Evaluation of Some Phenolic Compounds as Inhibitors of Glutatione S-Transferase Enzyme

Öz

Glutathione S-transferases (GSTs) are important antioxidant enzyme and catalyze the conjugation of glutathione with toxic metabolites. In this study, potential inhibitory effects of some phenolic compounds on the activity of GST enzyme were tested in vitro. The IC50 values of morine hydrate, esculetin hydrate, p-coumaric acid and syringaldehyde phenolic compounds are found as 0.718 μM, 0.713 μM, 0.701 μM, and 0.699 μM, respectively. Ki values are found as 1.610±0.120, 0.179±0.019, 4.590±0.480, 0.999±0.070, respectively. According to these results, esculetin hydrate showed the best inhibition effect and competitive inhibition.

Anahtar Kelimeler

• Glutathione S-transferase
• inhibition
• phenolic compound

Bazı Fenolik Bileşiklerin Glutatyon S-Transferaz Enzim İnhibitörleri Olarak Değerlendirilmesi

Öz

Glutatyon S-transferazlar (GSTs) önemli antioksidan enzim sınıfındadırlar ve glutatyonun toksik metabolitlerle konjugasyonunu katalize ederler. Yapılan bu çalışmada, bazı fenolik bileşiklerin GST enziminin aktivitesi üzerine potansiyel inhibisyon etkileri in vitro olarak test edilmiştir. Morin hidrat, eskuletin hidrat, p-kumarik asit ve siringaldehit fenolik bileşiklerinin GST enzimi üzerine IC50 değerleri sırasıyla 0.718 μM, 0.713 μM, 0.701 μM ve 0.699 μM olarak bulunmuştur. Ki değerleri ise sırasıyla 1.610±0.120, 0.179± 0.019, 4.590±0.480, 0.999±0.070 olarak bulunmuştur. Bu sonuçlara göre en iyi inhibisyon etkisini eskuletin hidrat göstermiş olup yarışmalı inhibisyon türü sergilemiştir.

Anahtar Kelimeler

• Glutatyon S-transferaz
• inhibisyon
• fenolik bileşik

Kaynakça

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