, saprophytic fungi were observed. The cultures were repeated after nail removal, and the results were similar to the previous cultures.
Conclusions: Fusarium solani, Candida parapsilosis and Pseudomonas aeruginosa grew in both cultures. Fusarium solani was identified by using molecular methods in addition to its macroscopic and microscopic features. Sequence analysis was performed with Sanger sequencing. Species identification was confirmed via phylogenetic data analysis based on DNA of the obtained sequences. Candida parapsilosis and Pseudomonas aeruginosa were identified with the classical method and VITEK® 2 Compact.

Kaynakça

  • Referans1 Coleman JJ. Fusarium solani species complex: ubiquitous pathogens of agricultural importance. Mol Plant Pathol 2016; 17(2): 146-158.
  • Referans2 Larone DH (Edited by). Medically Important Fungi. 4th Edition, ASM Press, 1752 N St., N.W., Washington, 20036-2904, USA, 2002:280.
  • Referans3 Uslu H, Aktas AE, Celebi D, et al. The foot fungal flora of medical faculty students. Eurasian J Med 2004; 36: 53-56.
  • Referans4 Ghannoum M,Isham N. Fungal nail infections (onychomycosis): a never-ending story? PLoS Pathog 2014; 10(6):e1004105.
  • Referans5 Mugge C, Haustein UF, Nenoff P. Causative agents of onychomycosis-a retrospective study. J Dtsch Dermatol Ges 2006: 4(3); 218-228.
  • Referans6 Baran R, Tosti A, Piraccini BM. Uncommon clinical patterns of Fusarium nail infection: report of three cases. Br J Dermatol 1997: 136(3); 424-427.
  • Referans7 Mermel LA, McKay M, Dempsey J, et al. Pseudomonas surgical-site infections linked to a healthcare worker with onychomycosis. Infect Control Hosp Epidemiol 2003; 24: 749-752.
  • Referans8 Yang YS, Ahn JJ, Shin MK, et al. Fusarium solani onychomycosis of the thumbnail coinfected with Pseudomonas aeruginosa: report of two cases. Mycoses 2009; 54(2): 168-171.
  • Referans9 Tatu AL, Voicu C, Clatici VG. Therapeutic management of a Green Nail Syndrome-Pseudomonas aeruginosa and Trichophyton tonsurans coinfection detected by mass spectrometry conduita terapeutica a sindromului unghiilor verzi-coinfectia cu Pseudomonas aeroginosa şı Trichophyton tonsurans Romanian Journal of Clinical and Experimental Dermatology 2016; 3(3-4): 167-169.
  • Referans10 Hogan DA, Kolter R. Pseudomonas-Candida interactions: an ecological role for virulence factors. Science 2002; 296(5576): 2229-2232.

Fusarium Solani’ye Bağlı Miks Onikomikoz Olgusu

Yıl 2022, Cilt: 7 Sayı: 2, 45 - 50, 30.06.2022

Öz

Amaç: Fusarium türleri toprakta bulunur ve önemli bitki patojenleridir. Fusarium türleri sağlıklı kişilerde tırnak ve kornea enfeksiyonlarına neden olabilir.
Gereç ve Yöntem: 20 yaşındaki erkek hasta, 2-3 ay önce başlayan tırnakta renk değişikliği ve matlaşma şikâyetleriyle Trakya Üniversitesi Tıp Fakültesi Hastanesi’ne başvurdu. Sol el birinci tırnağında yeşilimsi renk değişikliği saptanan olguya Pseudomonas’a bağlı enfeksiyon düşünüldü. Tırnaktaki yeşilimsi renk siyahlaşmaya başladığından fungal inceleme yapıldı. Tırnak kazıntı örneğinin %30’luk KOH’lu direkt preparatında hifler görülmesi üzerine tırnak çekimi planlandı. Hastanın tırnak çekimi gerçekleşmesinden sonraki alınan ilk kazıntı örneğine %30’luk KOH’lu direkt preparatında mantar hifleri görüldü. Kültürde bakteri, maya mantarı, saprofit fungus üremesi oldu. Tırnak çekimi yapıldıktan sonra kültürler tekrarlandı. Bir önceki ekimi yapılan kültür ile sonuç benzerdi.
Sonuç: Her iki kültür de Fusarium solani, Candida parapsilosis ve Pseudomonas auroginosa üredi. Fusarium solani makroskobik ve mikroskobik özellikleri ile birlikte moleküler yöntemler kullanılarak isimlendirildi. Sangers Sekansı yöntemi ile dizi analizi yapıldı. Elde edilen sekans dizileri ile DNA tabanında flogenetik veri analizi gerçekleştirilerek tür tayini doğrulandı. Klasik yöntem ve VİTEK 2 compact ile Candida parapsilosis ve Pseudomonas auroginosa’nın identifikasyonu yapıldı.

Kaynakça

  • Referans1 Coleman JJ. Fusarium solani species complex: ubiquitous pathogens of agricultural importance. Mol Plant Pathol 2016; 17(2): 146-158.
  • Referans2 Larone DH (Edited by). Medically Important Fungi. 4th Edition, ASM Press, 1752 N St., N.W., Washington, 20036-2904, USA, 2002:280.
  • Referans3 Uslu H, Aktas AE, Celebi D, et al. The foot fungal flora of medical faculty students. Eurasian J Med 2004; 36: 53-56.
  • Referans4 Ghannoum M,Isham N. Fungal nail infections (onychomycosis): a never-ending story? PLoS Pathog 2014; 10(6):e1004105.
  • Referans5 Mugge C, Haustein UF, Nenoff P. Causative agents of onychomycosis-a retrospective study. J Dtsch Dermatol Ges 2006: 4(3); 218-228.
  • Referans6 Baran R, Tosti A, Piraccini BM. Uncommon clinical patterns of Fusarium nail infection: report of three cases. Br J Dermatol 1997: 136(3); 424-427.
  • Referans7 Mermel LA, McKay M, Dempsey J, et al. Pseudomonas surgical-site infections linked to a healthcare worker with onychomycosis. Infect Control Hosp Epidemiol 2003; 24: 749-752.
  • Referans8 Yang YS, Ahn JJ, Shin MK, et al. Fusarium solani onychomycosis of the thumbnail coinfected with Pseudomonas aeruginosa: report of two cases. Mycoses 2009; 54(2): 168-171.
  • Referans9 Tatu AL, Voicu C, Clatici VG. Therapeutic management of a Green Nail Syndrome-Pseudomonas aeruginosa and Trichophyton tonsurans coinfection detected by mass spectrometry conduita terapeutica a sindromului unghiilor verzi-coinfectia cu Pseudomonas aeroginosa şı Trichophyton tonsurans Romanian Journal of Clinical and Experimental Dermatology 2016; 3(3-4): 167-169.
  • Referans10 Hogan DA, Kolter R. Pseudomonas-Candida interactions: an ecological role for virulence factors. Science 2002; 296(5576): 2229-2232.
Toplam 10 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Vaka Raporları
Yazarlar

Melek Tikveşli

Saban Gurcan

Erkut Afyoncu

Yıldız Gürsel Ürün

Sezgi Sarıkaya Solak

Yayımlanma Tarihi 30 Haziran 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 7 Sayı: 2

Kaynak Göster

APA Tikveşli, M., Gurcan, S., Afyoncu, E., Gürsel Ürün, Y., vd. (2022). Mixed Onychomycosis Case with Fusarium Solani. Journal of Immunology and Clinical Microbiology, 7(2), 45-50.
AMA Tikveşli M, Gurcan S, Afyoncu E, Gürsel Ürün Y, Sarıkaya Solak S. Mixed Onychomycosis Case with Fusarium Solani. J Immunol Clin Microbiol. Haziran 2022;7(2):45-50.
Chicago Tikveşli, Melek, Saban Gurcan, Erkut Afyoncu, Yıldız Gürsel Ürün, ve Sezgi Sarıkaya Solak. “Mixed Onychomycosis Case With Fusarium Solani”. Journal of Immunology and Clinical Microbiology 7, sy. 2 (Haziran 2022): 45-50.
EndNote Tikveşli M, Gurcan S, Afyoncu E, Gürsel Ürün Y, Sarıkaya Solak S (01 Haziran 2022) Mixed Onychomycosis Case with Fusarium Solani. Journal of Immunology and Clinical Microbiology 7 2 45–50.
IEEE M. Tikveşli, S. Gurcan, E. Afyoncu, Y. Gürsel Ürün, ve S. Sarıkaya Solak, “Mixed Onychomycosis Case with Fusarium Solani”, J Immunol Clin Microbiol, c. 7, sy. 2, ss. 45–50, 2022.
ISNAD Tikveşli, Melek vd. “Mixed Onychomycosis Case With Fusarium Solani”. Journal of Immunology and Clinical Microbiology 7/2 (Haziran 2022), 45-50.
JAMA Tikveşli M, Gurcan S, Afyoncu E, Gürsel Ürün Y, Sarıkaya Solak S. Mixed Onychomycosis Case with Fusarium Solani. J Immunol Clin Microbiol. 2022;7:45–50.
MLA Tikveşli, Melek vd. “Mixed Onychomycosis Case With Fusarium Solani”. Journal of Immunology and Clinical Microbiology, c. 7, sy. 2, 2022, ss. 45-50.
Vancouver Tikveşli M, Gurcan S, Afyoncu E, Gürsel Ürün Y, Sarıkaya Solak S. Mixed Onychomycosis Case with Fusarium Solani. J Immunol Clin Microbiol. 2022;7(2):45-50.

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