Fenamifos’un Sıçan Kan ve Dalak Dokusunda Sebep Olduğu Oksidatif Stres Üzerine Naringenin’in Koruyucu Rolü

Öz

Organofosfatlı bir insektisit olan fenamifos, tarım alanlarında sıklıkla kullanılmakta, çevre ve halk sağlığı açısından çeşitli sorunlar meydana getirmektedir. Naringenin, antikanser, antiinflamatuvar, antioksidan ve antiproliferatif aktivitelere sahip bir flavonondur. Bu çalışmanın amacı sıçanların kan ve dalak dokusunda fenamifos kaynaklı oksidatif stres üzerine naringeninin koruyucu rolünü araştırmaktır. Bu çalışmada sıçanlar her grupta 6 adet olacak şekilde 4 gruba ayrılmıştır. 1. grup: kontrol grubu, 2. grup: fenamifos (0.76 mg/kg v.a.) muameleli grup, 3. grup: naringenin (50 mg/kg v.a.) muameleli grup 4. grup: fenamifos (0.76 mg/kg v.a.) ve naringenin (50 mg/kg v.a.) muameleli grup. Maddeler deney hayvanlarına 28 gün boyunca gavaj yolu ile verilmiştir. Fenamifos muameleli grup, kontrol grubu ile karşılaştırıldığında MDA, IL-17 ve 8-OHdG düzeylerinde kontrol grubuna göre istatistiksel olarak anlamlı bir artış gözlenirken (p<0.05), SOD, CAT, GPx ve GST enzim aktivitelerinde ise istatiksel olarak anlamlı bir azalma gözlenmiştir (p<0.05). Naringenin muameleli grup, fenamifos muameleli grup ile karşılaştırıldığında, parametrelerde anlamlı bir iyileşme gözlenmiştir. Bu çalışmada fenamifosun sıçanların kan ve dalak dokusunda oluşturduğu toksisiteye karşı, antioksidan özellikleri güçlü naringenin uygulamasının oksidatif hasarı önemli ölçüde azalttığı gözlenmiştir.

Anahtar Kelimeler

• Kan
• Dalak
• Fenamifos
• Naringenin
• Oksidatif stres

Protective Role of Naringenin on Oxidative Stress Caused by Fenamiphos in Rat Blood and Spleen Tissue

Öz

Fenamiphos, an organophosphate insecticide, is frequently used in agricultural areas and causes various problems in terms of environment and public health. Naringenin is a flavonone with anticancer, anti-inflammatory, antioxidant and antiproliferative activities. The aim of this study is to investigate the protective role of naringenin on fenamiphos-induced oxidative stress in the blood and spleen tissue of rats. In this study, rats were divided into 4 groups, with 6 in each group. 1st group: control group, 2nd group: fenamifos (0.76 mg/kg b.w.) treated group, 3rd group: naringenin (50 mg/kg b.w.) treated group 4th group: fenamifos (0.76 mg/kg b.w.) v.a.) plus naringenin (50 mg/kg b.a.) treated group. The substances were given to experimental animals via gavage for 28 days. When the fenamiphos treated group was compared with the control group, a statistically significant increase was observed in MDA, IL-17 and 8-OHdG levels compared to the control group (p <0.05), while a statistically significant decrease was observed in SOD, CAT, GPx and GST enzyme activities (p<0.05). When the naringenin-treated group was compared with the fenamiphos-treated group, a significant improvement in the parameters was observed. In this study, it was observed that the administration of naringenin, which has strong antioxidant properties, significantly reduced oxidative damage against the toxicity caused by fenamiphos in the blood and spleen tissue of rats.

Anahtar Kelimeler

• Blood
• Spleen
• Fenamiphos
• Naringenin
• Oxidative stress

Kaynakça

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