Molecular Identification and Determination of Antimicrobial Activity of Micromonospora and Nonomuraea Isolates

Öz

Actinobacteria are the largest member of the Bacteria group, which can live in different ecosystems and create a rich source of secondary metabolites. Rare actinobacteria have become one of the important targets in terms of active metabolites. Micromonospora and Nonomuraea, which are rare Actinobacteria, are among the genera that have attracted the most attention recently. In the present study, it was aimed to isolate Micromonospora and Nonomuraea genus members from an endemic species. 16S rRNA gene region analyzes were used for molecular identification of isolates. As a result of the analysis, it was determined that 10 isolates were members of Micromonospora and 2 isolates were members of Nonomuraea. It has been determined that Nonomuraea sp. HCI 01 and HCI 02 strains and Micromonospora sp. HCI 04, HCI 23, HCI 39, HCI 44 and HCI 49 strains have the potential to become a possible new species. In biological activity studies, Micromonospora sp. HCI 45, HCI 47 and HCI 49 strains were found to be active against E. coli, HCI 20 and HSF 02 against S aureus, and HCI 04 and HSF 02 against S. cerevisiae. Nonomuraea sp. HCl 02 was found to have antimicrobial activity against S. cerevisiae. The results obtained have shown that there are possible new species and the existence of biologically active molecules with the potential to be antibiotics.

Anahtar Kelimeler

• 16S rRNA
• antimicrobial activity
• Micromonospora
• Nonomuraea

Micromonospora ve Nonomuraea İzolatlarının Moleküler Tanımlamaları ve Antimikrobiyal Aktivitelerinin Belirlenmesi

Öz

Aktinobakteriler farklı ekosistemlerde yaşayabilen ve sekonder metabolit açısından zengin kaynak oluşturan bakteri grubunun en büyük üyesini oluşturmaktadır. Nadir aktinobakteriler ise aktif metabolitler açısından önemli hedeflerden biri haline gelmiştir. Nadir aktinobakterilerden olan Micromonospora ve Nonomuraea son dönemde en çok ilgi çeken cinslerdendir. Sunulan çalışmada endemik bir türden Micromonospora ve Nonomuraea cins üyelerinin izolasyonu hedeflenmiştir. İzolatların moleküler tanımlamalarında 16S rRNA gen bölgesi analizleri kullanılmıştır. Analizler sonucunda 10 izolatın Micromonospora üyesi, 2 izolatın Nonomuraea üyesi olduğu belirlenmiştir. Nonomuraea sp. HCI 01 ve HCI 02 suşları Micromonospora sp. HCI 04, HCI 23, HCI 39, HCI 44 ve HCI 49 suşlarının olası yeni birer tür olma potansiyeline sahip olduğu tespit edilmiştir. Antimikrobiyal aktivite çalışmalarında Micromonospora sp. HCI 45, HCI 47 ve HCI 49 suşları E. coli’ye karşı, HCI 20 ve HSF 02 S. aureus’a karşı ve HCI 04 ve HSF 02 ise S. cerevisiae’a karşı etkinlik gösterdiği belirlenmiştir. Nonomuraea sp. ise HCI 02 S. cerevisiae’a karşı antimikrobiyal aktiviteye sahip olduğu görülmüştür. Elde edilen sonuçlarla, olası yeni türlerin olduğu ve antibiyotik olma potansiyeline sahip biyolojik aktif moleküllerin varlığı gösterilmiştir.

Anahtar Kelimeler

• 16S rRNA
• antimikrobiyal aktivite
• Micromonospora
• Nonomuraea

Kaynakça

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