Effects of Exogenous Linoleic Acid on Barley (Hordeum vulgare L.) Seedlings Under Salinity

Yıl 2022, , 1790 - 1800, 01.09.2022

Cüneyt Uçarlı

https://doi.org/10.21597/jist.1105133

Cited By: 1

Öz

Salt stress adversely affects plants and causes different levels of morphological,
physiological, biochemical, and molecular changes at different growth stages. Polyunsaturated fatty
acids (PUFAs), such as linoleic acid, are main components of membrane lipids and determine the
fluidity and stability of the cell membrane. In addition, PUFAs have a crucial role in maintaining the
structure and function of the cell membrane which is damaged by salinity. There may be a relationship
between level of PUFAs in membrane lipids and salinity tolerance. The present study was carried out
to examine the effects of exogenous application of 0.5 mM linoleic acid (LA) on barley seedlings
(Hordeum vulgare L. cv. Martı) grown in hydroponic conditions under 160 mM NaCl. The treatment
with LA ameliorated the stress generated by NaCl by increasing osmolyte level and decreasing ion
leakage percentage and H2O2 content within hours. Besides, LA significantly enhanced expression of
salt-responsive transcription factor HvDRF2 and ROS scavenger gene HvMT2 as 105- and 40-fold,
respectively, in the leaves of barley seedlings under salinity conditions. While LA slightly increased
the gene expression of ascorbate peroxidase (HvAPX), glutathione S-transferase (HvGST6) and copper
zinc superoxide dismutase (HvCu/ZnSOD) in the roots of barley seedlings, the expression of these
genes was not changed in the leaves under salinity compared to salt-stressed samples. This study
provides novel insights for effects of LA on improvement of salinity tolerance in barley.

Anahtar Kelimeler

NaCl, linoleic acid, ion leakage, osmolality, HvDRF2, HvMT2

Proje Numarası

23966

Kaynakça

• Aziz A, Siti-Fairuz M, Abdullah MZ, Ma NL, Marziah M, 2015. Fatty acid profile of salinity tolerant rice genotypes grown on saline soil. Malaysian Applied Biology, 44: 119–124.
• Bienert GP, Møller AL, Kristiansen KA, Schulz A, Møller IM, Schjoerring JK, Jahn TP, 2007. Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes. Journal of Biological Chemistry, 282(2): 1183-92.
• Bose J, Rodrigo-Moreno A, Shabala S, 2014. ROS homeostasis in halophytes in the context of salinity stress tolerance. Journal of Experimental Botany, 65: 1241–1257.
• Carillo P, Mastrolonardo G, Nacca F, Parisi D, Verlotta A, Fuggi A, 2008. Nitrogen metabolism in durum wheat under salinity: accumulation of proline and glycine betaine, Functional Plant Biology, 35(5): 412-426.
• Chen Z, Pottosin II, Cuin TA, Fuglsang AT, Tester M, Jha D, Zepeda-Jazo I, Zhou M, Palmgren MG, Newman IA, Shabala S, 2007 Root plasma membrane transporters controlling K+/Na+ homeostasis in salt-stressed barley. Plant Physiology, 145(4): 1714–1725.
• Chen T, Shabala S, Niu Y, Chen ZH, Shabala L, Meinke H, Venkataraman G, Pareek A, Xu J, Zhou M, 2021, Molecular mechanisms of salinity tolerance in rice, The Crop Journal. 9(3): 506-520.
• FAO. (2020) FAOSTAT statistical database. http://www.fao. org/faostat/en/#data/QC. (Date of access: 16 March 2022).
• Gill SS, Tuteja N, 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48: 909–930.
• Gogna M, Bhatla SC, 2020. Salt-tolerant and -sensitive seedlings exhibit noteworthy differences in lipolytic events in response to salt stress. Plant Signaling & Behavior, 15(4): 1737451.
• Golldack D, Lüking I, Yang O, 2011. Plant tolerance to drought and salinity: stress regulating transcription factors and their functional significance in the cellular transcriptional network. Plant Cell Reports, 30: 1383–91.
• Guo G, Dondup D, Yuan X, Gu F, Wang D, Jia F, Lin Z, Baum M, Zhang J, 2014. Rare allele of HvLox-1 associated with lipoxygenase activity in barley (Hordeum vulgare L .). Theoretical and Applied Genetics, 127(10): 2095-103.
• Gupta B, Huang B, 2014. Mechanism of salinity tolerance in plants: Physiological, biochemical, and molecular characterization. International Journal of Genomics, Article ID 701596.
• Hassinen VH, Tervahauta AI, Schat H, Karenlampi SO, 2011. Plant metallothioneins–metal chelators with ROS scavenging activity? Plant Biology, 13: 225-232.
• He M, Ding N-Z, 2020. Plant Unsaturated Fatty Acids: Multiple Roles in Stress Response. Frontiers in Plant Science. 11: 562785.
• Hu L, Li H, Pang H, Fu J, 2012. Responses of antioxidant gene, protein and enzymes to salinity stress in two genotypes of perennial ryegrass (Lolium perenne) differing in salt tolerance. Journal of Plant Physiology, 169: 146–56.
• Jiang Q, Hu Z, Zhang H, Ma Y, 2014. Overexpression of GmDREB1 improves salt tolerance in transgenic wheat and leaf protein response to high salinity. The Crop Journal, 2: 120–131.
• Joshi R, Pareek A, Singla-Pareek SL, 2016. Plant metallothioneins: classification, distribution, function, and regulation. P. Ahmad (Ed.), Plant Metal Interaction, Elsevier (2016), pp. 239-261.
• Kaya C, Sonmez O, Aydemir S, Ashraf M, Dikilitaş M, 2013. Exogenous application of mannitol and thiourea regulates plant growth and oxidative stress responses in salt-stressed maize (Zea mays L.). Journal of Plant Interactions, 8: 234–241.
• Mahlooji M, Seyed Sharifi R, Razmjoo J, SAbzalian MR, Sedghi M, 2018. Effect of salt stress on photosynthesis and physiological parameters of three contrasting barley genotypes. Photosynthetica, 56: 549–556.
• Mittler R, 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Scince, 9: 405-10.
• Munns R, Tester M, 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology,59: 651–681.
• Nayak SN, Balaji J, Upadhyaya HD, Hash CT, Kishor PBK, Chattopadhyay D, et al., 2009. Isolation and sequence analysis of DREB2A homologues in three cereal and two legume species. Plant Science, 177: 460–467.
• Parida AK, Das AB, 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60: 324–49.
• Rosahl S, 1996. Lipoxygenases in plants--their role in development and stress response. Zeitschrift für Naturforschung C, 51(3-4): 123-38.
• Seckin B, Turkan I, Sekmen AH, Ozfidan C, 2010. The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds. (sea barleygrass) and Hordeum vulgare L. (cultivated barley). Environmental and Experimental Botany, 69: 76–85.
• Shagimardanova EI, Gusev OA, Sychev VN, Levinskikh MA, Sharipova MR, Il'inskaia ON, Bingham G, Sugimoto M, 2010. Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment. Molecular Biology, 44: 734–740.
• Sharma P, Jha AB, Dubey RS, Pessarakli M, 2012. Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions. Journal of Botany, Article ID 217037.
• Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002) Regulation and function of ascorbate peroxidase isoenzymes. Journal of Experimental Botany, 53(372): 1305–1319.
• Singhal RK, Saha D, Skalicky M, Mishra UN, Chauhan J, Behera LP, Lenka D, Chand S, Kumar V, Dey P, Indu, Pandey S, Vachova P, Gupta A, Brestic M, El Sabagh A, 2021. Crucial Cell Signaling Compounds Crosstalk and Integrative Multi-Omics Techniques for Salinity Stress Tolerance in Plants. Frontiers in Plant Science, 12: 670369.
• Singh M, Kumar J, Singh S, Singh VP, Prasad SM, 2015. Roles of osmoprotectants in improving salinity and drought tolerance in plants: a review. Reviews in Environmental Science and Bio/Technology, 14: 407–426.
• Sui N, Wang Y, Liu S, Yang Z, Wang F, Wan S, 2018. Transcriptomic and Physiological Evidence for the Relationship between Unsaturated Fatty Acid and Salt Stress in Peanut. Frontiers in Plant Science, 9: 7.
• Sumayo MS, Kwon DK, Ghim SY, 2014. Linoleic acid-induced expression of defense genes and enzymes in tobacco. Journal of Plant Physiology, 171: 1757–1762.
• Uçarlı C, Gürel F, 2020. Differential physiological and molecular responses of three-leaf stage barley (Hordeum vulgare L.) under salt stress within hours. Plant Biotechnology Reports, 14: 89–97.
• Uçarlı C, McGuffin LJ, Çaputlu S, Aravena A, Gürel F, 2016. Genetic diversity at the Dhn3 locus in Turkish Hordeum spontaneum populations with comparative structural analyses. Scientific Reports, 6: 20966.
• Ueda A, Kathiresan A, Inada M, Narita Y, Nakamura T, Shi W et al., 2004. Osmotic stress in barley regulates expression of a different set of genes than salt stress does. Journal of Experimental Botany, 55: 2213-2218.
• Viswanath KK, Varakumar P, Pamuru RR, Basha SJ, Mehta S, Rao AD, 2020. Plant lipoxygenases and their role in plant physiology. Journal of Plant Biology, 63: 83–95.
• Walia H, Wilson C, Wahid A, Condamine P, Cui X, Close TJ, 2006. Expression analysis of barley (Hordeum vulgare L.) during salinity stress. Functional and Integrative Genomics, 6: 143–156.
• Wani SH, Kumar V, Khare T, Guddimalli R, Parveda M,Solymosi K, et al.,2020. Engineering salinity tolerance in plants: progress and prospects. Planta, 251: 1–29.
• Wu D, Shen Q, Cai S, Chen ZH, Dai F, Zhang G, 2013. Ionomic responses and correlations between elements and metabolites under salt stress in wild and cultivated barley. Plant and Cell Physiology, 54: 1976–1988.
• Xue T, Li X, Zhu W, Wu C, Yang G, Zheng C, 2009. Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast. Journal of Experimental Botany, 60: 339–349.
• Yin Y, Jiang X, Ren M, Xue M, Nan D, Wang Z, Xing Y, Wang M, 2018. AmDREB2C, from Ammopiptanthus mongolicus, enhances abiotic stress tolerance and regulates fatty acid composition in transgenic Arabidopsis. Plant Physiology and Biochemistry, 130: 517–528.
• Zhang H, Han B, Wang T, Chen S, Li H, Zhang Y, Dai S, 2012. Mechanisms of Plant Salt Response : Insights from Proteomics. Journal of Proteome Research,11:49–67.
• Zhao FG, Qin P, 2005. Protective effects of exogenous fatty acids on root tonoplast function against salt stress in barley seedlings. Environmental and Experimental Botany, 53: 215-223.