Swelling and re-swelling performance of glutaraldehyde crosslinked wet chitosan hydrogels

Öz

Chitosan (Chi) is frequently used in hydrogels because of its natural characteristics. It is environmentally friendly, biodegradable, and does not include any harmful substances. Chitosan hydrogels were chemically synthesized by cross-linking with glutaraldehyde (GA) at various ratios ranging from 1% to 10% (w/w), employing two types of molecular weight chitosan. The degree of swelling of wet hydrogels (non-drying) was examined at pH 2.0, 5.6, and 7.4. After the swollen hydrogels dried, their re-swelling capabilities were examined in distilled water. Chemically cross-linked chitosan hydrogels were found to be stable, so the hydrogels were able to swell repeatedly 5 times without losing any of their swelling properties. The storage modulus properties of chitosan hydrogels were measured using a Discovery Hybrid Rheometer-1 at different temperatures. The storage moduli of chitosan hydrogels remained stable as temperature increased, with maximum values of 0.01 MPa and 0.02 MPa for low and medium molecular weight hydrogels. The viscosity of chitosan solutions was determined using two instruments: a Discovery Hybrid Rheometer-1 at 25 °C at different shear rates, and a Brookfield Viscometer. The viscosity of low and medium molecular weight chitosan solutions were measured using a Brookfield Viscometer, resulting in values of 101.5 and 333.5 cps, respectively.

Anahtar Kelimeler

• Chitosan
• Glutaraldeyhe
• Re-swelling
• Wet hydrogel

Proje Numarası

Thesis Project (2020/YL/0025).

Kaynakça

1. Nangia S, Katyal DN, and Warkar SG (2021) Kinetics, absorption and diffusion mechanism of crosslinked Chitosan Kinetics, absorption and diffusion mechanism of crosslinked Chitosan Hydrogels. Ind J Eng Mater Sci 28:374–384.
2. Karagöz İ, Yücel G (2020) Use of super absorbent polymers with Euonymus plants (Euonymus japonicus ‘Aureomarginatus’) in ornamental plant cultivation. Journal of Agricultural Sciences 26(2):201-211.
3. Wei B, Zou J, Pu Q, Shi K, Xu B, and Ma Y (2022) One-step preparation of hydrogel based on different molecular weights of chitosan with citric acid. J. Sci of Food and Agri 102(9):3826–3834.
4. Gupta KC, Jabrail FH (2008) Effect of molecular weight and degree of deacetylation on controlled release of isoniazid from chitosan microspheres. Poly Adv Tech 19:432–441.
5. Szymańska E, Winnicka K (2015) Stability of chitosan - A challenge for pharmaceutical and biomedical applications. Marine Drugs. 13(4):1819–1846.
6. Celebi M, Ozdemir ZO (2017) Dyestuffs removal from synthetic wastewater with chitosan, cross-linked chitosan and chitosan-poly (acrylic acid) conjugate. Tekstil ve Konfeksiyon 27(3):283–288.
7. Hee DH, Da EN, Dong HS et al (2004) Preparation and biodegradation of thermosensitive chitosan hydrogel as a function of pH and temperature. Macr Res 12(5):507–511.
8. Üçel İS, Demirel E (2022) Modification of PVDF Membranes Using Dopamine/Zinc Oxide for Lead Removal from Aqueous Media. Open Journal of Nano 7(2):53-73.

9. Kaçoğlu HS, Ceylan Ö, Çelebi M (2024) Comparative study of the effect of cross-linking degree on chitosan hydrogels synthesized with low and medium molecular weight chitosan. Poly Eng and Sci 64(3):1326-1339.
10. Liu L, Gao Q, Lu X, Zhou H (2016) In situ forming hydrogels based on chitosan for drug delivery and tissue regeneration. Asian J Phar Sci 11(6):673–683.
11. Mondal S, Das S, and Nandi AK (2020) A review on recent advances in polymer and peptide hydrogels. Soft Matter 16(6):1404–1454.
12. Ruiz M, Sastre AM, Guibal E (2000) Palladium sorption on glutaraldehyde-crosslinked chitosan. Reactive & Functional Polymers 45(3):155–173.
13. Özbaş Z, Gürdağ G (2016) Synthesis and Characterization of 5-Fluorouracil-Loaded Glutaraldehyde Crosslinked Chitosan Hydrogels. SDÜ Fen Bilimleri Enstitüsü Dergisi. 20(3):460-467.
14. Pan H, Han JJ, Park YD et al (2016) Effect of sustained release of rhBMP-2 from dried and wet hyaluronic acid hydrogel carriers compared with direct dip coating of rhBMP-2 on peri-implant osteogenesis of dental implants in canine mandibles. Journal of Cranio-Maxillofacial Surgery. 44(2):116-125.
15. Xu C, Ma B, Yua S, Zhao C, Liu H (2020) High-Resolution Patterning of Liquid Metal on Hydrogel for Flexible, Stretchable, and Self-Healing Electronics. Advanced Electronic Materials 6(1):1900721.
16. Liu J, Yang H, Xue Y et al (2020) A Novel Method for Studying the Re-Swelling Capacity of Superabsorbent Polymers in An Artificial Crack. Journal Wuhan University of Technology, Materials Science Edition 35(5):996-1002.
17. Shur M, Akouissi O et al (2023) Revealing the complexity of ultra-soft hydrogel re-swelling inside the brain. Biomaterials 294:122024.
18. Tian H, Cheng S, Zhen J, Lei Z (2023) Superabsorbent Polymer with Excellent Water/Salt Absorbency and Water Retention, and Fast Swelling Properties for Preventing Soil Water Evaporation. J Poly and the Env 31(2):812-824.
19. Lipatova IM, Yusova AA et al (2019) Gelation in solutions of low deacetylated chitosan initiated by high shear stresses. Inter J Bio Macr 139:550-557.
20. Akakuru O, Isiuku B (2017) Chitosan Hydrogels and their Glutaraldehyde-Crosslinked Counterparts as Potential Drug Release and Tissue Engineering Systems - Synthesis, Characterization, Swelling Kinetics and Mechanism. J Phys Chem Biophys 7(3):1-7.
21. Kumirska J, Weinhold MX et al (2011) Biomedical activity of chitin/chitosan-based materials- influence of physicochemical properties apart from molecular weight and degree of N-Acetylation. Polymers 3(4):1875–1901.
22. Kumar MNV (2000) A review of chitin and chitosan applications. Reactive and Functional Polymers 46(1):1-27.
23. Dutta PK, Duta J, Tripathi VS (2004) Chitin and Chitosan: Chemistry, properties and applications. J Sci Ind Res 63(1):20-31.
24. Dash M, Chiellini F et al (2011) Chitosan - A versatile semi-synthetic polymer in biomedical applications. Progress in Polymer Science (Oxford) 36(8):981–1014.
25. Wahba MI (2020) Enhancement of the mechanical properties of chitosan. Journal of Biomaterials Science, Polymer Edition 31(3):350–375.
26. Monteiro O and Airoldi C (1999) Some studies of crosslinking chitosan–glutaraldehyde interaction in a homogeneous system. Int J Bio Macro 26:119–128.
27. Queiroz MF, Melo KRT et al (2015) Does the use of chitosan contribute to oxalate kidney stone formation? Marine Drugs 13(1):141-158.
28. Martínez-Mejía G, Vázquez-Torres NA et al (2019) Synthesis of new chitosan-glutaraldehyde scaffolds for tissue engineering using Schiff reactions. Colloids and Surfaces A: Phys Eng Aspects 579:123658.
29. Kamari A, Pulford ID, Hargreaves JSJ (2011) Chitosan as a potential amendment to remediate metal contaminated soil - A characterisation study. Colloids and Surfaces B: Biointerfaces 82(1):71-80.
30. Sim P, Strudwick XL, Song YM et al (2022) Influence of Acidic pH on Wound Healing In Vivo: A Novel Perspective for Wound Treatment. Int J Mol Sci 23 (21):13655.
31. Mirzaei BE, Ramazani A et al (2013) Studies on glutaraldehyde crosslinked chitosan hydrogel properties for drug delivery systems. Int J Poly Mater and Polym Biomater 62(11), 605–611.
32. Hoffman AS (2012) Hydrogels for biomedical applications. Adv Drug Delivery Rev 64:18–23.
33. Peppas NA, Bures P et al (2000) Hydrogels in pharmaceutical formulations. Eur J Pharm Biopharm 50(1):27-46.
34. Berger J, Reist M et al (2004) Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications. Eur J Pharm Biopharm 57(1):19–34.
35. Shu XZ, Zhu KJ, Song W (2001) Novel pH-sensitive citrate cross-linked chitosan film for drug-controlled release. Int J Pharm 212(1):19-28.
36. Barros SC, da Silva AA B et al (2015) Thermal–mechanical behaviour of chitosan–cellulose derivative thermoreversible hydrogel films. Cellu 22(3):1911–1929.
37. Baloglu E, Karavana SY et al (2011) Rheological and mechanical properties of poloxamer mixtures as a mucoadhesive gel base. Pharm Dev and Tech 16(6):627–636.