Limonium globuliferum ‘Un İn vitro Toksikolojik Etkileri

Öz

Bitkilerin etkinliğini araştırmak, geleneksel tıpta kullanımlarının güvenilirliğini değerlendirmek için önemlidir. Günümüzde bile, Limonium bitkisinin çeşitli türleri farklı hastalıkların tedavisinde alternatif bir araç olarak kullanılmaktadır. Bu çalışmanın amacı, alternatif tıpta Limonium cinsinin farklı türlerinin yanlış kullanımını önlemek için Limonium globuliferum'un aseton ekstraktlarının sitotoksik ve mutajenik etkilerini araştırmaktır. Bu çalışmada, L. globuliferum aseton ekstraktlarının mutajenikliği ve sitotoksisitesi, bakteriyel AMES testi ve memeli hücreleri ile yapılan MTT testi dahil olmak üzere in vitro test sistemleri kullanılarak belirlenmiştir. MTT testine göre, L. globuliferum'un kök ve gövdesinin aseton ekstraktları hücre çoğalması üzerinde olumsuz bir etkiye sahipken, yaprak ekstraktı bazı uygulamalarda hücreler üzerinde olumlu etkiler göstermiştir. AMES testi sonuçları da kök ve yaprağın aseton ekstraktlarının zayıf mutajenik etkileri olduğunu göstermiştir.

Anahtar Kelimeler

• Limonium globuliferum
• AMES test
• MTT testi
• sitotoksisite
• genotoksisite

In vitro Toxicologic Effects of Limonium globuliferum Acetone Extracts

Öz

Researching the effectiveness of plants is important for evaluating the reliability of their use in traditional medicine. Even today, various species of the Limonium plant are still used as an alternative tool in the treatment of different diseases. The aim of this study is to investigate the cytotoxic and mutagenic effects of acetone extracts of Limonium globuliferum to prevent the incorrect use of acetone extract of this species in alternative medicine. In this study, the mutagenicity and cytotoxicity of L. globuliferum acetone extracts were determined using in vitro test systems, including the bacterial AMES test and the MTT test performed with mammalian cells. According to the MTT test, the acetone extracts of the root have cytotoxic effects in all concentrations and exposure periods, stem extracts of L. globuliferum have a negative impact on cell proliferation except 72 h 3.125 µg/ml. Leaf extract showed positive effects on cells in all concentrations at 48 h applications. The AMES test results also indicated that the acetone extracts of the root and leaf have weak mutagenic effects as a result of the dose-dependent toxicity. On the other hand some of the concentrations has toxic effects on Salmonella strains and only 0,1 g/L leaf extract accepted as mutagenic.

Anahtar Kelimeler

• Limonium globuliferum
• AMES test
• MTT assay
• Cytotoxicity
• Genotoxicity
• Acetone extract

Kaynakça

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