Tavşan Plazmasında Naproksenin GC-MS Yöntemi ile Analizi

Öz

Naproksen günümüzde en çok kullanılan ilaçlardan biridir. Bu nedenle, naproksen tayini için yeni ve basit yöntemlerin geliştirilmesi önemlidir. Bu çalışmanın amacı tavşan plazmasında naproksenin analizi için bir gaz kromatografisi-kütle spektrometri yöntem geliştirmek ve farmakokinetik çalışmaya uygulamaktır. Tavşan kan örnekleri sıvı-sıvı ekstraksiyon yöntemi ile hazırlanmıştır. Naproksenin ayırımı HP-5 MS kolon ile yapılmıştır. Yöntemin kalibrasyon eğrisi 0.1 ve 5.0 μg mL-1 arasında çizildi. Tavşan plazmasında naproksenin kesinlik sonuçları %4.17’den küçüktü ve doğruluk sonuçları %2.18’den daha iyiydi. Yöntemin tavşan plazmasındaki tüm örnekler için geri kazanım değerleri >%94.2’tür. Ayrıca, tavşanlarda naproksenin farmakokinetiğini incelemek için valide edilen yöntem uygulanmıştır. Maksimum naproksen plazma konsantrasyonu 42.1±4.243 μg mL-1 idi. Naproksenin maksimum konsantrasyona ulaşma süresi ve eğrinin altındaki alan (AUC0-16 h) sırasıyla 1.50±0.196 h ve 566.3±41.72 μg mL-1 idi.

Anahtar Kelimeler

• GC-MS
• ekstraksiyon
• naproksen
• farmakokinetik
• tavşan

Analysis of Naproxen in Rabbit Plasma by GC-MS Method

Öz

Naproxen is one of the most used drugs today. Therefore, it is important to develop new and simple methods for the determination of naproxen. The goal of this research is to develop a gas chromatography-mass spectrometry method for analyzing naproxen levels in rabbit plasma and apply this method to the pharmacokinetic study. The liquid-liquid extraction technique was used to prepare blood samples from rabbits. Separation of naproxen was achieved on an HP-5 MS column. The method’s calibration curve was plotted between 0.1 and 5.0 μg mL-1. The accuracy results were better than 2.18% and the precision results were less than 4.17% in rabbit plasma for naproxen. The method had recovery values >94.2% for all samples in rabbit plasma. In addition, the validated method was used to study naproxen pharmacokinetics in rabbits. The maximum naproxen plasma concentration is 42.1±4.243 μg mL-1. The duration to attain the greatest naproxen concentration and the area under the curve from (AUC0-16 h) were 1.50±0.196 h and 566.3±41.72 μg mL-1 h.

Anahtar Kelimeler

• GC-MS
• extraction
• naproxen
• pharmacokinetic
• rabbit

Kaynakça

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