Diosmin Flavanoid'in Bazı Metabolik Enzimler Üzerindeki İnhibisyon Etkisi ve Antioksidan Özelliklerinin İncelenmesi

Öz

Diosmin, flavanon glikozit hesperidin'in dehidrasyonu ile elde edilen doğal bir flavonoiddir. Bu çalışma, çeşitli sağlık sorunlarıyla bağlantılı olan insan karbonik anhidraz (hCA I ve hCA II) ve asetilkolinesteraz (AChE) enzimleri üzerinde diosmin flavonoidinin inhibisyon etkilerini değerlendirmiş ve bunları standart ilaçlarla karşılaştırmıştır. Antioksidan aktiviteyi belirlemek için, in vitro ABTS·+, DPPH radikal temizleme, bakır iyonu (Cu2+), FRAP ve Fe3+ indirgeme aktiviteleri ayrı ayrı gerçekleştirilmiştir. Butil hidroksianisol (BHA), trolox, α-tokoferol, butil hidroksitoluen (BHT) ve askorbik asit gibi referans antioksidanlar kullanılmıştır. Sonuçlar, diosminin güçlü bir antioksidan aktivite göstermediğini ortaya koymuştur. Ayrıca, diosminin hCA I, hCA II ve AChE enzimleri üzerindeki inhibisyon etkileri incelenmiştir. Diosmin, sırasıyla 3,84±0,63 ve 15,64±1,90 nM Ki değerleri ile hCA I ve hCA II enzimlerini etkili bir şekilde inhibe etti (Tablo 1). Standart ilaç AZA'nın Ki değerleri (Ki: 1,44±0,32 nM için hCA I- Ki: 3,66±0,25 nM için hCA II). Öte yandan, diosmin AChE enzimlerini 9,31±2,40 Ki değerleriyle etkili bir şekilde inhibe etti. Standart ilaç Tacrine'in AChE için Ki değerleri 6,22±1,95 nM'dir.

Anahtar Kelimeler

• Diosmin
• antioksidan
• Asetilkolinesteraz
• Karbonik anhidraz
• Enzim İnhibisyonu

Inhibition Effect of Diosmin Flavanoid on Some Metabolic Enzymes and Investigation of Antioxidant Properties

Öz

Diosmin is a natural flavonoid obtained by dehydrating flavanone glycoside hesperidin. This study evaluated the inhibition effects of diosmin flavonoid on human carbonic anhydrases (hCA I and hCA II) and acetylcholinesterase (AChE) enzymes, which are linked to various health issues, and compared the results with standard drugs. The antioxidant potential was assessed using five assays, namely DPPH, ABTS, FRAP, CUPRAC and Fe3+ reducing assays. The results determined that diosmin didn’t exhibit strong antioxidant activity. Additionally, the inhibition effects of diosmin on hCA I, hCA II, and AChE enzymes were examined. It effectively inhibited hCA I and hCA II enzymes with Ki values of 3.84±0.63 nM and 15.64±1.90 nM, respectively (Table 1). For comparison, the Ki values of the standard drug AZA were 1.44 ± 0.32 nM for hCA I and 3.66 ± 0.25 nM for hCA II. On the other hand, diosmin effectively inhibited AChE enzymes with Ki values of 9.31±2.40 nM. The Ki values of the standard drug Tacrine was 6.22±1.95 nM for AChE. These findings suggest that diosmin may serve as a selective enzyme inhibitor, particularly for hCA I, but has limited antioxidant capacity. Diosmin exhibited relatively weak antioxidant activity compared to standard antioxidants.

Anahtar Kelimeler

• Antioxidant
• Diosmin
• Acetylcholinesterase
• Carbonic anhydrase
• Enzyme Inhibition

Kaynakça

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