Preparation, characterization and in vitro applications of morin hydrate loaded HPAC@MNPs nanocomposite

Öz

Cancer is a prevalent and fatal illness that claims the lives of millions of people globally every year, impacting individuals across all age groups and ethnicities. Because drugs that are used to treat cancer affect both malignant and healthy cells without discrimination, they are responsible for systemic toxicity in addition to creating major adverse effects Recently, drug delivery systems that specifically target specific sites have been developed to minimize adverse effects caused by drugs. Morin (MRN) is a flavonol-based drugs that has been the subject of substantial research that has been conducted to investigate its antiangiogenic, anti-inflammatory, anticancer, and antibacterial activities. This study included the synthesis of a product with magnetic properties by applying a layer of magnetic nanoparticles onto biocompatible activated carbon derived from the hawthorn plant. Characterization of HPAC@MNPs synthesized with magnetic nanoparticle (MNPs) of activated carbon (AC) obtained from hawthorn plant (HP) was confirmed by Fourier Transform Infrared (FTIR), Scanning Electron Microscope (SEM), dynamic light scattering (DLS) and Zeta Potential. DLS analysis calculated the average particle size of HPAC@MNPs and HPAC@MNPs-MRN to be about 105 nm and 142 nm, respectively. The drug-loaded magnetic nanocomposite was evaluated for its cytotoxic effects on MCF-7 (breast), U373 (Glioblastoma) cancer cell lines, and Human Umbilical Vein Endothelial Cells (HUVEC) healthy cell line. The MRN loading and release characteristics of HPAC@MNPs were analyzed. The results indicated that the enclosed medication exhibited a prolonged spreading time during release. In summary, the use of HPAC@MNPs magnetic nanocomposite carriers may have great potential to effectively deliver MRN drugs to specific sites.

Anahtar Kelimeler

• FTIR
• HUVEC
• MCF-7
• U373
• Activated carbon
• Nanoparticles

Morin hidrat yüklü HPAC@MNP nanokompozitinin hazırlanması, karakterizasyonu ve in vitro uygulamaları

Öz

Kanser, her yıl küresel olarak milyonlarca insanın hayatına mal olan, tüm yaş gruplarından ve etnik kökenlerden bireyleri etkileyen yaygın ve ölümcül bir hastalıktır. Kanser tedavisinde kullanılan ilaçlar ayrım gözetmeksizin hem kötü huylu hem de sağlıklı hücreleri etkilediği için, büyük olumsuz etkilere neden olmasının yanı sıra sistemik toksisiteye de neden olur. Son zamanlarda, ilaçların neden olduğu olumsuz etkileri en aza indirmek için belirli bölgeleri özel olarak hedef alan ilaç verme sistemleri geliştirildi. Morin (MRN), antianjiyojenik, antiinflamatuar, antikanser ve antibakteriyel aktivitelerini araştırmak için yürütülen önemli araştırmaların konusu olan flavonol bazlı bir ilaçtır. Bu çalışma, alıç bitkisinden elde edilen biyouyumlu aktif karbon üzerine bir manyetik nanopartikül tabakası uygulanarak manyetik özelliklere sahip bir ürünün sentezini içermektedir. Alıç bitkisinden (HP) elde edilen aktif karbonun (AC) manyetik nanopartikülleri (MNP'ler) ile sentezlenen HPAC@MNP'lerin karakterizasyonu Fourier Transform Kızılötesi (FTIR), Taramalı Elektron Mikroskobu (SEM), Dinamik Işık Saçılımı (DLS) ve Zeta Potansiyeli ile karakterize edildi. DLS analizi, HPAC@MNP'lerin ve HPAC@MNPs-MRN'nin ortalama partikül boyutunun sırasıyla yaklaşık 105 nm ve 142 nm olduğunu hesaplandı. İlaç yüklü manyetik nanokompozit, MCF-7 (meme), U373 (Glioblastoma) kanser hücre hatları ve İnsan Göbek Kordonu Endotel Hücreleri (HUVEC) sağlıklı hücre hattı üzerindeki sitotoksik etkileri açısından değerlendirildi. HPAC@MNP'lerin MRN yükleme ve salım özellikleri analiz edildi. Sonuçlar, kapalı ilacın salım sırasında uzun bir yayılma süresi sergilediğini gösterdi. Özetle, HPAC@MNPs manyetik nanokompozit taşıyıcıların kullanımı, MRN ilaçlarını belirli bölgelere etkili bir şekilde ulaştırmak için büyük bir potansiyele sahip olabilir.

Anahtar Kelimeler

• FTIR
• HUVEC
• MCF-7
• U373
• Aktif karbon
• Nanopartikül

Kaynakça

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