Biochemical and Molecular Characterization of Lambda Cyhalothrin in Capoeta capoeta

Öz

In this study, it was aimed the effects of Lambda Cyhalothrin (LCT) on Capoeta capoeta (Guldenstaedt

1773) caught from Kars river investigating by biochemical and molecular methods. The fishes, caught from Kars

river, were divided into 3 groups as 10 fishes in each group. The groups; control group (no application was applied),

the fishes in I. group 0.012 µgL-1 LCT, II. groups of fishes 0.025 µgL-1 LCT included to the tanks; duration of the

experiment was 6 hours. At the end of this period, blood and liver tissue samples were taken from the fishes for

biochemical and molecular analyzes. According to spectrophotometric anlyses, no statistically difference were

found between the control and application groups for the levels of Glutathione (GSH) (P> 0.05). 8-Hydroxy-2’-

Deoxyguanosine (8-OHdG) levels were decreased in the LCT-treated groups compared to the control group (P

<0.01). It was found that the expression levels of liver Glutathione S-transferase (GST), Glutathione peroxidase

(GPx), Glutathione Reductase (GR), Catalase (CAT) and Superoxide Dismutase (SOD) enzymes were increased

compared to control group according to RT-PCR method. As a conclusion, LCT showed toxic effects on Capoeta

capoeta fish species. Accordingly, increased antioxidant enzyme levels in fish, reducing oxidative damage and

DNA/RNA damage.

Anahtar Kelimeler

• Antioxidant enzymes,fish,lambda cyhalothrin

Capoeta capoeta’da Lambda Cyhalothrin’in Biyokimyasal ve Moleküler Karakterizasyonu

Öz

Bu çalışmada, Kars Çayından yakalanan Capoeta capoeta (Guldenstaedt 1773) üzerine Lambda Cyhalothrin

(LCT)’in etkilerinin biyokimyasal ve moleküler yöntemlerle araştırılması amaçlandı. Kars Çayından yakalanan

balıklar her grupta 10 adet balık olacak şekilde 3 gruba ayrıldı. Gruplar; kontrol grubu (herhangi bir uygulama

yapılmadı), I. gruptaki balıklar 0.012 µgL-1 LCT içeren tankta, II. gruptaki balıklar ise 0.025 µgL-1 LCT içeren

tankta 6 saat süreyle bekletildi. Bu süre sonunda balıklardan biyokimyasal ve moleküler analizler için kan ve karaciğer

doku örnekleri alındı. Uygulanan spektrofotometrik analizlarin sonucu olarak Glutatyon (GSH) düzeyleri

için kontrol ve uygulama gruplarında istatistiksel farklılık saptanmadı (P>0.05). 8-Hidroksi-2’-Deoksiguanozin

(8-OHdG) düzeylerinin ise LCT uygulanan gruplarda kontrol grubuna göre azaldığı tespit edildi (P<0.01). Karaciğer

Glutatyon S-transferaz (GST), Glutatyon peroksidaz (GPx), Glutatyon Redüktaz (GR), Katalaz (CAT) ve

Süperoksit Dismutaz (SOD) enzimlerinin ekspresyon seviyeleri RT-PCR yöntemi ile araştırıldığında ise kontrol

grubuna göre artış meydana geldiği belirlendi. Sonuç olarak; LCT’in Capoeta capoeta balık türleri üzerinde toksik

etki gösterdiği, buna bağlı olarak balıklarda antioksidan enzim düzeyleri artarak oksidatif hasarı ve DNA/RNA

hasarını azalttığı tespit edildi.

Anahtar Kelimeler

• Antioksidan enzimler,balık,lambda cyhalothrin

Kaynakça

1. Abu-Qare A, Abou-Donia M. 2000. Increased 8-hydroxy-2′-deoxyguanosine, a biomarker of oxidative DNA damage in rat urine following a single dermal dose of DEET (N,N-diethyl-m-toluamide), and permethrin, alone and in combination. Toxicology Letters 117: 151–160.
2. Ağar S, Aydınoğlu H, Temel O, İkizünal K, Ece H. 1991. Pestisit kullanımının tarihçesi, bugünü ve geleceği. Türk. entomol. derg., 15: 247–256.
3. Ahmed MM, Nasr SA. 2015. Protective effect of broccoli and ferulic acid on imidacloprid induced neurotoxicity in rats. Journal of Biomedical and Pharmaceutical Research 4: 82–89.
4. Ansari S, Ansari BA. 2014a. Temporal variations of CAT, GSH, and LPO in gills and livers of zebrafish, Danio rerio, exposed to dimethoate. Arch. Pol. Fish. 22: 101–109.
5. Ansari S, Ansari BA. 2014b. Toxic effect of Alphamethrin on catalase, reduced glutathione and lipid peroxidation in the gill and liver of zebrafish, danio rerio. World Journal of Zoology 9: 155–161.
6. Antica M, Paradzik M, Novak S, Dzebro S, Dominis M. 2010. Gene expression in formalin-fixed paraffin-embedded lymph nodes. Journal of immunological methods 359: 42–46.
7. Calviello G, Piccioni E, Boninsegna A, Tedesco B, Maggiano N, Serini S, Wolf FI, Palozza P. 2006. DNA damage and apoptosis induction by the pesticide Mancozeb in rat cells: Involvement of the oxidative mechanism. Toxicology and Applied Pharmacology 211: 87–96.
8. Dahlhaus M, Almstadt E, Henschke P, Lüttgert S, Appel KE. 1995. Induction of 8-hydroxy-2-deoxyguanosine and single-strand breaks in DNA of V79 cells by tetrachloro-p-hydroquinone. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 329: 29–36.

9. De Martinis BS, Marıa De Lourdes PB. 2002. Methodology for urınary 8-hydroxy-2′-deoxyguanosıne analysıs by hplc wıth electrochemıcal detectıon. 46: 129–131.
10. Ding G, Han S, Wang P, Gao Y, Shi R, Wang G, Tian Y. 2012. Increased levels of 8-hydroxy-2′-deoxyguanosine are attributable to organophosphate pesticide exposure among young children. Environmental Pollution 167: 110–114.
11. Fetoui H, Makni M, Garoui EM, Zeghal N. 2010. Toxic effects of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the rat kidney: Involvement of oxidative stress and protective role of ascorbic acid. Experimental and Toxicologic Pathology 62: 593–599.
12. Gowri B, Govindassamy P, Ramalingam V. 2013. Influence of Cypermethrin on DNA and RNA content in different organs of fresh water fish Cyprinus carpio. Iranian Journal of Pharmaceutical Sciences 9: 1–10.
13. Halliwell B. 1991. Drug Antioxidant Effects. Drugs 42: 569–605.
14. Helbock HJ, Beckman KB, Ames BN. 1999. 8-Hydroxydeoxyguanosine and 8-hydroxyguanine as biomarkers of oxidative DNA damage (BM in Enzymology, Ed.). Methods in Enzymology 300: 156–166.
15. Johansen SJ, Harris AK, Rychly DJ, Ergul A. 2005. Oxidative stress and the use of an tioxidants in diabetes: Linking basic science to clinical practice. Cardiovascular Diabetology 4.
16. Kumar A, Sharma B, Pandey RS. 2010. Toxicological assessment of pyrethroid insecticides with special reference to cypermethrin and cyhalothrin in freshwater fishes, International Journal of Biological Medical Research. BioMed SciDirect Publications 1: 315–325.
17. Kutluyer F, Erişir M, Benzer F, Öğretmen F, İnanan BE. 2015. The in vitro effect of Lambda-cyhalothrin on quality and antioxidant responses of rainbow trout Oncorhynchus mykiss spermatozoa. Environmental Toxicology and Pharmacology 40: 855–860.
18. Liu L, Zhu B, Gong YX, Liu GL, Wang GX. 2015. Neurotoxic effect of triazophos on goldfish (Carassius auratus) and tissue specific antioxidant responses. Ecotoxicology and Environmental Safety 116: 68–75.
19. Lodovici M, Aiolli S, Monserrat C, Dolara P, Medica A, Di Simplicio P. 1994. Effect of a mixture of 15 commonly used pesticides on DNA levels of 8-hydroxy-2-deoxyguanosine and xenobiotic metabolizing enzymes in rat liver. Journal of Environmental Pathology, Toxicology and Oncology: Official Organ of the International Society for Environmental Toxicology and Cancer 13: 163–168.
20. Mossa ATH, Swelam ES, Mohafrash SMM. 2015. Sub-chronic exposure to fipronil induced oxidative stress, biochemical and histopathological changes in the liver and kidney of male albino rats. Toxicology Reports 2: 775–784.
21. Ongley ED. 1996. Control of water pollution from agriculture. Roma: Daya Publishing House.
22. Portugal M, Barak V, Ginsburg I, Kohen R. 2007. Interplay among oxidants, antioxidants, and cytokines in skin disorders: Present status and future considerations. Biomedicine & Pharmacotherapy 61: 412–422.
23. Ribeiro Oliveira CA, Vollaire Y, Sanchez-Chardi A, Roche H. 2005. Bioaccumulation and the effects of organochlorine pesticides, PAH and heavy metals in the Eel (Anguilla anguilla) at the Camargue Nature Reserve, France. Aquatic Toxicology 74: 53–69.
24. Roth JR, Hughes KT. 1985. Directed formation of deletions and duplication using Mud(Ap, lac). Genetic 109: 263–282.
25. Sharma D, Sangha GK. 2014. Triazophos induced oxidative stress and histomorphological changes in liver and kidney of female albino rats. Pesticide Biochemistry and Physiology 110: 71–80.
26. Topal A, Alak G, Altun S, Erol HS, Atamanalp M. 2017a. Evaluation of 8-hydroxy-2-deoxyguanosine and NFkB activation, oxidative stress response, acetylcholinesterase activity, and histopathological changes in rainbow trout brain exposed to linuron. Environmental Toxicology and Pharmacology 49: 14–20.
27. Topal A, Alak G, Ozkaraca M, Yeltekin AC, Comaklı S, Acıl G, Kokturk M, Atamanalp M. 2017b. Neurotoxic responses in brain tissues of rainbow trout exposed to imidacloprid pesticide: Assessment of 8-hydroxy-2-deoxyguanosine activity, oxidative stress and acetylcholinesterase activity. Chemosphere 175: 186–191.
28. Toth SJ, Sparks TC. 1990. Effect of Temperature on Toxicity and Knockdown Activity of cis-Permethrin, Esfenvalerate, and λ-Cyhalothrin in the Cabbage Looper (Lepidoptera: Noctuidae). Journal of Economic Entomology 83: 342–346.
29. Wang Q, Xia X, Deng X, Li N, Wu D, Zhang L, Yang C, Tao F, Zhou J. 2016. Lambda-cyhalothrin disrupts the up-regulation effect of 17β-estradiol on post-synaptic density 95 protein expression via estrogen receptor α-dependent Akt pathway. Journal of Environmental Sciences 41: 252–260.