Bal Arılarının Sindirim Kanalında Bulunan Probiyotik Kökenli Bifidobacterium sp.’nin Enzimatik ve Bibliyometrik Analizi

Öz

Bal arıları (Apis mellifera) bal, polen, arısütü, propolis, bal mumu ve arı zehiri gibi ürünleri üreten ve birçok doğal ve endüstriyel bitkinin tozlaşmasında vektör işlevi gören eşsiz bir süper organizmadır. Son zamanlarda meydana gelen koloni kayıpları sebepleri arasında bağırsak mikroflorasının etkisi ve buna bağlı olarak bağışıklık sistemi ön plana çıkmaktadır. Arıların sindirim tüpünde bulunan bakterilerin çeşitliliği ve yoğunlukları sonucu bağışıklık sistemleri etkilenebilmektedir. Bu etkinin olumlu yönde olabilmesi için bağırsak mikroflorasının doğal ve dengeli olması gerekmektedir. Bağırsakta bulunan bakteri popülasyonları arasında önemli bir yere sahip olan probiyotik kökenli Bifidobacterium sp.’nin sahip olduğu enzim aktivitesi hem diğer simbiyont mikroorganizmalar üzerinde hem de konakçının beslenmesinde kritik bir etkiye sahiptir. Bundan dolayı bu çalışmamızda Bifidobacterium sp.’nin önemini vurgulamak için enzim aktivitesi ve bibliyometrik analizler yapılmıştır. Optimum glikozit hidrolaz enzim aktivitesinin hesaplanması için farklı pH, sıcaklık ve substratlar tercih edilmiştir. Enzim aktivitesinin analizi sonucu Bifidobacterium sp.’nin glikozit hidrolaz etkinliği (pH 5.0 ve 30oC) tespit edilmiştir. İnülin substratının daha fazla kullanılması probiyotik Bifidobacterium sp. için doğal prebiyotik kaynağı olduğunu göstermiştir. Aynı zamanda yeni nesil prebiyotik kaynağı olarak bilinen ksilanın hidrolizi de gerçekleşmiştir. Bibliyometrik analiz için Web Of Science ile Scopus veri tabanındaki korelasyon bağlantıları VOS viewer yazılımı yardımıyla sonuçlandırılmıştır.

Anahtar Kelimeler

• Apis mellifera
• Bifidobacterium
• Probiyotik
• Bibliyometrik Analiz
• Enzim Aktivitesi

Enzymatic and Bibliometric Analysis of Bifidobacterium sp. of Probiotic Origin in The Based Digestive Tract of Honey Bees

Öz

Honey bees (Apis mellifera) are a unique super organism that produces products such as honey, pollen, royal jelly, propolis, beeswax and bee venom and serves as a vector for pollination of many natural and industriyal plants. The effect of intestinal microflora and, accordingly, the immune system come to the fore among the causes of colony losses that have occurred recently. The diversity and densities of bacteria in the digestive tube of bees are affected by their immune systems. In order for this effect to be positive, the intestinal microflora must be natural and balanced. The enzyme activity of the probiotic-derived Bifidobacterium sp., which has an important place among the bacterial populations in the intestine, has a critical effect on both other symbiont microorganisms and the nutrition of the host. Therefore, in this study, both glikozide hidrolase enzyme activity analysis and bibliometric analysis were performed to emphasize the importance of Bifidobacterium sp. Different pH, temperature and substrates were preferred to calculate the optimum enzyme activity. As a result of the analysis of the enzyme activity, the glycoside hydrolase activity (pH 5.0 and 30oC) of Bifidobacterium sp. was determined. Further use of inulin substrate has shown that it is a natural prebiotic source for this probiotic microorganism. At the same time, the hydrolysis of xylan, which is known as a new generation prebiotic source, has also taken place. For bibliometric analysis, correlation links in Web of Science and Scopus database were concluded with the help of VOS viewer software

Anahtar Kelimeler

• Apis mellifera
• Bifidobacterium
• probiotic
• Bibliometric Analysis
• Enzyme Activity

Kaynakça

1. Alatawy M, Al-Attas SG, Assagaf AI, Al-Shehri A, Alghamdi KM, Bahieldin A, 2020. Gut microbial communities of adult honey bee workers (apis mellifera). Biosciences Biotechnology Research Asia, 17(2), 353-362.
2. Alberoni D, Baffoni L, Gaggìa F, Ryan PM, Murphy K, Ross PR, Stanton C, Di Gioia D, 2018. Impact of beneficial bacteria supplementation on the gut microbiota, colony development and productivity of apis mellifera l. Beneficial microbes, 9(2), 269-278.
3. Aldemir S, Tunca Rİ, Topal E, Margaoan R, 2019. Bal arılarının bağırsak yapısına etki eden faktörler. Arıcılık Araştırma Dergisi, 11(1), 28-34.
4. Anonim, 2022a. Bal analizi, sindirim kanalında bulunan simbiyotik bifidobacterium analizi, türkiye’nin bal arıları ve mikrobiyota konuları üzerine yapılan araştırmaların analizi raporları https://wcs.webofknowledge.com/RA/analyze.do (Erişim Tarihi: 14.02.2022).
5. Anonim, 2022b. Bal analizi, sindirim kanalında bulunan simbiyotik bifidobacterium analizi, türkiye’nin bal arıları ve mikrobiyota konuları üzerine yapılan araştırmaların analizi raporları https://www.scopus.com/results (Erişim Tarihi: 14.02.2022).
6. Aziz G, Tariq M, Zaidi AH, 2021. Mining indigenous honey bee gut microbiota for lactobacillus with probiotic potential. Microbiology, 001032.
7. Baffoni L, Gaggia F, Alberoni D, Cabbri R, Nanetti A, Biavati B, Di Gioia D, 2016. Effect of dietary supplementation of bifidobacterium and lactobacillus strains in apis mellifera l. against nosema ceranae. Beneficial microbes 7 (1): 45–51.
8. Blaut M, 2002. Relationship of prebiotics and food to intestinal microflora. European journal of nutrition, 41(1), i11-i16.

9. Boğ EŞ, Ertürk Ö, Yaman M, 2020. Pathogenicity of aerobic bacteria isolated from honeybees (apis mellifera) in ordu province. Turkish Journal of Veterinary and Animal Sciences, 44(3), 714-719.
10. Borges D, Guzman-Novoa E, Goodwin PH, 2020. Control of the microsporidian parasite nosema ceranae in honey bees apis mellifera using nutraceutical and immuno-stimulatory compounds. Plos one, 15(1), e0227484.
11. Borges D, Guzman-Novoa E, Goodwin PH, 2021. Effects of prebiotics and probiotics on honey bees (apis mellifera) infected with the microsporidian parasite nosema ceranae. Microorganisms, 9(3), 481.
12. Cheng G, Varanasi P, Li C, Liu H, Melnichenko YB, Simmons BA, Kent MS, Singh S, 2011. Transition of cellulose crystalline structure and surface morphology of biomass as a function of ionic liquid pretreatment and its relation to enzymatic hydrolysis. Biomacromolecules, 12(4), 933-941.
13. Daisley BA, Chmiel JA, Pitek AP, Thompson GJ, Reid G, 2020. Missingmicrobes in bees: how systematic depletion of key symbionts erodes immunity. Trends in Microbiology.
14. de Melo FHC, Menezes FNDD, de Sousa JMB, dos Santos Lima M, Borges GDSC, de Souza EL, Magnani M, 2020. Prebiotic activity of monofloral honeys produced by stingless bees in the semi-arid region of brazilian northeastern toward lactobacillus acidophilus LA-05 and bifidobacterium lactis bb-12. Food Research International, 128, 108809.
15. Delgado S, Guadamuro L, Flórez AB, Vázquez L, Mayo B, 2019. Fermentation of commercial soy beverages with lactobacilli and bifidobacteria strains featuring high β-glucosidase activity. Innovative Food Science&Emerging Technologies, 51, 148–155.
16. Engel P, Martinson VG, Moran NA, 2012. Functional diversity within the simple gut microbiota of the honey bee. Proceedings of the National Academy of Sciences, 109(27), 11002-11007.
17. Fries I, Morse R, Flottum K, 1997. Honey bee pests, predators, and diseases. Protozoa. Medina, Ohio, USA: AI Root Company, 59.
18. Garcia-Mazcorro JF, Kawas JR, Marroquin-Cardona AG, 2019. Descriptive bacterial and fungal characterization of propolis using ultra-high-throughput marker gene sequencing. Insects, 10(11), 402.
19. Geldert C, Abdo Z, Stewart JE, Arathi HS, 2021. Dietary supplementation with phytochemicals improves diversity and abundance of honey bee gut microbiota. Journal of Applied Microbiology, 130(5), 1705-1720.
20. Gill RJ, Baldock KC, Brown MJ, Cresswell JE, Dicks LV, Fountain MT, Garratt MPD, Gough LA, Heard MS, Holland JM, Ollerton J, Stone GN, Tang CQ, Vanbergen AJ, Vogler AP, Woodward G, Arce AN, Boatman ND, Brand-Hardy R, Breeze TD, Green M, Hartfield CM, O’Connor RS, OsborneJL, Phillips J, Sutton PB, 2016. Protecting an ecosystem service: approaches to understanding and mitigating threats to wild insect pollinators. AdvEcoRes 54:135–206. doi:10.1016/bs.aecr.2015.10.007
21. Ilijević K, Vujanović D, Orčić S, Purać J, Kojić D, Zarić N, Grzetić I, Blagojević DP, Čelić, TV, 2021. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. Comparative biochemistry and physiology part c:
22. Kerr JT, Pindar A, Galpern P, Packer L, Potts SG, Roberts SM, Rasmont P, Schweiger O, Colla SR, Richardson LL, Wagner DL, Gall LF, Sikes DS, Pantoja, A, 2015. Climate change impacts on bumblebees converge across continents. Science, 349(6244), 177-180.
23. Kolida S, Tuohy K, Gibson GR, 2002. Prebiotic effects of inulin and oligofructose. British Journal of Nutrition, 87(S2), S193-S197.
24. Konanç K, Ozturk E, 2020. Use of propolis as a digestive system regulator in poultry.Proceedıng Book, 131.
25. Korakli M, Gänzle MG, Vogel RF, 2002. Metabolism by bifidobacteria and lactic acid bacteria of polysaccharides from wheat and rye, and exopolysaccharides produced by lactobacillus sanfranciscensis. Journal of applied microbiology, 92(5), 958-965.
26. Kutlu MA, 2020. Bingöl ilinde yaşanan koloni kayıpları (arı ölümleri), nedenleri ve öneriler. Türk Tarım ve Doğa Bilimleri Dergisi, 7(4), 867-873.
27. Kutlu MA, Gul A, 2021. Siirt ili pervari ilçesi arıcılığı, hastalıklarının tanınırlıkları ve koloni kayıpları üzerine bir çalışma. Adyutayam Dergisi, 9(1), 13-21.
28. Kwong WK, Medina LA, Koch H, Sing KW, Soh EJY, Ascher JS, Jaffé R, Ve Moran NA, 2017. Dynamic microbiome evolution in social bees. Science Advances, 3(3), e1600513.
29. Kwong WK, Moran NA, 2016. Gut microbial communities of social bees. Nature Reviews Microbiology, 14(6), 374-384.
30. Ludvigsen J, Andersen A, Hjeljord L, Rudi K, 2021. The honey bee gut mycobiota cluster by season versus the microbiota which cluster by gut segment. Veterinary sciences, 8(1), 4.
31. Martinson VG, Moy J, Moran NA, 2012. Establishment of characteristic gut bacteria during development of the honeybee worker. Applied and environmental microbiology, 78(8), 2830-2840.
32. Miller GL, 1959. Use of dinitrosalicylicacid reagent for determination of reducing sugar. Analytical chemistry 31(3) : 426-428.
33. Moran NA, Hansen AK, Powell JE, Sabree ZL, 2012. Distinctive gut microbiota of honey bees assessed using deep sampling from individual worker bees. PloSone, 7(4), e36393.
34. Panjad P, Yongsawas R, Sinpoo C, Pakwan C, Subta P, Krongdang S,In-on A, Chomdej S,Chantawannakul P, Disayathanoowat T, 2021. Impact of nosema disease and american foulbrood on gut bacterial communities of honey bees apis mellifera. Insects, 12(6), 525.
35. Qiu XJ, Zheng WX, Zhang L, Shi YL, Hu JH, Li Y, Liu ZY, Zhu MD, 2020. Prebiotic effects of xylanase modification of?-glucan from oat bran on bifidobacterium bifidum. Italian Journal of Food Science, 32(1).
36. Raymann K, 2021. Honey bee microbiota and the physiology of antimicrobial resistance. Honey Bee Medicine for the Veterinary Practitioner, 125-134.
37. Raymann K, Bobay LM, Moran NA, 2018. Antibiotics reduce genetic diversity of core species in the honey bee gut microbiome. Molecular ecology, 27(8), 2057-2066.
38. Salas-Veizaga DM, Bhattacharya A, Adlercreutz P, Stalbrand H, Karlsson EN, 2021. Glucuronosylated and linear xylooligosaccharides from quinoa stalks xylan as potential prebiotic source for growth of bifidobacterium adolescentis and weissella cibaria. LWT, 152, 112348.
39. Sánchez-Portilla Z, Melgoza-Contreras LM, Reynoso-Camacho R, Pérez-Carreón JI, Gutiérrez-Nava A, 2020. Incorporation of bifidobacterium sp. into powder products through a fluidized bed process for enteric targeted release. Journal of Dairy Science, 103(12), 11129-11137.
40. Scourboutakos, M. 2010. 1+ 1= 3… Synbiotics: Combining the power of pre‐and probiotics. Journal of Food Science Education, 9(1), 36-37.
41. Seeley TD, 1989. Social foraging in honey bees: how nectar foragers assess their colony's nutritional status. Behavioral Ecology and Sociobiology, 24(3), 181-199.
42. Seeley TD, 1989. The honey bee colony as a superorganism. American Scientist, 77(6), 546-553.
43. Söğüt B, Şeviş HE, Karakaya E, İnci H, Yılmaz HŞ, 2019. Bingöl ilinde arıcılık faaliyetinin mevcut yapısı üzerine bir araştırma. Türk Tarım ve Doğa Bilimleri Dergisi, 6(2): 168- 177.
44. Suyabatmaz Ş, Bozdeveci A, Karaoğlu ŞA, 2020. Bal arılarında gastrointestinal bakteriyel flora.
45. Traynor KS, Rennich K, Forsgren E, Rose R, Pettis J, Kunkel G, Lopez D, Madella S, Evans J, vanEngelsdorp D, 2016. Multi year survey targeting disease incidence in us honeybees. apidologie, 47(3), 325-347.
46. TÜİK, 2021. Türkiye istatistik kurumu, tüik http://www.tüik.gov.tr
47. Weese JS, 2002. Probiotics, prebiotics, and synbiotics. Journal of equine veterinary science, 22(8), 357-360.
48. Yazdıç F, Yazdıç FC, Kar B, 2020. Ziraat çalışmaları ve çiftlik hayvanlarında ileri biyoteknolojik uygulamalar, İksad Yayınevi s.49-74, Ankara-Turkey.
49. Zeybek N, Rastall RA, Buyukkileci AO, 2020. Utilization of xylan-type polysaccharides in co-culture fermentations of bifidobacterium and bacteroides species. Carbohydrate polymers, 236, 116076.
50. Zheng H, Steele MI, Leonard SP, Motta EV, Moran NA, 2018. Honey bees as models for gut microbiota research. Lab animal, 47(11), 317-325.