Biochemical and Molecular Characterization of Lambda Cyhalothrin in Capoeta capoeta

Yıl 2018, , 57 - 63, 30.06.2018

Evren Koç , Mustafa Akçay

https://doi.org/10.21597/jist.427884

Öz

In this study, it was aimed the effects of Lambda Cyhalothrin (LCT) on Capoeta capoeta (Guldenstaedt

1773) caught from Kars river investigating by biochemical and molecular methods. The fishes, caught from Kars

river, were divided into 3 groups as 10 fishes in each group. The groups; control group (no application was applied),

the fishes in I. group 0.012 µgL-1 LCT, II. groups of fishes 0.025 µgL-1 LCT included to the tanks; duration of the

experiment was 6 hours. At the end of this period, blood and liver tissue samples were taken from the fishes for

biochemical and molecular analyzes. According to spectrophotometric anlyses, no statistically difference were

found between the control and application groups for the levels of Glutathione (GSH) (P> 0.05). 8-Hydroxy-2’-

Deoxyguanosine (8-OHdG) levels were decreased in the LCT-treated groups compared to the control group (P

<0.01). It was found that the expression levels of liver Glutathione S-transferase (GST), Glutathione peroxidase

(GPx), Glutathione Reductase (GR), Catalase (CAT) and Superoxide Dismutase (SOD) enzymes were increased

compared to control group according to RT-PCR method. As a conclusion, LCT showed toxic effects on Capoeta

capoeta fish species. Accordingly, increased antioxidant enzyme levels in fish, reducing oxidative damage and

DNA/RNA damage.

Anahtar Kelimeler

Antioxidant enzymes, fish, lambda cyhalothrin

Capoeta capoeta’da Lambda Cyhalothrin’in Biyokimyasal ve Moleküler Karakterizasyonu

Öz

Bu çalışmada, Kars Çayından yakalanan Capoeta capoeta (Guldenstaedt 1773) üzerine Lambda Cyhalothrin

(LCT)’in etkilerinin biyokimyasal ve moleküler yöntemlerle araştırılması amaçlandı. Kars Çayından yakalanan

balıklar her grupta 10 adet balık olacak şekilde 3 gruba ayrıldı. Gruplar; kontrol grubu (herhangi bir uygulama

yapılmadı), I. gruptaki balıklar 0.012 µgL-1 LCT içeren tankta, II. gruptaki balıklar ise 0.025 µgL-1 LCT içeren

tankta 6 saat süreyle bekletildi. Bu süre sonunda balıklardan biyokimyasal ve moleküler analizler için kan ve karaciğer

doku örnekleri alındı. Uygulanan spektrofotometrik analizlarin sonucu olarak Glutatyon (GSH) düzeyleri

için kontrol ve uygulama gruplarında istatistiksel farklılık saptanmadı (P>0.05). 8-Hidroksi-2’-Deoksiguanozin

(8-OHdG) düzeylerinin ise LCT uygulanan gruplarda kontrol grubuna göre azaldığı tespit edildi (P<0.01). Karaciğer

Glutatyon S-transferaz (GST), Glutatyon peroksidaz (GPx), Glutatyon Redüktaz (GR), Katalaz (CAT) ve

Süperoksit Dismutaz (SOD) enzimlerinin ekspresyon seviyeleri RT-PCR yöntemi ile araştırıldığında ise kontrol

grubuna göre artış meydana geldiği belirlendi. Sonuç olarak; LCT’in Capoeta capoeta balık türleri üzerinde toksik

etki gösterdiği, buna bağlı olarak balıklarda antioksidan enzim düzeyleri artarak oksidatif hasarı ve DNA/RNA

hasarını azalttığı tespit edildi.

Anahtar Kelimeler

Antioksidan enzimler, balık, lambda cyhalothrin

Kaynakça

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