The Effects of Boron Toxicity and Bacteria (PGPR) Applications on Growth Development and Physiological Properties in Medicinal Sage (Salvia officinalis L.)

Öz

In this study; it was carried out in order to determine the effects on physiological properties such as total flavonol and chlorophyll amounts, leaf area and temperature with the seedling growth parameters of medicinal sage (Salvia Officinalis) plant of different rhizobacteria (Azospirillum lipoferum, Bacillus megaterium and Frateuria aurentia) and boron doses (0 mM, 5 mM, 10 mM and 20 mM) in a fully controlled climate cabinet in 2021. The experiment was set up in factorial order with 4 replications according to the Completely Randomized Plots Trial Design. In the study, it was investigated growth and development parameters such as root and seedling length (cm), root and seedling fresh weight (g), root and seedling dry weight (g) with physiological parameters such as total flavonol and chlorophyll content (dualex value), leaf area (cm2) and temperature (oC). As a result of the research, While the effects of PGPR applications on root length, seedling fresh weight, seedling length, root fresh and dry weight and leaf area were found to be statistically significant, the effects on seedling dry weight, leaf temperature, flavonol and chlorophyll content were found to be statistically insignificant. Significant increases in growth parameters were noted with rhizobacteria applications compared to control. It was determined that the effect of boron doses on other parameters except leaf temperature was significant. In addition, it was determined that there were decreases in growth parameters with increasing boron doses, but increases in total flavonol and chlorophyll content.

Anahtar Kelimeler

• Seedling growth
• boron doses
• sage
• total flavonol
• total chlorophyll

Tıbbi Adaçayı (Salvia officinalis L.)’ında Bor Toksisitesi ve Rizobakteri (PGPR) Uygulamalarının Fide Gelişimi ve Fizyolojik Özellikleri Üzerine Etkileri

Öz

Bu çalışmada; farklı rizobakteri (Azospirillum lipoferum, Bacillus megaterium ve Frateuria aurentia) ve bor dozlarının (0 mM, 5 mM, 10 mM ve 20 mM) tıbbi adaçayı (Salvia officinalisL.) bitkisinin fide gelişim parametreleri ile toplam flavonol ve klorofil miktarları, yaprak alanı ve sıcaklığı gibi fizyolojik özellikler üzerine etkilerini belirlemek amacıyla tam kontrollü iklim kabininde 2021 yılında yürütülmüştür. Deneme, Tesadüf Parselleri Deneme Deseni’ne göre faktöriyel düzende 4 tekerrürlü olarak kurulmuştur. Araştırmada, kök ve fide uzunluğu (cm), kök ve fide yaş ağırlığı (g), kök ve fide kuru ağırlığı (g) gibi büyüme ve gelişim parametreleri ile toplam flavonol ve klorofil içeriği (dualex değeri), yaprak alanı (cm2) ve sıcaklığı (oC) gibi fizyolojik parametreler incelenmiştir. Araştırma sonucunda; PGPR uygulamalarının kök uzunluğu, fide yaş ağırlığı, fide uzunluğu, kök yaş ve kuru ağırlığı ve yaprak alanı üzerindeki etkisi istatistiksel olarak önemli bulunurken, fide kuru ağırlığı, yaprak sıcaklığı, flavonol ve klorofil miktarı üzerine etkisi istatistiksel olarak önemsiz olmuştur. Rizobakteri uygulamaları ile büyüme parametrelerinde kontrole kıyasla önemli artışların olduğu kaydedilmiştir. Bor dozlarının yaprak sıcaklığı hariç, diğer parametreler üzerindeki etkisinin önemli olduğu tespit edilmiştir. Ayrıca, artan bor dozları ile büyüme parametrelerinde azalmaların olduğu, ancak, toplam flavonol ve klorofil içeriğinde ise artışların olduğu tespit edilmiştir.

Anahtar Kelimeler

• Fide gelişimi
• bor dozları
• adaçayı
• toplam flavonol
• toplam klorofil

Kaynakça

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